Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23350 and RR42_RS23355 are separated by 126 nucleotides RR42_RS23355 and RR42_RS23360 are separated by 34 nucleotides RR42_RS23360 and RR42_RS23365 overlap by 4 nucleotides
RR42_RS23350: RR42_RS23350 - flagellar biosynthesis protein FliR, at 569,517 to 570,302
_RS23350
RR42_RS23355: RR42_RS23355 - flagellar biosynthetic protein FliQ, at 570,429 to 570,698
_RS23355
RR42_RS23360: RR42_RS23360 - flagellar biosynthesis protein flip, at 570,733 to 571,575
_RS23360
RR42_RS23365: RR42_RS23365 - flagellar biosynthesis protein FliO, at 571,572 to 572,042
_RS23365
Position (kb) 570
571 Strain fitness (log2 ratio) -2
-1
0
1 at 569.435 kb on + strand at 569.435 kb on + strand at 569.466 kb on + strand at 569.467 kb on - strand at 569.467 kb on - strand at 569.467 kb on - strand at 569.467 kb on - strand at 569.484 kb on - strand at 569.553 kb on - strand at 569.959 kb on + strand, within RR42_RS23350 at 569.959 kb on + strand, within RR42_RS23350 at 570.066 kb on + strand, within RR42_RS23350 at 570.066 kb on + strand, within RR42_RS23350 at 570.066 kb on + strand, within RR42_RS23350 at 570.067 kb on - strand, within RR42_RS23350 at 570.067 kb on - strand, within RR42_RS23350 at 570.274 kb on + strand at 570.472 kb on + strand, within RR42_RS23355 at 570.473 kb on - strand, within RR42_RS23355 at 570.682 kb on - strand at 570.871 kb on + strand, within RR42_RS23360 at 570.910 kb on - strand, within RR42_RS23360 at 571.134 kb on - strand, within RR42_RS23360 at 571.134 kb on - strand, within RR42_RS23360 at 571.145 kb on + strand, within RR42_RS23360 at 571.146 kb on - strand, within RR42_RS23360
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 569,435 + -0.6 569,435 + +1.5 569,466 + -0.5 569,467 - -0.7 569,467 - -0.7 569,467 - -2.6 569,467 - +0.9 569,484 - -0.2 569,553 - -0.0 569,959 + RR42_RS23350 0.56 -0.1 569,959 + RR42_RS23350 0.56 -1.7 570,066 + RR42_RS23350 0.70 -1.7 570,066 + RR42_RS23350 0.70 -0.4 570,066 + RR42_RS23350 0.70 +1.6 570,067 - RR42_RS23350 0.70 -2.2 570,067 - RR42_RS23350 0.70 +1.1 570,274 + -0.4 570,472 + RR42_RS23355 0.16 -0.6 570,473 - RR42_RS23355 0.16 -0.5 570,682 - -0.6 570,871 + RR42_RS23360 0.16 +0.9 570,910 - RR42_RS23360 0.21 -0.3 571,134 - RR42_RS23360 0.48 -0.3 571,134 - RR42_RS23360 0.48 -0.2 571,145 + RR42_RS23360 0.49 +0.1 571,146 - RR42_RS23360 0.49 -1.1
Or see this region's nucleotide sequence