Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS19485 and RR42_RS19490 are separated by 44 nucleotides RR42_RS19490 and RR42_RS19495 are separated by 58 nucleotides RR42_RS19495 and RR42_RS19500 are separated by 20 nucleotides
RR42_RS19485: RR42_RS19485 - general secretion pathway protein GspH, at 4,207,686 to 4,208,180
_RS19485
RR42_RS19490: RR42_RS19490 - general secretion pathway protein GspI, at 4,208,225 to 4,208,605
_RS19490
RR42_RS19495: RR42_RS19495 - general secretion pathway protein GspJ, at 4,208,664 to 4,209,335
_RS19495
RR42_RS19500: RR42_RS19500 - general secretion pathway protein GspK, at 4,209,356 to 4,210,351
_RS19500
Position (kb)
4208
4209
4210 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7 at 4207.796 kb on - strand, within RR42_RS19485 at 4208.160 kb on + strand at 4208.161 kb on - strand at 4208.212 kb on + strand at 4208.212 kb on + strand at 4208.213 kb on - strand at 4208.463 kb on - strand, within RR42_RS19490 at 4208.463 kb on - strand, within RR42_RS19490 at 4208.463 kb on - strand, within RR42_RS19490 at 4208.612 kb on + strand at 4208.622 kb on - strand at 4208.630 kb on + strand at 4208.630 kb on + strand at 4208.630 kb on + strand at 4208.630 kb on + strand at 4208.630 kb on + strand at 4208.631 kb on - strand at 4208.631 kb on - strand at 4208.812 kb on + strand, within RR42_RS19495 at 4208.812 kb on + strand, within RR42_RS19495 at 4208.813 kb on - strand, within RR42_RS19495 at 4208.813 kb on - strand, within RR42_RS19495 at 4208.813 kb on - strand, within RR42_RS19495 at 4208.813 kb on - strand, within RR42_RS19495 at 4208.814 kb on + strand, within RR42_RS19495 at 4208.814 kb on + strand, within RR42_RS19495 at 4208.815 kb on - strand, within RR42_RS19495 at 4208.815 kb on - strand, within RR42_RS19495 at 4208.815 kb on - strand, within RR42_RS19495 at 4208.958 kb on + strand, within RR42_RS19495 at 4209.331 kb on + strand at 4209.331 kb on + strand at 4209.459 kb on + strand, within RR42_RS19500 at 4209.459 kb on + strand, within RR42_RS19500 at 4209.862 kb on - strand, within RR42_RS19500 at 4210.195 kb on - strand, within RR42_RS19500 at 4210.195 kb on - strand, within RR42_RS19500 at 4210.205 kb on + strand, within RR42_RS19500 at 4210.206 kb on - strand, within RR42_RS19500 at 4210.206 kb on - strand, within RR42_RS19500 at 4210.206 kb on - strand, within RR42_RS19500 at 4210.217 kb on + strand, within RR42_RS19500 at 4210.218 kb on - strand, within RR42_RS19500 at 4210.218 kb on - strand, within RR42_RS19500 at 4210.269 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 4,207,796 - RR42_RS19485 0.22 +6.3 4,208,160 + -0.2 4,208,161 - +4.6 4,208,212 + -0.2 4,208,212 + +0.0 4,208,213 - +6.2 4,208,463 - RR42_RS19490 0.62 +6.9 4,208,463 - RR42_RS19490 0.62 +5.1 4,208,463 - RR42_RS19490 0.62 +6.2 4,208,612 + -0.4 4,208,622 - +7.0 4,208,630 + -1.2 4,208,630 + +3.7 4,208,630 + +0.4 4,208,630 + +6.4 4,208,630 + -1.8 4,208,631 - +6.6 4,208,631 - +6.4 4,208,812 + RR42_RS19495 0.22 +7.4 4,208,812 + RR42_RS19495 0.22 +5.2 4,208,813 - RR42_RS19495 0.22 +6.2 4,208,813 - RR42_RS19495 0.22 +6.2 4,208,813 - RR42_RS19495 0.22 +6.9 4,208,813 - RR42_RS19495 0.22 +6.4 4,208,814 + RR42_RS19495 0.22 +4.0 4,208,814 + RR42_RS19495 0.22 +6.5 4,208,815 - RR42_RS19495 0.22 +5.7 4,208,815 - RR42_RS19495 0.22 +6.8 4,208,815 - RR42_RS19495 0.22 +7.0 4,208,958 + RR42_RS19495 0.44 +7.0 4,209,331 + -0.3 4,209,331 + +1.4 4,209,459 + RR42_RS19500 0.10 +6.5 4,209,459 + RR42_RS19500 0.10 +5.7 4,209,862 - RR42_RS19500 0.51 +6.4 4,210,195 - RR42_RS19500 0.84 +5.8 4,210,195 - RR42_RS19500 0.84 +7.0 4,210,205 + RR42_RS19500 0.85 +6.1 4,210,206 - RR42_RS19500 0.85 +6.3 4,210,206 - RR42_RS19500 0.85 +7.6 4,210,206 - RR42_RS19500 0.85 +6.9 4,210,217 + RR42_RS19500 0.86 +6.2 4,210,218 - RR42_RS19500 0.87 +6.6 4,210,218 - RR42_RS19500 0.87 +6.0 4,210,269 - +5.8
Or see this region's nucleotide sequence