Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS18360 and RR42_RS18365 are separated by 333 nucleotides RR42_RS18365 and RR42_RS18370 are separated by 222 nucleotides
RR42_RS18360: RR42_RS18360 - ubiquinone biosynthesis protein, at 3,978,902 to 3,980,173
_RS18360
RR42_RS18365: RR42_RS18365 - GTP-binding protein, at 3,980,507 to 3,981,598
_RS18365
RR42_RS18370: RR42_RS18370 - integrase, at 3,981,821 to 3,983,089
_RS18370
Position (kb)
3980
3981
3982 Strain fitness (log2 ratio)
-1
0
1
2 at 3980.184 kb on + strand at 3980.185 kb on - strand at 3980.185 kb on - strand at 3980.185 kb on - strand at 3980.185 kb on - strand at 3980.334 kb on + strand at 3980.335 kb on - strand at 3980.600 kb on + strand at 3980.601 kb on - strand at 3981.117 kb on - strand, within RR42_RS18365 at 3981.117 kb on - strand, within RR42_RS18365 at 3981.350 kb on + strand, within RR42_RS18365 at 3981.351 kb on - strand, within RR42_RS18365 at 3981.476 kb on + strand, within RR42_RS18365 at 3981.490 kb on + strand at 3981.490 kb on + strand at 3981.491 kb on - strand at 3981.491 kb on - strand at 3981.494 kb on + strand at 3981.495 kb on - strand at 3981.495 kb on - strand at 3981.495 kb on - strand at 3981.552 kb on - strand at 3981.552 kb on - strand at 3981.661 kb on + strand at 3981.685 kb on + strand at 3981.686 kb on - strand at 3981.734 kb on + strand at 3981.870 kb on - strand at 3981.870 kb on - strand at 3981.896 kb on + strand at 3981.897 kb on - strand at 3981.961 kb on + strand, within RR42_RS18370 at 3981.961 kb on + strand, within RR42_RS18370 at 3981.961 kb on + strand, within RR42_RS18370 at 3981.961 kb on + strand, within RR42_RS18370 at 3981.962 kb on - strand, within RR42_RS18370 at 3981.962 kb on - strand, within RR42_RS18370 at 3981.978 kb on + strand, within RR42_RS18370 at 3981.979 kb on - strand, within RR42_RS18370 at 3981.979 kb on - strand, within RR42_RS18370 at 3982.061 kb on - strand, within RR42_RS18370 at 3982.061 kb on - strand, within RR42_RS18370 at 3982.212 kb on + strand, within RR42_RS18370 at 3982.231 kb on + strand, within RR42_RS18370 at 3982.231 kb on + strand, within RR42_RS18370 at 3982.232 kb on - strand, within RR42_RS18370 at 3982.232 kb on - strand, within RR42_RS18370 at 3982.257 kb on + strand, within RR42_RS18370 at 3982.258 kb on - strand, within RR42_RS18370 at 3982.258 kb on - strand, within RR42_RS18370 at 3982.258 kb on - strand, within RR42_RS18370 at 3982.329 kb on + strand, within RR42_RS18370 at 3982.329 kb on + strand, within RR42_RS18370 at 3982.330 kb on - strand, within RR42_RS18370 at 3982.541 kb on + strand, within RR42_RS18370 at 3982.542 kb on - strand, within RR42_RS18370
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,980,184 + -0.3 3,980,185 - +1.4 3,980,185 - -1.3 3,980,185 - +0.8 3,980,185 - +2.0 3,980,334 + -0.7 3,980,335 - +0.5 3,980,600 + +1.0 3,980,601 - -1.0 3,981,117 - RR42_RS18365 0.56 +0.0 3,981,117 - RR42_RS18365 0.56 -0.6 3,981,350 + RR42_RS18365 0.77 -1.2 3,981,351 - RR42_RS18365 0.77 -0.4 3,981,476 + RR42_RS18365 0.89 -0.9 3,981,490 + -1.3 3,981,490 + -0.6 3,981,491 - +1.0 3,981,491 - +0.3 3,981,494 + -0.9 3,981,495 - -0.9 3,981,495 - -1.0 3,981,495 - -1.2 3,981,552 - -0.9 3,981,552 - -1.4 3,981,661 + -1.2 3,981,685 + -0.0 3,981,686 - -0.2 3,981,734 + -0.6 3,981,870 - -0.1 3,981,870 - +0.3 3,981,896 + -0.2 3,981,897 - +0.1 3,981,961 + RR42_RS18370 0.11 -0.2 3,981,961 + RR42_RS18370 0.11 -1.5 3,981,961 + RR42_RS18370 0.11 -1.5 3,981,961 + RR42_RS18370 0.11 -0.8 3,981,962 - RR42_RS18370 0.11 -0.7 3,981,962 - RR42_RS18370 0.11 +0.4 3,981,978 + RR42_RS18370 0.12 -0.1 3,981,979 - RR42_RS18370 0.12 +1.3 3,981,979 - RR42_RS18370 0.12 -0.5 3,982,061 - RR42_RS18370 0.19 -0.2 3,982,061 - RR42_RS18370 0.19 +0.6 3,982,212 + RR42_RS18370 0.31 -0.5 3,982,231 + RR42_RS18370 0.32 -0.2 3,982,231 + RR42_RS18370 0.32 +2.0 3,982,232 - RR42_RS18370 0.32 -1.2 3,982,232 - RR42_RS18370 0.32 -0.3 3,982,257 + RR42_RS18370 0.34 +0.4 3,982,258 - RR42_RS18370 0.34 -0.5 3,982,258 - RR42_RS18370 0.34 -1.3 3,982,258 - RR42_RS18370 0.34 -1.3 3,982,329 + RR42_RS18370 0.40 +1.2 3,982,329 + RR42_RS18370 0.40 +1.3 3,982,330 - RR42_RS18370 0.40 -0.6 3,982,541 + RR42_RS18370 0.57 -0.6 3,982,542 - RR42_RS18370 0.57 -1.5
Or see this region's nucleotide sequence