Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS26525 and RR42_RS26530 are separated by 106 nucleotides RR42_RS26530 and RR42_RS26535 are separated by 110 nucleotides RR42_RS26535 and RR42_RS26540 overlap by 4 nucleotides
RR42_RS26525: RR42_RS26525 - IclR family transcriptional regulator, at 1,307,499 to 1,308,275
_RS26525
RR42_RS26530: RR42_RS26530 - LacI family transcriptional regulator, at 1,308,382 to 1,309,356
_RS26530
RR42_RS26535: RR42_RS26535 - diguanylate cyclase, at 1,309,467 to 1,310,150
_RS26535
RR42_RS26540: RR42_RS26540 - 3-phosphoglycerate dehydrogenase, at 1,310,147 to 1,311,082
_RS26540
Position (kb)
1308
1309
1310 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1307.569 kb on + strand at 1307.569 kb on + strand at 1307.569 kb on + strand at 1307.569 kb on + strand at 1307.570 kb on - strand at 1307.570 kb on - strand at 1307.703 kb on + strand, within RR42_RS26525 at 1307.747 kb on - strand, within RR42_RS26525 at 1307.770 kb on + strand, within RR42_RS26525 at 1307.923 kb on + strand, within RR42_RS26525 at 1307.924 kb on - strand, within RR42_RS26525 at 1308.007 kb on + strand, within RR42_RS26525 at 1308.008 kb on - strand, within RR42_RS26525 at 1308.073 kb on + strand, within RR42_RS26525 at 1308.119 kb on + strand, within RR42_RS26525 at 1308.119 kb on + strand, within RR42_RS26525 at 1308.119 kb on + strand, within RR42_RS26525 at 1308.119 kb on + strand, within RR42_RS26525 at 1308.120 kb on - strand, within RR42_RS26525 at 1308.643 kb on + strand, within RR42_RS26530 at 1308.643 kb on + strand, within RR42_RS26530 at 1308.644 kb on - strand, within RR42_RS26530 at 1308.695 kb on - strand, within RR42_RS26530 at 1308.709 kb on + strand, within RR42_RS26530 at 1308.728 kb on + strand, within RR42_RS26530 at 1308.730 kb on - strand, within RR42_RS26530 at 1308.790 kb on + strand, within RR42_RS26530 at 1308.934 kb on + strand, within RR42_RS26530 at 1308.934 kb on + strand, within RR42_RS26530 at 1308.934 kb on + strand, within RR42_RS26530 at 1308.935 kb on - strand, within RR42_RS26530 at 1308.935 kb on - strand, within RR42_RS26530 at 1308.988 kb on + strand, within RR42_RS26530 at 1308.988 kb on + strand, within RR42_RS26530 at 1308.988 kb on + strand, within RR42_RS26530 at 1308.989 kb on - strand, within RR42_RS26530 at 1308.989 kb on - strand, within RR42_RS26530 at 1308.989 kb on - strand, within RR42_RS26530 at 1309.020 kb on + strand, within RR42_RS26530 at 1309.260 kb on - strand at 1309.260 kb on - strand at 1309.383 kb on + strand at 1309.391 kb on + strand at 1309.392 kb on - strand at 1309.392 kb on - strand at 1309.392 kb on - strand at 1309.399 kb on + strand at 1309.399 kb on + strand at 1309.399 kb on + strand at 1309.399 kb on + strand at 1309.400 kb on - strand at 1309.400 kb on - strand at 1309.400 kb on - strand at 1309.423 kb on + strand at 1309.424 kb on - strand at 1309.424 kb on - strand at 1309.613 kb on + strand, within RR42_RS26535 at 1309.614 kb on - strand, within RR42_RS26535 at 1309.614 kb on - strand, within RR42_RS26535 at 1309.848 kb on + strand, within RR42_RS26535 at 1310.309 kb on + strand, within RR42_RS26540
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,307,569 + -0.4 1,307,569 + -0.1 1,307,569 + +0.7 1,307,569 + +0.3 1,307,570 - +0.0 1,307,570 - +1.1 1,307,703 + RR42_RS26525 0.26 -1.8 1,307,747 - RR42_RS26525 0.32 -2.0 1,307,770 + RR42_RS26525 0.35 -0.7 1,307,923 + RR42_RS26525 0.55 -0.3 1,307,924 - RR42_RS26525 0.55 +0.8 1,308,007 + RR42_RS26525 0.65 +0.8 1,308,008 - RR42_RS26525 0.66 +1.0 1,308,073 + RR42_RS26525 0.74 +1.1 1,308,119 + RR42_RS26525 0.80 +1.1 1,308,119 + RR42_RS26525 0.80 -1.0 1,308,119 + RR42_RS26525 0.80 -0.2 1,308,119 + RR42_RS26525 0.80 -0.8 1,308,120 - RR42_RS26525 0.80 +0.7 1,308,643 + RR42_RS26530 0.27 +1.1 1,308,643 + RR42_RS26530 0.27 +0.3 1,308,644 - RR42_RS26530 0.27 -0.0 1,308,695 - RR42_RS26530 0.32 -0.2 1,308,709 + RR42_RS26530 0.34 -1.0 1,308,728 + RR42_RS26530 0.35 -0.4 1,308,730 - RR42_RS26530 0.36 +0.6 1,308,790 + RR42_RS26530 0.42 -0.5 1,308,934 + RR42_RS26530 0.57 +0.3 1,308,934 + RR42_RS26530 0.57 -0.8 1,308,934 + RR42_RS26530 0.57 +0.0 1,308,935 - RR42_RS26530 0.57 +1.7 1,308,935 - RR42_RS26530 0.57 -0.6 1,308,988 + RR42_RS26530 0.62 -0.2 1,308,988 + RR42_RS26530 0.62 +1.8 1,308,988 + RR42_RS26530 0.62 +0.3 1,308,989 - RR42_RS26530 0.62 +0.7 1,308,989 - RR42_RS26530 0.62 -0.7 1,308,989 - RR42_RS26530 0.62 -1.0 1,309,020 + RR42_RS26530 0.65 -1.9 1,309,260 - +0.4 1,309,260 - +0.2 1,309,383 + -0.5 1,309,391 + +0.1 1,309,392 - -0.3 1,309,392 - +0.5 1,309,392 - -1.0 1,309,399 + -0.3 1,309,399 + -0.5 1,309,399 + -0.7 1,309,399 + +1.7 1,309,400 - +1.0 1,309,400 - +0.2 1,309,400 - -0.7 1,309,423 + +0.4 1,309,424 - +0.8 1,309,424 - +0.2 1,309,613 + RR42_RS26535 0.21 +0.1 1,309,614 - RR42_RS26535 0.21 +0.1 1,309,614 - RR42_RS26535 0.21 +0.3 1,309,848 + RR42_RS26535 0.56 +0.4 1,310,309 + RR42_RS26540 0.17 +1.3
Or see this region's nucleotide sequence