Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22345 and RR42_RS22350 are separated by 150 nucleotides RR42_RS22350 and RR42_RS22355 are separated by 211 nucleotides RR42_RS22355 and RR42_RS22360 are separated by 263 nucleotides
RR42_RS22345: RR42_RS22345 - UDP-N-acetylmuramate--alanine ligase, at 354,042 to 354,395
_RS22345
RR42_RS22350: RR42_RS22350 - transporter, at 354,546 to 354,974
_RS22350
RR42_RS22355: RR42_RS22355 - ModE family transcriptional regulator, at 355,186 to 355,512
_RS22355
RR42_RS22360: RR42_RS22360 - hypothetical protein, at 355,776 to 355,985
_RS22360
Position (kb) 355
356 Strain fitness (log2 ratio) -2
-1
0
1
2 at 354.206 kb on + strand, within RR42_RS22345 at 354.207 kb on - strand, within RR42_RS22345 at 354.207 kb on - strand, within RR42_RS22345 at 354.318 kb on + strand, within RR42_RS22345 at 354.319 kb on - strand, within RR42_RS22345 at 354.381 kb on + strand at 354.382 kb on - strand at 354.458 kb on + strand at 354.459 kb on - strand at 354.578 kb on + strand at 355.025 kb on - strand at 355.041 kb on + strand at 355.041 kb on + strand at 355.041 kb on + strand at 355.041 kb on + strand at 355.042 kb on - strand at 355.042 kb on - strand at 355.042 kb on - strand at 355.071 kb on - strand at 355.112 kb on - strand at 355.432 kb on + strand, within RR42_RS22355 at 355.449 kb on - strand, within RR42_RS22355 at 355.524 kb on + strand at 355.525 kb on - strand at 355.643 kb on - strand at 355.643 kb on - strand at 355.807 kb on + strand, within RR42_RS22360 at 355.807 kb on + strand, within RR42_RS22360 at 355.975 kb on + strand at 355.976 kb on - strand at 356.006 kb on + strand at 356.006 kb on + strand at 356.007 kb on - strand at 356.087 kb on - strand at 356.218 kb on - strand at 356.218 kb on - strand at 356.501 kb on + strand at 356.501 kb on + strand at 356.502 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 354,206 + RR42_RS22345 0.46 -0.9 354,207 - RR42_RS22345 0.47 -2.2 354,207 - RR42_RS22345 0.47 +0.6 354,318 + RR42_RS22345 0.78 +0.5 354,319 - RR42_RS22345 0.78 +0.4 354,381 + +1.9 354,382 - +0.7 354,458 + -0.1 354,459 - -1.5 354,578 + -0.3 355,025 - +0.9 355,041 + -1.3 355,041 + -0.2 355,041 + -0.2 355,041 + +0.7 355,042 - -1.0 355,042 - -0.3 355,042 - -0.9 355,071 - -0.1 355,112 - +0.6 355,432 + RR42_RS22355 0.75 +1.7 355,449 - RR42_RS22355 0.80 -2.1 355,524 + +0.6 355,525 - -1.3 355,643 - -0.9 355,643 - +1.4 355,807 + RR42_RS22360 0.15 +0.4 355,807 + RR42_RS22360 0.15 -0.9 355,975 + -1.0 355,976 - +1.4 356,006 + +0.4 356,006 + +0.6 356,007 - +1.8 356,087 - -2.5 356,218 - -1.9 356,218 - -1.7 356,501 + +0.8 356,501 + -0.6 356,502 - +1.1
Or see this region's nucleotide sequence