Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS24300 and RR42_RS24305 are separated by 18 nucleotides
RR42_RS24300: RR42_RS24300 - acetyl-CoA acetyltransferase, at 779,910 to 781,097
_RS24300
RR42_RS24305: RR42_RS24305 - 3-hydroxybutyryl-CoA dehydrogenase, at 781,116 to 781,967
_RS24305
Position (kb)
779
780
781
782 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 779.139 kb on + strand at 779.140 kb on - strand at 779.140 kb on - strand at 779.254 kb on - strand at 779.331 kb on - strand at 779.427 kb on + strand at 779.501 kb on + strand at 779.501 kb on + strand at 779.501 kb on + strand at 779.501 kb on + strand at 779.502 kb on - strand at 779.536 kb on + strand at 779.536 kb on + strand at 779.536 kb on + strand at 779.536 kb on + strand at 779.536 kb on + strand at 779.536 kb on + strand at 779.536 kb on + strand at 779.536 kb on + strand at 779.585 kb on - strand at 779.585 kb on - strand at 779.585 kb on - strand at 779.596 kb on + strand at 779.667 kb on + strand at 779.668 kb on - strand at 779.668 kb on - strand at 779.668 kb on - strand at 779.668 kb on - strand at 779.700 kb on + strand at 779.700 kb on + strand at 779.701 kb on - strand at 779.701 kb on - strand at 779.704 kb on + strand at 779.704 kb on + strand at 779.704 kb on + strand at 779.762 kb on - strand at 779.813 kb on + strand at 779.814 kb on - strand at 779.814 kb on - strand at 779.814 kb on - strand at 779.848 kb on + strand at 779.850 kb on - strand at 779.850 kb on - strand at 779.861 kb on + strand at 779.862 kb on - strand at 779.893 kb on + strand at 779.928 kb on + strand at 779.928 kb on + strand at 779.928 kb on + strand at 779.928 kb on + strand at 779.929 kb on - strand at 779.929 kb on - strand at 779.929 kb on - strand at 779.961 kb on - strand at 779.978 kb on + strand at 779.986 kb on + strand at 779.986 kb on + strand at 779.987 kb on - strand at 779.987 kb on - strand at 779.987 kb on - strand at 780.108 kb on + strand, within RR42_RS24300 at 780.108 kb on + strand, within RR42_RS24300 at 780.108 kb on + strand, within RR42_RS24300 at 780.108 kb on + strand, within RR42_RS24300 at 780.108 kb on + strand, within RR42_RS24300 at 780.108 kb on + strand, within RR42_RS24300 at 780.109 kb on - strand, within RR42_RS24300 at 780.109 kb on - strand, within RR42_RS24300 at 780.109 kb on - strand, within RR42_RS24300 at 780.109 kb on - strand, within RR42_RS24300 at 780.109 kb on - strand, within RR42_RS24300 at 780.109 kb on - strand, within RR42_RS24300 at 780.109 kb on - strand, within RR42_RS24300 at 780.285 kb on + strand, within RR42_RS24300 at 780.286 kb on + strand, within RR42_RS24300 at 780.286 kb on - strand, within RR42_RS24300 at 780.286 kb on - strand, within RR42_RS24300 at 780.286 kb on - strand, within RR42_RS24300 at 780.293 kb on - strand, within RR42_RS24300 at 780.342 kb on + strand, within RR42_RS24300 at 780.343 kb on - strand, within RR42_RS24300 at 780.344 kb on + strand, within RR42_RS24300 at 780.344 kb on + strand, within RR42_RS24300 at 780.344 kb