Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS23395

Experiment: R2A with Vancomycin 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS23390 and RR42_RS23395 are separated by 24 nucleotidesRR42_RS23395 and RR42_RS23400 are separated by 213 nucleotides RR42_RS23390: RR42_RS23390 - hydrolase, at 575,376 to 576,239 _RS23390 RR42_RS23395: RR42_RS23395 - LysR family transcriptional regulator, at 576,264 to 577,151 _RS23395 RR42_RS23400: RR42_RS23400 - ABC transporter substrate-binding protein, at 577,365 to 578,354 _RS23400 Position (kb) 576 577 578Strain fitness (log2 ratio) -2 -1 0 1 2 3at 575.273 kb on - strandat 575.274 kb on + strandat 575.274 kb on + strandat 575.371 kb on + strandat 575.372 kb on - strandat 575.372 kb on - strandat 575.375 kb on + strandat 575.375 kb on + strandat 575.376 kb on - strandat 575.434 kb on - strandat 575.470 kb on + strand, within RR42_RS23390at 575.471 kb on - strand, within RR42_RS23390at 575.471 kb on - strand, within RR42_RS23390at 575.471 kb on - strand, within RR42_RS23390at 575.471 kb on - strand, within RR42_RS23390at 575.471 kb on - strand, within RR42_RS23390at 575.472 kb on + strand, within RR42_RS23390at 575.472 kb on + strand, within RR42_RS23390at 575.473 kb on - strand, within RR42_RS23390at 575.535 kb on - strand, within RR42_RS23390at 575.572 kb on - strand, within RR42_RS23390at 575.631 kb on - strand, within RR42_RS23390at 575.658 kb on - strand, within RR42_RS23390at 575.702 kb on - strand, within RR42_RS23390at 575.752 kb on - strand, within RR42_RS23390at 575.828 kb on + strand, within RR42_RS23390at 575.829 kb on - strand, within RR42_RS23390at 575.829 kb on - strand, within RR42_RS23390at 576.010 kb on + strand, within RR42_RS23390at 576.011 kb on - strand, within RR42_RS23390at 576.041 kb on + strand, within RR42_RS23390at 576.063 kb on + strand, within RR42_RS23390at 576.063 kb on + strand, within RR42_RS23390at 576.064 kb on - strand, within RR42_RS23390at 576.209 kb on + strandat 576.210 kb on - strandat 576.224 kb on - strandat 576.224 kb on - strandat 576.237 kb on + strandat 576.346 kb on + strandat 576.346 kb on + strandat 576.346 kb on + strandat 576.347 kb on - strandat 576.376 kb on - strand, within RR42_RS23395at 576.442 kb on - strand, within RR42_RS23395at 576.488 kb on + strand, within RR42_RS23395at 576.702 kb on - strand, within RR42_RS23395at 576.702 kb on - strand, within RR42_RS23395at 576.789 kb on - strand, within RR42_RS23395at 576.789 kb on - strand, within RR42_RS23395at 576.789 kb on - strand, within RR42_RS23395at 576.816 kb on - strand, within RR42_RS23395at 576.845 kb on - strand, within RR42_RS23395at 576.849 kb on - strand, within RR42_RS23395at 576.879 kb on - strand, within RR42_RS23395at 577.073 kb on + strandat 577.074 kb on - strandat 577.178 kb on - strandat 577.184 kb on - strandat 577.227 kb on + strandat 577.228 kb on - strandat 577.245 kb on + strandat 577.247 kb on + strandat 577.248 kb on - strandat 577.248 kb on - strandat 577.326 kb on - strandat 577.341 kb on - strandat 577.341 kb on - strandat 577.351 kb on - strandat 577.373 kb on - strandat 577.494 kb on + strand, within RR42_RS23400at 577.568 kb on + strand, within RR42_RS23400at 577.569 kb on - strand, within RR42_RS23400at 577.569 kb on - strand, within RR42_RS23400at 577.645 kb on - strand, within RR42_RS23400at 577.645 kb on - strand, within RR42_RS23400at 577.646 kb on + strand, within RR42_RS23400at 577.646 kb on + strand, within RR42_RS23400at 577.646 kb on + strand, within RR42_RS23400at 577.647 kb on - strand, within RR42_RS23400at 577.647 kb on - strand, within RR42_RS23400at 577.647 kb on - strand, within RR42_RS23400at 577.677 kb on + strand, within RR42_RS23400at 577.707 kb on + strand, within RR42_RS23400at 577.708 kb on - strand, within RR42_RS23400at 577.725 kb on + strand, within RR42_RS23400at 577.725 kb on + strand, within RR42_RS23400at 577.726 kb on - strand, within RR42_RS23400at 577.776 kb on - strand, within RR42_RS23400at 577.839 kb on + strand, within RR42_RS23400at 577.839 kb on + strand, within RR42_RS23400at 577.840 kb on - strand, within RR42_RS23400at 577.932 kb on + strand, within RR42_RS23400at 577.933 kb on - strand, within RR42_RS23400at 577.979 kb on - strand, within RR42_RS23400at 577.979 kb on - strand, within RR42_RS23400at 577.979 kb on - strand, within RR42_RS23400at 578.008 kb on + strand, within RR42_RS23400at 578.009 kb on - strand, within RR42_RS23400at 578.010 kb on + strand, within RR42_RS23400at 578.010 kb on + strand, within RR42_RS23400at 578.011 kb on - strand, within RR42_RS23400at 578.011 kb on - strand, within RR42_RS23400

