Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21170 and RR42_RS21175 are separated by 31 nucleotides RR42_RS21175 and RR42_RS21180 are separated by 54 nucleotides RR42_RS21180 and RR42_RS21185 are separated by 172 nucleotides
RR42_RS21170: RR42_RS21170 - MBL fold metallo-hydrolase, at 75,003 to 76,004
_RS21170
RR42_RS21175: RR42_RS21175 - transporter, at 76,036 to 76,470
_RS21175
RR42_RS21180: RR42_RS21180 - GNAT family acetyltransferase, at 76,525 to 76,914
_RS21180
RR42_RS21185: RR42_RS21185 - AMP nucleosidase, at 77,087 to 78,583
_RS21185
Position (kb) 76
77 Strain fitness (log2 ratio) -2
-1
0
1 at 75.541 kb on - strand, within RR42_RS21170 at 75.726 kb on + strand, within RR42_RS21170 at 75.726 kb on + strand, within RR42_RS21170 at 75.727 kb on - strand, within RR42_RS21170 at 75.957 kb on + strand at 76.002 kb on + strand at 76.006 kb on - strand at 76.006 kb on - strand at 76.006 kb on - strand at 76.051 kb on + strand at 76.052 kb on - strand at 76.211 kb on + strand, within RR42_RS21175 at 76.211 kb on + strand, within RR42_RS21175 at 76.211 kb on + strand, within RR42_RS21175 at 76.212 kb on - strand, within RR42_RS21175 at 76.715 kb on + strand, within RR42_RS21180 at 76.938 kb on - strand at 77.020 kb on + strand at 77.020 kb on + strand at 77.083 kb on + strand at 77.189 kb on + strand at 77.273 kb on + strand, within RR42_RS21185 at 77.360 kb on + strand, within RR42_RS21185 at 77.360 kb on + strand, within RR42_RS21185 at 77.400 kb on - strand, within RR42_RS21185 at 77.402 kb on + strand, within RR42_RS21185 at 77.428 kb on - strand, within RR42_RS21185 at 77.519 kb on + strand, within RR42_RS21185 at 77.519 kb on + strand, within RR42_RS21185 at 77.520 kb on - strand, within RR42_RS21185 at 77.520 kb on - strand, within RR42_RS21185 at 77.600 kb on + strand, within RR42_RS21185 at 77.600 kb on + strand, within RR42_RS21185 at 77.601 kb on - strand, within RR42_RS21185 at 77.601 kb on - strand, within RR42_RS21185 at 77.679 kb on - strand, within RR42_RS21185 at 77.679 kb on - strand, within RR42_RS21185 at 77.679 kb on - strand, within RR42_RS21185 at 77.685 kb on - strand, within RR42_RS21185 at 77.685 kb on - strand, within RR42_RS21185 at 77.735 kb on + strand, within RR42_RS21185 at 77.735 kb on + strand, within RR42_RS21185 at 77.735 kb on + strand, within RR42_RS21185 at 77.744 kb on + strand, within RR42_RS21185 at 77.814 kb on - strand, within RR42_RS21185 at 77.912 kb on + strand, within RR42_RS21185 at 77.913 kb on - strand, within RR42_RS21185
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 75,541 - RR42_RS21170 0.54 -0.8 75,726 + RR42_RS21170 0.72 -0.6 75,726 + RR42_RS21170 0.72 -1.2 75,727 - RR42_RS21170 0.72 +0.2 75,957 + -0.3 76,002 + -0.9 76,006 - -0.5 76,006 - -0.4 76,006 - +0.4 76,051 + +1.1 76,052 - +0.4 76,211 + RR42_RS21175 0.40 +0.1 76,211 + RR42_RS21175 0.40 -0.3 76,211 + RR42_RS21175 0.40 -0.4 76,212 - RR42_RS21175 0.40 -0.1 76,715 + RR42_RS21180 0.49 -1.1 76,938 - +1.1 77,020 + -2.2 77,020 + +0.1 77,083 + +0.5 77,189 + +1.4 77,273 + RR42_RS21185 0.12 -0.8 77,360 + RR42_RS21185 0.18 -0.4 77,360 + RR42_RS21185 0.18 +0.0 77,400 - RR42_RS21185 0.21 +0.2 77,402 + RR42_RS21185 0.21 -0.1 77,428 - RR42_RS21185 0.23 -0.6 77,519 + RR42_RS21185 0.29 -0.1 77,519 + RR42_RS21185 0.29 -0.9 77,520 - RR42_RS21185 0.29 +0.2 77,520 - RR42_RS21185 0.29 -0.1 77,600 + RR42_RS21185 0.34 -0.6 77,600 + RR42_RS21185 0.34 +0.8 77,601 - RR42_RS21185 0.34 +0.5 77,601 - RR42_RS21185 0.34 +0.3 77,679 - RR42_RS21185 0.40 -0.3 77,679 - RR42_RS21185 0.40 +0.1 77,679 - RR42_RS21185 0.40 +0.5 77,685 - RR42_RS21185 0.40 -1.0 77,685 - RR42_RS21185 0.40 -0.8 77,735 + RR42_RS21185 0.43 -2.1 77,735 + RR42_RS21185 0.43 +1.1 77,735 + RR42_RS21185 0.43 -1.2 77,744 + RR42_RS21185 0.44 +0.5 77,814 - RR42_RS21185 0.49 +1.1 77,912 + RR42_RS21185 0.55 -0.3 77,913 - RR42_RS21185 0.55 -0.4
Or see this region's nucleotide sequence