Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS24745

Experiment: R2A with Vancomycin 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS24740 and RR42_RS24745 are separated by 99 nucleotidesRR42_RS24745 and RR42_RS24750 are separated by 251 nucleotidesRR42_RS24750 and RR42_RS24755 are separated by 140 nucleotides RR42_RS24740: RR42_RS24740 - hydrolase, at 887,872 to 888,567 _RS24740 RR42_RS24745: RR42_RS24745 - hypothetical protein, at 888,667 to 889,308 _RS24745 RR42_RS24750: RR42_RS24750 - LysR family transcriptional regulator, at 889,560 to 889,904 _RS24750 RR42_RS24755: RR42_RS24755 - ATP synthase subunit beta, at 890,045 to 893,467 _RS24755 Position (kb) 888 889 890Strain fitness (log2 ratio) -2 -1 0 1at 887.671 kb on - strandat 887.702 kb on + strandat 887.703 kb on - strandat 887.744 kb on - strandat 887.756 kb on - strandat 887.756 kb on - strandat 887.756 kb on - strandat 887.756 kb on - strandat 887.756 kb on - strandat 887.756 kb on - strandat 887.860 kb on + strandat 887.918 kb on + strandat 887.959 kb on - strand, within RR42_RS24740at 887.960 kb on + strand, within RR42_RS24740at 887.960 kb on + strand, within RR42_RS24740at 887.975 kb on - strand, within RR42_RS24740at 888.052 kb on + strand, within RR42_RS24740at 888.053 kb on - strand, within RR42_RS24740at 888.170 kb on - strand, within RR42_RS24740at 888.170 kb on - strand, within RR42_RS24740at 888.202 kb on + strand, within RR42_RS24740at 888.202 kb on + strand, within RR42_RS24740at 888.203 kb on - strand, within RR42_RS24740at 888.203 kb on - strand, within RR42_RS24740at 888.299 kb on + strand, within RR42_RS24740at 888.303 kb on - strand, within RR42_RS24740at 888.324 kb on + strand, within RR42_RS24740at 888.324 kb on + strand, within RR42_RS24740at 888.324 kb on + strand, within RR42_RS24740at 888.324 kb on + strand, within RR42_RS24740at 888.324 kb on + strand, within RR42_RS24740at 888.325 kb on - strand, within RR42_RS24740at 888.325 kb on - strand, within RR42_RS24740at 888.325 kb on - strand, within RR42_RS24740at 888.373 kb on - strand, within RR42_RS24740at 888.373 kb on - strand, within RR42_RS24740at 888.417 kb on + strand, within RR42_RS24740at 888.417 kb on + strand, within RR42_RS24740at 888.417 kb on + strand, within RR42_RS24740at 888.573 kb on + strandat 888.573 kb on + strandat 888.574 kb on - strandat 888.574 kb on - strandat 888.574 kb on - strandat 888.636 kb on - strandat 888.636 kb on - strandat 888.636 kb on - strandat 888.636 kb on - strandat 888.663 kb on - strandat 888.755 kb on + strand, within RR42_RS24745at 888.756 kb on - strand, within RR42_RS24745at 888.756 kb on - strand, within RR42_RS24745at 888.756 kb on - strand, within RR42_RS24745at 888.895 kb on + strand, within RR42_RS24745at 888.908 kb on + strand, within RR42_RS24745at 888.909 kb on - strand, within RR42_RS24745at 889.024 kb on - strand, within RR42_RS24745at 889.134 kb on - strand, within RR42_RS24745at 889.137 kb on - strand, within RR42_RS24745at 889.137 kb on - strand, within RR42_RS24745at 889.137 kb on - strand, within RR42_RS24745at 889.169 kb on + strand, within RR42_RS24745at 889.170 kb on - strand, within RR42_RS24745at 889.170 kb on - strand, within RR42_RS24745at 889.170 kb on - strand, within RR42_RS24745at 889.170 kb on - strand, within RR42_RS24745at 889.366 kb on - strandat 889.373 kb on + strandat 889.373 kb