Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS19475 and RR42_RS19480 are separated by 285 nucleotides RR42_RS19480 and RR42_RS19485 are separated by 36 nucleotides RR42_RS19485 and RR42_RS19490 are separated by 44 nucleotides RR42_RS19490 and RR42_RS19495 are separated by 58 nucleotides
RR42_RS19475: RR42_RS19475 - general secretion pathway protein, at 4,206,297 to 4,206,896
_RS19475
RR42_RS19480: RR42_RS19480 - secretion system protein G, at 4,207,182 to 4,207,649
_RS19480
RR42_RS19485: RR42_RS19485 - general secretion pathway protein GspH, at 4,207,686 to 4,208,180
_RS19485
RR42_RS19490: RR42_RS19490 - general secretion pathway protein GspI, at 4,208,225 to 4,208,605
_RS19490
RR42_RS19495: RR42_RS19495 - general secretion pathway protein GspJ, at 4,208,664 to 4,209,335
_RS19495
Position (kb) 4207
4208
4209 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7 at 4206.819 kb on + strand, within RR42_RS19475 at 4206.819 kb on + strand, within RR42_RS19475 at 4206.922 kb on + strand at 4206.922 kb on + strand at 4206.922 kb on + strand at 4206.922 kb on + strand at 4206.922 kb on + strand at 4206.944 kb on - strand at 4206.944 kb on - strand at 4206.944 kb on - strand at 4207.093 kb on + strand at 4207.094 kb on - strand at 4207.095 kb on + strand at 4207.095 kb on + strand at 4207.095 kb on + strand at 4207.096 kb on - strand at 4207.096 kb on - strand at 4207.140 kb on - strand at 4207.141 kb on + strand at 4207.141 kb on + strand at 4207.141 kb on + strand at 4207.141 kb on + strand at 4207.141 kb on + strand at 4207.141 kb on + strand at 4207.141 kb on + strand at 4207.142 kb on - strand at 4207.142 kb on - strand at 4207.142 kb on - strand at 4207.162 kb on - strand at 4207.168 kb on + strand at 4207.344 kb on - strand, within RR42_RS19480 at 4207.395 kb on + strand, within RR42_RS19480 at 4207.395 kb on + strand, within RR42_RS19480 at 4207.481 kb on + strand, within RR42_RS19480 at 4207.482 kb on - strand, within RR42_RS19480 at 4207.482 kb on - strand, within RR42_RS19480 at 4207.500 kb on + strand, within RR42_RS19480 at 4207.501 kb on - strand, within RR42_RS19480 at 4207.539 kb on + strand, within RR42_RS19480 at 4207.540 kb on - strand, within RR42_RS19480 at 4207.540 kb on - strand, within RR42_RS19480 at 4207.545 kb on + strand, within RR42_RS19480 at 4207.546 kb on - strand, within RR42_RS19480 at 4207.546 kb on - strand, within RR42_RS19480 at 4207.546 kb on - strand, within RR42_RS19480 at 4207.647 kb on + strand at 4207.647 kb on + strand at 4207.647 kb on + strand at 4207.796 kb on - strand, within RR42_RS19485 at 4208.160 kb on + strand at 4208.161 kb on - strand at 4208.212 kb on + strand at 4208.212 kb on + strand at 4208.213 kb on - strand at 4208.463 kb on - strand, within RR42_RS19490 at 4208.463 kb on - strand, within RR42_RS19490 at 4208.463 kb on - strand, within