Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22225 and RR42_RS22230 overlap by 4 nucleotides RR42_RS22230 and RR42_RS22235 are separated by 16 nucleotides RR42_RS22235 and RR42_RS22240 are separated by 821 nucleotides
RR42_RS22225: RR42_RS22225 - cytochrome C oxidase subunit III, at 324,981 to 325,388
_RS22225
RR42_RS22230: RR42_RS22230 - hypothetical protein, at 325,385 to 326,239
_RS22230
RR42_RS22235: RR42_RS22235 - peptidase M19, at 326,256 to 326,885
_RS22235
RR42_RS22240: RR42_RS22240 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase, at 327,707 to 329,008
_RS22240
Position (kb) 326
327 Strain fitness (log2 ratio) -2
-1
0
1 at 325.976 kb on + strand, within RR42_RS22230 at 326.082 kb on - strand, within RR42_RS22230 at 326.133 kb on - strand, within RR42_RS22230 at 326.173 kb on + strand at 326.371 kb on + strand, within RR42_RS22235 at 326.372 kb on - strand, within RR42_RS22235 at 326.659 kb on + strand, within RR42_RS22235 at 326.659 kb on + strand, within RR42_RS22235 at 326.659 kb on + strand, within RR42_RS22235 at 326.659 kb on + strand, within RR42_RS22235 at 326.660 kb on - strand, within RR42_RS22235 at 326.660 kb on - strand, within RR42_RS22235 at 326.827 kb on + strand at 326.828 kb on - strand at 326.828 kb on - strand at 327.005 kb on - strand at 327.651 kb on + strand at 327.652 kb on - strand at 327.652 kb on - strand at 327.697 kb on - strand at 327.751 kb on + strand at 327.752 kb on - strand at 327.752 kb on - strand at 327.752 kb on - strand at 327.755 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 325,976 + RR42_RS22230 0.69 -0.8 326,082 - RR42_RS22230 0.82 +0.9 326,133 - RR42_RS22230 0.87 -0.5 326,173 + -0.6 326,371 + RR42_RS22235 0.18 -0.4 326,372 - RR42_RS22235 0.18 +0.4 326,659 + RR42_RS22235 0.64 -1.5 326,659 + RR42_RS22235 0.64 -1.0 326,659 + RR42_RS22235 0.64 -0.1 326,659 + RR42_RS22235 0.64 -2.7 326,660 - RR42_RS22235 0.64 +1.2 326,660 - RR42_RS22235 0.64 -1.3 326,827 + +1.5 326,828 - +1.7 326,828 - +0.3 327,005 - +0.9 327,651 + -0.1 327,652 - +0.4 327,652 - -0.4 327,697 - -1.4 327,751 + +1.7 327,752 - +0.1 327,752 - -1.1 327,752 - -1.6 327,755 - -1.0
Or see this region's nucleotide sequence