on + strandat 889.446 kb on + strandat 889.446 kb on + strandat 889.447 kb on - strandat 889.459 kb on + strandat 889.470 kb on + strandat 889.521 kb on + strandat 889.521 kb on + strandat 889.521 kb on + strandat 889.521 kb on + strandat 889.572 kb on + strandat 889.572 kb on + strandat 889.572 kb on + strandat 889.572 kb on + strandat 889.573 kb on - strandat 889.650 kb on + strand, within RR42_RS24750at 889.715 kb on - strand, within RR42_RS24750at 889.715 kb on - strand, within RR42_RS24750at 889.792 kb on - strand, within RR42_RS24750at 889.847 kb on - strand, within RR42_RS24750at 889.847 kb on - strand, within RR42_RS24750at 889.936 kb on + strandat 890.007 kb on - strandat 890.007 kb on - strandat 890.056 kb on + strandat 890.057 kb on - strandat 890.057 kb on - strandat 890.226 kb on + strandat 890.243 kb on + strandat 890.244 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml
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887,671 - +1.6
887,702 + +0.2
887,703 - -0.2
887,744 - +1.1
887,756 - +1.7
887,756 - +0.2
887,756 - +1.4
887,756 - -0.3
887,756 - +0.0
887,756 - -0.8
887,860 + -0.1
887,918 + +0.4
887,959 - RR42_RS24740 0.12 -0.2
887,960 + RR42_RS24740 0.13 +0.4
887,960 + RR42_RS24740 0.13 -0.6
887,975 - RR42_RS24740 0.15 +1.1
888,052 + RR42_RS24740 0.26 -0.5
888,053 - RR42_RS24740 0.26 -0.7
888,170 - RR42_RS24740 0.43 -0.7
888,170 - RR42_RS24740 0.43 -0.1
888,202 + RR42_RS24740 0.47 -0.5
888,202 + RR42_RS24740 0.47 +0.4
888,203 - RR42_RS24740 0.48 -0.5
888,203 - RR42_RS24740 0.48 -0.0
888,299 + RR42_RS24740 0.61 +0.6
888,303 - RR42_RS24740 0.62 -1.4
888,324 + RR42_RS24740 0.65 -0.2
888,324 + RR42_RS24740 0.65 +1.3
888,324 + RR42_RS24740 0.65 -0.6
888,324 + RR42_RS24740 0.65 -0.3
888,324 + RR42_RS24740 0.65 -0.6
888,325 - RR42_RS24740 0.65 -0.0
888,325 - RR42_RS24740 0.65 -0.7
888,325 - RR42_RS24740 0.65 -1.2
888,373 - RR42_RS24740 0.72 +0.1
888,373 - RR42_RS24740 0.72 +1.0
888,417 + RR42_RS24740 0.78 +0.1
888,417 + RR42_RS24740 0.78 +0.9
888,417 + RR42_RS24740 0.78 +0.0
888,573 + -0.1
888,573 + +1.5
888,574 - -0.0
888,574 - +0.1
888,574 - -1.3
888,636 - -0.3
888,636 - -0.4
888,636 - -1.2
888,636 - -0.8
888,663 - +0.4
888,755 + RR42_RS24745 0.14 +0.0
888,756 - RR42_RS24745 0.14 +1.7
888,756 - RR42_RS24745 0.14 -0.5
888,756 - RR42_RS24745 0.14 -0.1
888,895 + RR42_RS24745 0.36 -0.5
888,908 + RR42_RS24745 0.38 -1.0
888,909 - RR42_RS24745 0.38 +0.7
889,024 - RR42_RS24745 0.56 -0.2
889,134 - RR42_RS24745 0.73 -0.3
889,137 - RR42_RS24745 0.73 -0.1
889,137 - RR42_RS24745 0.73 -0.1
889,137 - RR42_RS24745 0.73 +0.3
889,169 + RR42_RS24745 0.78 -0.6
889,170 - RR42_RS24745 0.78 -0.1
889,170 - RR42_RS24745 0.78 -2.1
889,170 - RR42_RS24745 0.78 +0.4
889,170 - RR42_RS24745 0.78 +0.3
889,366 - +0.4
889,373 + -0.7
889,373 + -0.1
889,446 + +0.9
889,446 + +1.2
889,447 - +0.0
889,459 + -0.2
889,470 + -1.4
889,521 + -0.1
889,521 + -2.0
889,521 + +0.4
889,521 + -0.3
889,572 + -1.8
889,572 + +0.8
889,572 + +0.2
889,572 + -0.3
889,573 - -0.3
889,650 + RR42_RS24750 0.26 -0.8
889,715 - RR42_RS24750 0.45 -0.2
889,715 - RR42_RS24750 0.45 +0.2
889,792 - RR42_RS24750 0.67 +0.9
889,847 - RR42_RS24750 0.83 -0.4
889,847 - RR42_RS24750 0.83 -0.2
889,936 + +0.0
890,007 - +0.5
890,007 - +0.2
890,056 + -1.0
890,057 - -0.4
890,057 - -1.0
890,226 + -0.7
890,243 + -0.4
890,244 - +0.5

Or see this region's nucleotide sequence