Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS21865

Experiment: R2A with Vancomycin 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS21860 and RR42_RS21865 are separated by 155 nucleotidesRR42_RS21865 and RR42_RS21870 are separated by 165 nucleotides RR42_RS21860: RR42_RS21860 - hypothetical protein, at 230,852 to 231,661 _RS21860 RR42_RS21865: RR42_RS21865 - 3-ketoacyl-ACP reductase, at 231,817 to 232,560 _RS21865 RR42_RS21870: RR42_RS21870 - transcriptional regulator, at 232,726 to 233,607 _RS21870 Position (kb) 231 232 233Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 230.873 kb on + strandat 230.873 kb on + strandat 230.873 kb on + strandat 230.874 kb on - strandat 230.874 kb on - strandat 230.874 kb on - strandat 230.874 kb on - strandat 230.874 kb on - strandat 230.875 kb on + strandat 231.114 kb on + strand, within RR42_RS21860at 231.114 kb on + strand, within RR42_RS21860at 231.114 kb on + strand, within RR42_RS21860at 231.114 kb on + strand, within RR42_RS21860at 231.114 kb on + strand, within RR42_RS21860at 231.115 kb on - strand, within RR42_RS21860at 231.115 kb on - strand, within RR42_RS21860at 231.115 kb on - strand, within RR42_RS21860at 231.129 kb on + strand, within RR42_RS21860at 231.130 kb on - strand, within RR42_RS21860at 231.191 kb on + strand, within RR42_RS21860at 231.191 kb on + strand, within RR42_RS21860at 231.203 kb on - strand, within RR42_RS21860at 231.418 kb on - strand, within RR42_RS21860at 231.587 kb on + strandat 231.704 kb on + strandat 231.704 kb on + strandat 231.704 kb on + strandat 231.718 kb on + strandat 232.097 kb on + strand, within RR42_RS21865at 232.097 kb on + strand, within RR42_RS21865at 232.097 kb on + strand, within RR42_RS21865at 232.098 kb on - strand, within RR42_RS21865at 232.107 kb on - strand, within RR42_RS21865at 232.235 kb on + strand, within RR42_RS21865at 232.235 kb on + strand, within RR42_RS21865at 232.235 kb on + strand, within RR42_RS21865at 232.236 kb on - strand, within RR42_RS21865at 232.236 kb on - strand, within RR42_RS21865at 232.236 kb on - strand, within RR42_RS21865at 232.448 kb on + strandat 232.448 kb on + strand, within RR42_RS21865at 232.449 kb on - strand, within RR42_RS21865at 232.449 kb on - strand, within RR42_RS21865at 232.449 kb on - strand, within RR42_RS21865at 232.449 kb on - strand, within RR42_RS21865at 232.579 kb on + strandat 232.590 kb on + strandat 232.590 kb on + strandat 232.590 kb on + strandat 232.590 kb on + strandat 232.590 kb on + strandat 232.590 kb on + strandat 232.590 kb on + strandat 232.590 kb on + strandat 232.590 kb on + strandat 232.591 kb on - strandat 232.591 kb on - strandat 232.591 kb on - strandat 232.636 kb on - strandat 232.921 kb on + strand, within RR42_RS21870at 233.230 kb on + strand, within RR42_RS21870at 233.230 kb on + strand, within RR42_RS21870at 233.293 kb on + strand, within RR42_RS21870at 233.293 kb on + strand, within RR42_RS21870at 233.293 kb on + strand, within RR42_RS21870at 233.293 kb on + strand, within RR42_RS21870at 233.294 kb on - strand, within RR42_RS21870at 233.294 kb on - strand, within RR42_RS21870at 233.294 kb on - strand, within RR42_RS21870at 233.294 kb on - strand, within RR42_RS21870at 233.294 kb on - strand, within RR42_RS21870at 233.294 kb on - strand, within RR42_RS21870at 233.401 kb on + strand, within RR42_RS21870at 233.555 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml
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230,873 + +0.8
230,873 + -0.8
230,873 + -0.6
230,874 - +0.6
230,874 - -0.1
230,874 - -0.1
230,874 - +1.0
230,874 - +1.2
230,875 + -0.1
231,114 + RR42_RS21860 0.32 +0.6
231,114 + RR42_RS21860 0.32 +0.2
231,114 + RR42_RS21860 0.32 -0.1
231,114 + RR42_RS21860 0.32 -0.7
231,114 + RR42_RS21860 0.32 -0.2
231,115 - RR42_RS21860 0.32 +0.4
231,115 - RR42_RS21860 0.32 -0.2
231,115 - RR42_RS21860 0.32 +0.9
231,129 + RR42_RS21860 0.34 +0.0
231,130 - RR42_RS21860 0.34 -0.4
231,191 + RR42_RS21860 0.42 -3.5
231,191 + RR42_RS21860 0.42 +0.8
231,203 - RR42_RS21860 0.43 +0.2
231,418 - RR42_RS21860 0.70 +0.4
231,587 + -0.5
231,704 + +0.7
231,704 + -1.0
231,704 + -0.8
231,718 + +0.0
232,097 + RR42_RS21865 0.38 +0.3
232,097 + RR42_RS21865 0.38 -0.2
232,097 + RR42_RS21865 0.38 -1.4
232,098 - RR42_RS21865 0.38 -0.3
232,107 - RR42_RS21865 0.39 -1.1
232,235 + RR42_RS21865 0.56 -0.1
232,235 + RR42_RS21865 0.56 -0.7
232,235 + RR42_RS21865 0.56 -1.8
232,236 - RR42_RS21865 0.56 -0.1
232,236 - RR42_RS21865 0.56 -1.8
232,236 - RR42_RS21865 0.56 +0.5
232,448 + +1.7
232,448 + RR42_RS21865 0.85 -1.8
232,449 - RR42_RS21865 0.85 -1.7
232,449 - RR42_RS21865 0.85 -0.8
232,449 - RR42_RS21865 0.85 -1.4
232,449 - RR42_RS21865 0.85 +0.4
232,579 + +1.8
232,590 + +0.1
232,590 + -1.0
232,590 + -0.2
232,590 + -1.1
232,590 + +0.1
232,590 + +0.7
232,590 + +0.9
232,590 + -1.6
232,590 + -1.0
232,591 - -1.5
232,591 - +0.4
232,591 - -0.5
232,636 - +0.1
232,921 + RR42_RS21870 0.22 +1.2
233,230 + RR42_RS21870 0.57 +0.4
233,230 + RR42_RS21870 0.57 -0.5
233,293 + RR42_RS21870 0.64 +2.3
233,293 + RR42_RS21870 0.64 -1.1
233,293 + RR42_RS21870 0.64 +1.1
233,293 + RR42_RS21870 0.64 +0.4
233,294 - RR42_RS21870 0.64 -2.0
233,294 - RR42_RS21870 0.64 -0.1
233,294 - RR42_RS21870 0.64 -0.1
233,294 - RR42_RS21870 0.64 -0.2
233,294 - RR42_RS21870 0.64 -0.3
233,294 - RR42_RS21870 0.64 +0.7
233,401 + RR42_RS21870 0.77 -0.5
233,555 + -1.0

Or see this region's nucleotide sequence