on + strand, within RR42_RS24300 at 780.344 kb on + strand, within RR42_RS24300 at 780.345 kb on - strand, within RR42_RS24300 at 780.345 kb on - strand, within RR42_RS24300 at 780.345 kb on - strand, within RR42_RS24300 at 780.345 kb on - strand, within RR42_RS24300 at 780.345 kb on - strand, within RR42_RS24300 at 780.345 kb on - strand, within RR42_RS24300 at 780.403 kb on - strand, within RR42_RS24300 at 780.403 kb on - strand, within RR42_RS24300 at 780.403 kb on - strand, within RR42_RS24300 at 780.501 kb on - strand, within RR42_RS24300 at 780.547 kb on + strand, within RR42_RS24300 at 780.547 kb on + strand, within RR42_RS24300 at 780.548 kb on - strand, within RR42_RS24300 at 780.548 kb on - strand, within RR42_RS24300 at 780.548 kb on - strand, within RR42_RS24300 at 780.548 kb on - strand, within RR42_RS24300 at 780.548 kb on - strand, within RR42_RS24300 at 780.640 kb on + strand, within RR42_RS24300 at 780.685 kb on + strand, within RR42_RS24300 at 780.686 kb on - strand, within RR42_RS24300 at 780.686 kb on - strand, within RR42_RS24300 at 780.686 kb on - strand, within RR42_RS24300 at 780.688 kb on + strand, within RR42_RS24300 at 780.688 kb on + strand, within RR42_RS24300 at 780.689 kb on - strand, within RR42_RS24300 at 780.838 kb on + strand, within RR42_RS24300 at 780.838 kb on + strand, within RR42_RS24300 at 780.838 kb on + strand, within RR42_RS24300 at 780.853 kb on + strand, within RR42_RS24300 at 780.853 kb on + strand, within RR42_RS24300 at 780.853 kb on + strand, within RR42_RS24300 at 780.854 kb on - strand, within RR42_RS24300 at 780.888 kb on + strand, within RR42_RS24300 at 780.888 kb on + strand, within RR42_RS24300 at 780.889 kb on - strand, within RR42_RS24300 at 780.889 kb on - strand, within RR42_RS24300 at 780.928 kb on + strand, within RR42_RS24300 at 780.929 kb on - strand, within RR42_RS24300 at 781.005 kb on + strand at 781.006 kb on - strand at 781.006 kb on - strand at 781.006 kb on - strand at 781.006 kb on - strand at 781.006 kb on - strand at 781.006 kb on - strand at 781.021 kb on + strand at 781.022 kb on - strand at 781.070 kb on + strand at 781.070 kb on + strand at 781.122 kb on - strand at 781.130 kb on + strand at 781.130 kb on + strand at 781.131 kb on - strand at 781.131 kb on - strand at 781.166 kb on + strand at 781.167 kb on - strand at 781.231 kb on + strand, within RR42_RS24305 at 781.259 kb on + strand, within RR42_RS24305 at 781.259 kb on + strand, within RR42_RS24305 at 781.278 kb on - strand, within RR42_RS24305 at 781.335 kb on - strand, within RR42_RS24305 at 781.335 kb on - strand, within RR42_RS24305 at 781.335 kb on - strand, within RR42_RS24305 at 781.344 kb on + strand, within RR42_RS24305 at 781.346 kb on + strand, within RR42_RS24305 at 781.346 kb on + strand, within RR42_RS24305 at 781.347 kb on - strand, within RR42_RS24305 at 781.347 kb on - strand, within RR42_RS24305 at 781.347 kb on - strand, within RR42_RS24305 at 781.439 kb on - strand, within RR42_RS24305 at 781.439 kb on - strand, within RR42_RS24305 