Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22140 and RR42_RS22145 overlap by 4 nucleotides RR42_RS22145 and RR42_RS22150 are separated by 37 nucleotides RR42_RS22150 and RR42_RS22155 are separated by 7 nucleotides RR42_RS22155 and RR42_RS22160 overlap by 4 nucleotides
RR42_RS22140: RR42_RS22140 - cytochrome C, at 307,457 to 307,912
_RS22140
RR42_RS22145: RR42_RS22145 - amine dehydrogenase, at 307,909 to 308,430
_RS22145
RR42_RS22150: RR42_RS22150 - thiol-disulfide isomerase, at 308,468 to 309,055
_RS22150
RR42_RS22155: RR42_RS22155 - hypothetical protein, at 309,063 to 309,686
_RS22155
RR42_RS22160: RR42_RS22160 - amine dehydrogenase, at 309,683 to 310,828
_RS22160
Position (kb) 308
309
310 Strain fitness (log2 ratio) -2
-1
0
1 at 308.079 kb on - strand, within RR42_RS22145 at 308.090 kb on + strand, within RR42_RS22145 at 308.216 kb on + strand, within RR42_RS22145 at 308.216 kb on + strand, within RR42_RS22145 at 308.217 kb on - strand, within RR42_RS22145 at 308.510 kb on - strand at 308.655 kb on + strand, within RR42_RS22150 at 308.988 kb on + strand, within RR42_RS22150 at 308.988 kb on + strand, within RR42_RS22150 at 309.031 kb on + strand at 309.084 kb on - strand at 309.801 kb on + strand, within RR42_RS22160 at 309.801 kb on + strand, within RR42_RS22160 at 309.802 kb on - strand, within RR42_RS22160 at 309.802 kb on - strand, within RR42_RS22160 at 309.802 kb on - strand, within RR42_RS22160 at 309.802 kb on - strand at 309.957 kb on + strand, within RR42_RS22160 at 309.958 kb on - strand, within RR42_RS22160
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 308,079 - RR42_RS22145 0.33 -0.9 308,090 + RR42_RS22145 0.35 -0.8 308,216 + RR42_RS22145 0.59 +0.6 308,216 + RR42_RS22145 0.59 -0.1 308,217 - RR42_RS22145 0.59 -0.5 308,510 - -0.4 308,655 + RR42_RS22150 0.32 -1.0 308,988 + RR42_RS22150 0.88 -1.3 308,988 + RR42_RS22150 0.88 -0.3 309,031 + +0.3 309,084 - -1.0 309,801 + RR42_RS22160 0.10 -0.8 309,801 + RR42_RS22160 0.10 -1.8 309,802 - RR42_RS22160 0.10 -1.1 309,802 - RR42_RS22160 0.10 -0.1 309,802 - RR42_RS22160 0.10 -0.5 309,802 - +1.7 309,957 + RR42_RS22160 0.24 -1.5 309,958 - RR42_RS22160 0.24 -0.0
Or see this region's nucleotide sequence