RR42_RS19490 at 4208.612 kb on + strand at 4208.622 kb on - strand at 4208.630 kb on + strand at 4208.630 kb on + strand at 4208.630 kb on + strand at 4208.630 kb on + strand at 4208.630 kb on + strand at 4208.631 kb on - strand at 4208.631 kb on - strand at 4208.812 kb on + strand, within RR42_RS19495 at 4208.812 kb on + strand, within RR42_RS19495 at 4208.813 kb on - strand, within RR42_RS19495 at 4208.813 kb on - strand, within RR42_RS19495 at 4208.813 kb on - strand, within RR42_RS19495 at 4208.813 kb on - strand, within RR42_RS19495 at 4208.814 kb on + strand, within RR42_RS19495 at 4208.814 kb on + strand, within RR42_RS19495 at 4208.815 kb on - strand, within RR42_RS19495 at 4208.815 kb on - strand, within RR42_RS19495 at 4208.815 kb on - strand, within RR42_RS19495 at 4208.958 kb on + strand, within RR42_RS19495
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 4,206,819 + RR42_RS19475 0.87 +6.2 4,206,819 + RR42_RS19475 0.87 +6.6 4,206,922 + +5.7 4,206,922 + +5.0 4,206,922 + +6.4 4,206,922 + +6.7 4,206,922 + +6.1 4,206,944 - +0.0 4,206,944 - -0.3 4,206,944 - +0.6 4,207,093 + +1.8 4,207,094 - +0.1 4,207,095 + +0.4 4,207,095 + +0.1 4,207,095 + -0.3 4,207,096 - +5.9 4,207,096 - +6.4 4,207,140 - +5.9 4,207,141 + +0.2 4,207,141 + -0.1 4,207,141 + +0.4 4,207,141 + -0.1 4,207,141 + -0.6 4,207,141 + -0.0 4,207,141 + +1.4 4,207,142 - +6.0 4,207,142 - +6.0 4,207,142 - +6.2 4,207,162 - +6.3 4,207,168 + -1.0 4,207,344 - RR42_RS19480 0.35 +6.0 4,207,395 + RR42_RS19480 0.46 +6.2 4,207,395 + RR42_RS19480 0.46 +6.2 4,207,481 + RR42_RS19480 0.64 +6.5 4,207,482 - RR42_RS19480 0.64 +6.1 4,207,482 - RR42_RS19480 0.64 +5.7 4,207,500 + RR42_RS19480 0.68 +7.2 4,207,501 - RR42_RS19480 0.68 +5.5 4,207,539 + RR42_RS19480 0.76 +6.1 4,207,540 - RR42_RS19480 0.76 +6.4 4,207,540 - RR42_RS19480 0.76 +6.4 4,207,545 + RR42_RS19480 0.78 +5.4 4,207,546 - RR42_RS19480 0.78 +2.1 4,207,546 - RR42_RS19480 0.78 +6.4 4,207,546 - RR42_RS19480 0.78 +5.8 4,207,647 + -0.7 4,207,647 + -1.0 4,207,647 + -0.6 4,207,796 - RR42_RS19485 0.22 +6.3 4,208,160 + -0.2 4,208,161 - +4.6 4,208,212 + -0.2 4,208,212 + +0.0 4,208,213 - +6.2 4,208,463 - RR42_RS19490 0.62 +6.9 4,208,463 - RR42_RS19490 0.62 +5.1 4,208,463 - RR42_RS19490 0.62 +6.2 4,208,612 + -0.4 4,208,622 - +7.0 4,208,630 + -1.2 4,208,630 + +3.7 4,208,630 + +0.4 4,208,630 + +6.4 4,208,630 + -1.8 4,208,631 - +6.6 4,208,631 - +6.4 4,208,812 + RR42_RS19495 0.22 +7.4 4,208,812 + RR42_RS19495 0.22 +5.2 4,208,813 - RR42_RS19495 0.22 +6.2 4,208,813 - RR42_RS19495 0.22 +6.2 4,208,813 - RR42_RS19495 0.22 +6.9 4,208,813 - RR42_RS19495 0.22 +6.4 4,208,814 + RR42_RS19495 0.22 +4.0 4,208,814 + RR42_RS19495 0.22 +6.5 4,208,815 - RR42_RS19495 0.22 +5.7 4,208,815 - RR42_RS19495 0.22 +6.8 4,208,815 - RR42_RS19495 0.22 +7.0 4,208,958 + RR42_RS19495 0.44 +7.0
Or see this region's nucleotide sequence