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml
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575,273 - +0.1
575,274 + -1.3
575,274 + +0.2
575,371 + -0.1
575,372 - -0.1
575,372 - +1.9
575,375 + +0.3
575,375 + -0.5
575,376 - -2.3
575,434 - +1.0
575,470 + RR42_RS23390 0.11 +0.7
575,471 - RR42_RS23390 0.11 +0.8
575,471 - RR42_RS23390 0.11 +0.4
575,471 - RR42_RS23390 0.11 +1.0
575,471 - RR42_RS23390 0.11 +0.4
575,471 - RR42_RS23390 0.11 +0.6
575,472 + RR42_RS23390 0.11 -0.5
575,472 + RR42_RS23390 0.11 +1.4
575,473 - RR42_RS23390 0.11 -0.8
575,535 - RR42_RS23390 0.18 +0.7
575,572 - RR42_RS23390 0.23 -0.8
575,631 - RR42_RS23390 0.30 -1.3
575,658 - RR42_RS23390 0.33 +0.0
575,702 - RR42_RS23390 0.38 +1.4
575,752 - RR42_RS23390 0.44 +1.4
575,828 + RR42_RS23390 0.52 -1.6
575,829 - RR42_RS23390 0.52 +2.0
575,829 - RR42_RS23390 0.52 -0.1
576,010 + RR42_RS23390 0.73 -0.3
576,011 - RR42_RS23390 0.73 -0.3
576,041 + RR42_RS23390 0.77 -1.1
576,063 + RR42_RS23390 0.80 -0.7
576,063 + RR42_RS23390 0.80 -2.7
576,064 - RR42_RS23390 0.80 -1.1
576,209 + -2.0
576,210 - -1.0
576,224 - -0.5
576,224 - -0.5
576,237 + +0.6
576,346 + +0.5
576,346 + +0.2
576,346 + -0.4
576,347 - +0.5
576,376 - RR42_RS23395 0.13 -0.6
576,442 - RR42_RS23395 0.20 -0.6
576,488 + RR42_RS23395 0.25 -0.3
576,702 - RR42_RS23395 0.49 +1.6
576,702 - RR42_RS23395 0.49 -1.1
576,789 - RR42_RS23395 0.59 -0.8
576,789 - RR42_RS23395 0.59 +3.2
576,789 - RR42_RS23395 0.59 -0.6
576,816 - RR42_RS23395 0.62 +1.5
576,845 - RR42_RS23395 0.65 +0.2
576,849 - RR42_RS23395 0.66 +0.0
576,879 - RR42_RS23395 0.69 -0.2
577,073 + +0.7
577,074 - -2.4
577,178 - +0.8
577,184 - -0.4
577,227 + +0.0
577,228 - -0.8
577,245 + +0.3
577,247 + -0.4
577,248 - +0.1
577,248 - -0.7
577,326 - +0.0
577,341 - +1.9
577,341 - -0.1
577,351 - +0.9
577,373 - -0.7
577,494 + RR42_RS23400 0.13 -0.2
577,568 + RR42_RS23400 0.21 +0.1
577,569 - RR42_RS23400 0.21 -0.5
577,569 - RR42_RS23400 0.21 +1.3
577,645 - RR42_RS23400 0.28 +0.3
577,645 - RR42_RS23400 0.28 -0.4
577,646 + RR42_RS23400 0.28 -0.6
577,646 + RR42_RS23400 0.28 +0.3
577,646 + RR42_RS23400 0.28 +0.4
577,647 - RR42_RS23400 0.28 -0.5
577,647 - RR42_RS23400 0.28 +0.7
577,647 - RR42_RS23400 0.28 -1.5
577,677 + RR42_RS23400 0.32 -0.9
577,707 + RR42_RS23400 0.35 +0.2
577,708 - RR42_RS23400 0.35 +0.4
577,725 + RR42_RS23400 0.36 +0.8
577,725 + RR42_RS23400 0.36 -1.0
577,726 - RR42_RS23400 0.36 +0.2
577,776 - RR42_RS23400 0.42 +2.6
577,839 + RR42_RS23400 0.48 +1.6
577,839 + RR42_RS23400 0.48 -0.0
577,840 - RR42_RS23400 0.48 -2.6
577,932 + RR42_RS23400 0.57 +1.6
577,933 - RR42_RS23400 0.57 -1.1
577,979 - RR42_RS23400 0.62 -0.3
577,979 - RR42_RS23400 0.62 +1.4
577,979 - RR42_RS23400 0.62 +0.5
578,008 + RR42_RS23400 0.65 -0.4
578,009 - RR42_RS23400 0.65 -0.4
578,010 + RR42_RS23400 0.65 -0.8
578,010 + RR42_RS23400 0.65 +0.2
578,011 - RR42_RS23400 0.65 -1.1
578,011 - RR42_RS23400 0.65 -0.3

Or see this region's nucleotide sequence