at 781.456 kb on + strand, within RR42_RS24305 at 781.651 kb on + strand, within RR42_RS24305 at 781.688 kb on + strand, within RR42_RS24305 at 781.689 kb on - strand, within RR42_RS24305 at 781.689 kb on - strand, within RR42_RS24305 at 781.706 kb on + strand, within RR42_RS24305 at 781.707 kb on - strand, within RR42_RS24305 at 781.830 kb on - strand, within RR42_RS24305 at 781.858 kb on - strand, within RR42_RS24305 at 781.900 kb on + strand at 781.900 kb on + strand at 781.901 kb on - strand at 781.901 kb on - strand at 781.901 kb on - strand at 781.934 kb on + strand at 781.935 kb on - strand at 781.935 kb on - strand at 781.935 kb on - strand at 781.953 kb on + strand at 781.953 kb on + strand at 781.953 kb on + strand at 781.953 kb on + strand at 781.953 kb on + strand at 781.954 kb on - strand at 781.954 kb on - strand at 781.954 kb on - strand at 781.959 kb on + strand at 781.959 kb on + strand at 781.959 kb on + strand at 781.959 kb on + strand at 781.978 kb on + strand at 781.978 kb on + strand at 781.992 kb on + strand at 781.993 kb on - strand at 782.057 kb on + strand at 782.057 kb on + strand at 782.057 kb on + strand at 782.058 kb on - strand at 782.076 kb on + strand at 782.076 kb on + strand at 782.076 kb on + strand at 782.076 kb on + strand at 782.076 kb on + strand at 782.077 kb on - strand at 782.077 kb on - strand at 782.077 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 779,139 + -1.0 779,140 - -0.8 779,140 - -1.0 779,254 - +0.2 779,331 - -0.1 779,427 + -0.2 779,501 + +0.1 779,501 + -0.1 779,501 + +0.4 779,501 + +0.0 779,502 - -1.5 779,536 + +1.5 779,536 + -0.2 779,536 + +0.7 779,536 + -1.5 779,536 + +0.3 779,536 + -0.4 779,536 + -2.9 779,536 + +1.7 779,585 - -1.7 779,585 - -0.7 779,585 - +0.9 779,596 + -1.1 779,667 + +1.3 779,668 - -0.8 779,668 - -1.9 779,668 - -0.1 779,668 - -0.4 779,700 + +0.2 779,700 + -1.3 779,701 - -0.8 779,701 - +0.8 779,704 + -0.4 779,704 + +0.1 779,704 + -1.9 779,762 - +0.6 779,813 + +1.1 779,814 - +1.0 779,814 - -0.4 779,814 - -0.2 779,848 + +0.2 779,850 - +0.7 779,850 - -1.2 779,861 + -0.2 779,862 - +0.3 779,893 + +1.3 779,928 + -1.0 779,928 + -0.1 779,928 + -1.0 779,928 + -0.2 779,929 - +0.3 779,929 - +0.1 779,929 - +0.5 779,961 - -0.7 779,978 + -0.6 779,986 + -0.3 779,986 + +1.1 779,987 - +1.3 779,987 - +1.1 779,987 - +0.5 780,108 + RR42_RS24300 0.17 -0.3 780,108 + RR42_RS24300 0.17 -0.4 780,108 + RR42_RS24300 0.17 -0.1 780,108 + RR42_RS24300 0.17 -0.1 780,108 + RR42_RS24300 0.17 -0.1 780,108 + RR42_RS24300 0.17 -0.7 780,109 - RR42_RS24300 0.17 -1.4 780,109 - RR42_RS24300 0.17 +1.6 780,109 - RR42_RS24300 0.17 -2.0 780,109 - RR42_RS24300 0.17 +0.3 780,109 - RR42_RS24300 0.17 -0.2 780,109 - RR42_RS24300 0.17 +0.1 780,109 - RR42_RS24300 0.17 +2.4 780,285 + RR42_RS24300 0.32 -0.7 780,286 + RR42_RS24300 0.32 +0.1 780,286 - RR42_RS24300 0.32 +0.3 780,286 - RR42_RS24300 0.32 +2.3 780,286 - RR42_RS24300 0.32 -0.1 780,293 - RR42_RS24300 0.32 +0.4 780,342 + RR42_RS24300 0.36 +2.1 780,343 - RR42_RS24300 0.36 +0.4 780,344 + RR42_RS24300 0.37 +0.9 780,344 + RR42_RS24300 0.37 +0.1 780,344 + RR42_RS24300 0.37 -0.6 780,344 + RR42_RS24300 0.37 -0.3 780,345 - RR42_RS24300 0.37 +1.2 780,345 - RR42_RS24300 0.37 +1.0 780,345 - RR42_RS24300 0.37 +1.7 780,345 - RR42_RS24300 0.37 +0.3 780,345 - RR42_RS24300 0.37 -0.1 780,345 - RR42_RS24300 0.37 +1.7 780,403 - RR42_RS24300 0.41 +0.9 780,403 - RR42_RS24300 0.41 +0.8 780,403 - RR42_RS24300 0.41 -0.9 780,501 - RR42_RS24300 0.50 +1.7 780,547 + RR42_RS24300 0.54 -1.7 780,547 + RR42_RS24300 0.54 +0.4 780,548 - RR42_RS24300 0.54 +0.2 780,548 - RR42_RS24300 0.54 -0.1 780,548 - RR42_RS24300 0.54 +0.2 780,548 - RR42_RS24300 0.54 +0.8 780,548 - RR42_RS24300 0.54 -0.9 780,640 + RR42_RS24300 0.61 -0.8 780,685 + RR42_RS24300 0.65 -2.3 780,686 - RR42_RS24300 0.65 -0.7 780,686 - RR42_RS24300 0.65 +0.7 780,686 - RR42_RS24300 0.65 -0.2 780,688 + RR42_RS24300 0.65 -2.7 780,688 + RR42_RS24300 0.65 +2.0 780,689 - RR42_RS24300 0.66 +0.6 780,838 + RR42_RS24300 0.78 +0.0 780,838 + RR42_RS24300 0.78 -0.3 780,838 + RR42_RS24300 0.78 -1.0 780,853 + RR42_RS24300 0.79 +1.9 780,853 + RR42_RS24300 0.79 +1.1 780,853 + RR42_RS24300 0.79 +0.5 780,854 - RR42_RS24300 0.79 -0.4 780,888 + RR42_RS24300 0.82 -0.8 780,888 + RR42_RS24300 0.82 +1.1 780,889 - RR42_RS24300 0.82 +0.7 780,889 - RR42_RS24300 0.82 -0.3 780,928 + RR42_RS24300 0.86 -0.5 780,929 - RR42_RS24300 0.86 +0.3 781,005 + +0.2 781,006 - -0.3 781,006 - -0.3 781,006 - -1.0 781,006 - +2.3 781,006 - +1.0 781,006 - +1.0 781,021 + -0.5 781,022 - -0.7 781,070 + +0.0 781,070 + -1.2 781,122 - +0.3 781,130 + -1.9 781,130 + -0.4 781,131 - +2.3 781,131 - +0.9 781,166 + -0.4 781,167 - -1.6 781,231 + RR42_RS24305 0.13 +0.3 781,259 + RR42_RS24305 0.17 +0.4 781,259 + RR42_RS24305 0.17 +0.4 781,278 - RR42_RS24305 0.19 +0.6 781,335 - RR42_RS24305 0.26 -0.1 781,335 - RR42_RS24305 0.26 -2.1 781,335 - RR42_RS24305 0.26 -1.2 781,344 + RR42_RS24305 0.27 +0.2 781,346 + RR42_RS24305 0.27 -0.2 781,346 + RR42_RS24305 0.27 +0.1 781,347 - RR42_RS24305 0.27 +1.3 781,347 - RR42_RS24305 0.27 -0.3 781,347 - RR42_RS24305 0.27 -0.1 781,439 - RR42_RS24305 0.38 +1.9 781,439 - RR42_RS24305 0.38 +0.3 781,456 + RR42_RS24305 0.40 +0.3 781,651 + RR42_RS24305 0.63 -0.7 781,688 + RR42_RS24305 0.67 -0.5 781,689 - RR42_RS24305 0.67 -1.0 781,689 - RR42_RS24305 0.67 -1.0 781,706 + RR42_RS24305 0.69 +0.4 781,707 - RR42_RS24305 0.69 -0.9 781,830 - RR42_RS24305 0.84 +2.7 781,858 - RR42_RS24305 0.87 +0.6 781,900 + -0.5 781,900 + -0.8 781,901 - -0.2 781,901 - +0.6 781,901 - +0.7 781,934 + +1.7 781,935 - +0.9 781,935 - +0.1 781,935 - -1.1 781,953 + -0.8 781,953 + -0.2 781,953 + +0.7 781,953 + +0.7 781,953 + +0.5 781,954 - +1.3 781,954 - -0.4 781,954 - -1.5 781,959 + +1.7 781,959 + +1.7 781,959 + -1.1 781,959 + -1.0 781,978 + -0.1 781,978 + -0.7 781,992 + +0.3 781,993 - -1.5 782,057 + -0.1 782,057 + -1.3 782,057 + -0.2 782,058 - -0.4 782,076 + +0.7 782,076 + -0.5 782,076 + +1.1 782,076 + +0.9 782,076 + -0.8 782,077 - -1.2 782,077 - -0.1 782,077 - -0.3
Or see this region's nucleotide sequence