Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21615 and RR42_RS21620 are separated by 20 nucleotides RR42_RS21620 and RR42_RS21625 are separated by 83 nucleotides RR42_RS21625 and RR42_RS21630 are separated by 381 nucleotides
RR42_RS21615: RR42_RS21615 - alcohol dehydrogenase, at 177,556 to 180,558
_RS21615
RR42_RS21620: RR42_RS21620 - ferredoxin, at 180,579 to 181,085
_RS21620
RR42_RS21625: RR42_RS21625 - 6-hydroxynicotinate 3-monooxygenase, at 181,169 to 182,323
_RS21625
RR42_RS21630: RR42_RS21630 - Asp/Glu racemase, at 182,705 to 183,457
_RS21630
Position (kb) 181
182
183 Strain fitness (log2 ratio) -2
-1
0
1
2 at 180.188 kb on + strand, within RR42_RS21615 at 180.388 kb on + strand at 180.388 kb on + strand at 180.388 kb on + strand at 180.388 kb on + strand at 180.389 kb on - strand at 180.797 kb on + strand, within RR42_RS21620 at 180.798 kb on - strand, within RR42_RS21620 at 180.958 kb on + strand, within RR42_RS21620 at 180.958 kb on + strand, within RR42_RS21620 at 180.998 kb on - strand, within RR42_RS21620 at 180.998 kb on - strand, within RR42_RS21620 at 180.998 kb on - strand, within RR42_RS21620 at 181.039 kb on + strand at 181.040 kb on - strand at 181.040 kb on - strand at 181.040 kb on - strand at 181.273 kb on + strand at 181.274 kb on - strand at 181.424 kb on - strand, within RR42_RS21625 at 181.424 kb on - strand, within RR42_RS21625 at 181.653 kb on + strand, within RR42_RS21625 at 181.654 kb on - strand, within RR42_RS21625 at 181.656 kb on + strand, within RR42_RS21625 at 181.657 kb on - strand, within RR42_RS21625 at 181.657 kb on - strand, within RR42_RS21625 at 181.687 kb on - strand, within RR42_RS21625 at 181.687 kb on - strand, within RR42_RS21625 at 181.687 kb on - strand, within RR42_RS21625 at 181.696 kb on + strand, within RR42_RS21625 at 181.697 kb on - strand, within RR42_RS21625 at 181.697 kb on - strand, within RR42_RS21625 at 181.697 kb on - strand, within RR42_RS21625 at 182.044 kb on - strand, within RR42_RS21625 at 182.226 kb on + strand at 182.227 kb on - strand at 182.500 kb on + strand at 182.500 kb on + strand at 182.500 kb on + strand at 182.501 kb on - strand at 182.501 kb on - strand at 182.501 kb on - strand at 182.975 kb on + strand, within RR42_RS21630 at 183.114 kb on - strand, within RR42_RS21630 at 183.116 kb on - strand, within RR42_RS21630
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 180,188 + RR42_RS21615 0.88 -0.1 180,388 + -1.0 180,388 + +1.1 180,388 + -1.4 180,388 + +1.0 180,389 - -0.8 180,797 + RR42_RS21620 0.43 -1.3 180,798 - RR42_RS21620 0.43 -0.8 180,958 + RR42_RS21620 0.75 +1.1 180,958 + RR42_RS21620 0.75 -0.2 180,998 - RR42_RS21620 0.83 +2.7 180,998 - RR42_RS21620 0.83 -0.0 180,998 - RR42_RS21620 0.83 -0.7 181,039 + -0.5 181,040 - +0.4 181,040 - -0.1 181,040 - -1.6 181,273 + +0.6 181,274 - -0.8 181,424 - RR42_RS21625 0.22 -0.9 181,424 - RR42_RS21625 0.22 +0.0 181,653 + RR42_RS21625 0.42 +0.7 181,654 - RR42_RS21625 0.42 -0.2 181,656 + RR42_RS21625 0.42 +0.6 181,657 - RR42_RS21625 0.42 -0.8 181,657 - RR42_RS21625 0.42 -0.3 181,687 - RR42_RS21625 0.45 +0.6 181,687 - RR42_RS21625 0.45 +0.6 181,687 - RR42_RS21625 0.45 -0.3 181,696 + RR42_RS21625 0.46 +1.1 181,697 - RR42_RS21625 0.46 -0.5 181,697 - RR42_RS21625 0.46 +0.5 181,697 - RR42_RS21625 0.46 -0.4 182,044 - RR42_RS21625 0.76 +1.4 182,226 + -0.1 182,227 - +0.9 182,500 + -0.3 182,500 + -1.1 182,500 + -1.5 182,501 - -2.1 182,501 - +2.0 182,501 - -0.4 182,975 + RR42_RS21630 0.36 -1.6 183,114 - RR42_RS21630 0.54 -1.6 183,116 - RR42_RS21630 0.55 +0.8
Or see this region's nucleotide sequence