Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21315 and RR42_RS21320 are separated by 8 nucleotides RR42_RS21320 and RR42_RS21325 are separated by 24 nucleotides RR42_RS21325 and RR42_RS21330 are separated by 101 nucleotides
RR42_RS21315: RR42_RS21315 - ethanolamine permease, at 108,691 to 110,139
_RS21315
RR42_RS21320: RR42_RS21320 - ethanolamine ammonia-lyase, at 110,148 to 111,530
_RS21320
RR42_RS21325: RR42_RS21325 - ethanolamine ammonia-lyase, at 111,555 to 112,367
_RS21325
RR42_RS21330: RR42_RS21330 - phosphoserine phosphatase, at 112,469 to 113,758
_RS21330
Position (kb) 110
111
112 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 109.149 kb on + strand, within RR42_RS21315 at 109.150 kb on - strand, within RR42_RS21315 at 109.557 kb on + strand, within RR42_RS21315 at 109.637 kb on - strand, within RR42_RS21315 at 109.765 kb on + strand, within RR42_RS21315 at 109.765 kb on + strand, within RR42_RS21315 at 109.765 kb on + strand, within RR42_RS21315 at 109.766 kb on - strand, within RR42_RS21315 at 109.766 kb on - strand, within RR42_RS21315 at 109.766 kb on - strand, within RR42_RS21315 at 109.766 kb on - strand, within RR42_RS21315 at 109.849 kb on + strand, within RR42_RS21315 at 109.850 kb on - strand, within RR42_RS21315 at 109.850 kb on - strand, within RR42_RS21315 at 109.850 kb on - strand, within RR42_RS21315 at 109.927 kb on + strand, within RR42_RS21315 at 110.014 kb on + strand at 110.014 kb on + strand at 110.015 kb on - strand at 110.015 kb on - strand at 110.048 kb on - strand at 110.048 kb on - strand at 110.048 kb on - strand at 110.241 kb on + strand at 110.242 kb on - strand at 111.003 kb on + strand, within RR42_RS21320 at 111.003 kb on + strand, within RR42_RS21320 at 111.003 kb on + strand, within RR42_RS21320 at 111.004 kb on - strand, within RR42_RS21320 at 111.150 kb on + strand, within RR42_RS21320 at 111.151 kb on - strand, within RR42_RS21320 at 111.151 kb on - strand, within RR42_RS21320 at 111.156 kb on + strand, within RR42_RS21320 at 111.157 kb on - strand, within RR42_RS21320 at 111.275 kb on + strand, within RR42_RS21320 at 111.276 kb on - strand, within RR42_RS21320 at 111.360 kb on + strand, within RR42_RS21320 at 111.391 kb on - strand, within RR42_RS21320 at 111.407 kb on - strand at 111.529 kb on - strand at 111.801 kb on + strand, within RR42_RS21325 at 111.801 kb on + strand, within RR42_RS21325 at 111.802 kb on - strand, within RR42_RS21325 at 112.122 kb on + strand, within RR42_RS21325 at 112.184 kb on - strand, within RR42_RS21325 at 112.352 kb on + strand at 112.495 kb on - strand at 112.495 kb on - strand at 112.495 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 109,149 + RR42_RS21315 0.32 +1.4 109,150 - RR42_RS21315 0.32 +0.6 109,557 + RR42_RS21315 0.60 +0.6 109,637 - RR42_RS21315 0.65 +0.4 109,765 + RR42_RS21315 0.74 -1.8 109,765 + RR42_RS21315 0.74 -0.2 109,765 + RR42_RS21315 0.74 -0.6 109,766 - RR42_RS21315 0.74 +0.1 109,766 - RR42_RS21315 0.74 -0.1 109,766 - RR42_RS21315 0.74 -0.2 109,766 - RR42_RS21315 0.74 -0.6 109,849 + RR42_RS21315 0.80 -0.2 109,850 - RR42_RS21315 0.80 -1.4 109,850 - RR42_RS21315 0.80 -0.1 109,850 - RR42_RS21315 0.80 +0.1 109,927 + RR42_RS21315 0.85 +0.6 110,014 + -0.9 110,014 + +0.5 110,015 - -1.0 110,015 - +0.1 110,048 - -1.2 110,048 - +0.1 110,048 - +0.8 110,241 + -1.6 110,242 - +0.7 111,003 + RR42_RS21320 0.62 +2.5 111,003 + RR42_RS21320 0.62 -0.7 111,003 + RR42_RS21320 0.62 -0.5 111,004 - RR42_RS21320 0.62 +0.1 111,150 + RR42_RS21320 0.72 -0.9 111,151 - RR42_RS21320 0.73 -1.6 111,151 - RR42_RS21320 0.73 +5.6 111,156 + RR42_RS21320 0.73 -1.2 111,157 - RR42_RS21320 0.73 -1.8 111,275 + RR42_RS21320 0.81 -1.4 111,276 - RR42_RS21320 0.82 -0.3 111,360 + RR42_RS21320 0.88 +0.3 111,391 - RR42_RS21320 0.90 +0.8 111,407 - +0.9 111,529 - -0.4 111,801 + RR42_RS21325 0.30 +0.1 111,801 + RR42_RS21325 0.30 -0.1 111,802 - RR42_RS21325 0.30 -0.6 112,122 + RR42_RS21325 0.70 +1.2 112,184 - RR42_RS21325 0.77 +0.1 112,352 + +0.6 112,495 - -0.0 112,495 - +0.4 112,495 - +0.3
Or see this region's nucleotide sequence