Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS17915 and RR42_RS17920 overlap by 4 nucleotides RR42_RS17920 and RR42_RS17925 are separated by 23 nucleotides RR42_RS17925 and RR42_RS17930 are separated by 185 nucleotides RR42_RS17930 and RR42_RS17935 overlap by 8 nucleotides
RR42_RS17915: RR42_RS17915 - proline dioxygenase, at 3,876,751 to 3,877,701
_RS17915
RR42_RS17920: RR42_RS17920 - lytic transglycosylase, at 3,877,698 to 3,878,444
_RS17920
RR42_RS17925: RR42_RS17925 - prolyl-tRNA synthetase, at 3,878,468 to 3,880,189
_RS17925
RR42_RS17930: RR42_RS17930 - RNA pyrophosphohydrolase, at 3,880,375 to 3,881,037
_RS17930
RR42_RS17935: RR42_RS17935 - lipoprotein, at 3,881,030 to 3,881,659
_RS17935
Position (kb)
3878
3879
3880
3881 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6 at 3877.655 kb on + strand at 3877.865 kb on - strand, within RR42_RS17920 at 3877.891 kb on + strand, within RR42_RS17920 at 3877.892 kb on - strand, within RR42_RS17920 at 3877.936 kb on + strand, within RR42_RS17920 at 3877.936 kb on + strand, within RR42_RS17920 at 3877.936 kb on + strand, within RR42_RS17920 at 3877.936 kb on + strand, within RR42_RS17920 at 3877.937 kb on - strand, within RR42_RS17920 at 3877.937 kb on - strand, within RR42_RS17920 at 3877.960 kb on + strand, within RR42_RS17920 at 3878.034 kb on + strand, within RR42_RS17920 at 3878.034 kb on + strand, within RR42_RS17920 at 3878.034 kb on + strand, within RR42_RS17920 at 3878.035 kb on - strand, within RR42_RS17920 at 3878.035 kb on - strand, within RR42_RS17920 at 3878.035 kb on - strand, within RR42_RS17920 at 3878.035 kb on - strand, within RR42_RS17920 at 3878.035 kb on - strand, within RR42_RS17920 at 3878.075 kb on - strand, within RR42_RS17920 at 3878.075 kb on - strand, within RR42_RS17920 at 3878.075 kb on - strand, within RR42_RS17920 at 3878.149 kb on + strand at 3878.149 kb on + strand, within RR42_RS17920 at 3878.149 kb on + strand, within RR42_RS17920 at 3878.149 kb on + strand, within RR42_RS17920 at 3878.150 kb on - strand, within RR42_RS17920 at 3878.150 kb on - strand, within RR42_RS17920 at 3878.150 kb on - strand, within RR42_RS17920 at 3878.152 kb on + strand, within RR42_RS17920 at 3878.152 kb on + strand, within RR42_RS17920 at 3878.239 kb on + strand, within RR42_RS17920 at 3880.338 kb on - strand at 3880.338 kb on - strand at 3880.338 kb on - strand at 3880.535 kb on + strand, within RR42_RS17930 at 3880.536 kb on - strand, within RR42_RS17930 at 3880.536 kb on - strand, within RR42_RS17930 at 3880.536 kb on - strand, within RR42_RS17930 at 3880.649 kb on - strand, within RR42_RS17930 at 3880.759 kb on + strand, within RR42_RS17930 at 3880.759 kb on + strand, within RR42_RS17930 at 3880.805 kb on - strand, within RR42_RS17930 at 3880.848 kb on + strand, within RR42_RS17930 at 3880.909 kb on + strand, within RR42_RS17930 at 3881.037 kb on - strand at 3881.171 kb on + strand, within RR42_RS17935 at 3881.172 kb on - strand, within RR42_RS17935
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,877,655 + -0.7 3,877,865 - RR42_RS17920 0.22 +4.7 3,877,891 + RR42_RS17920 0.26 +4.3 3,877,892 - RR42_RS17920 0.26 +4.7 3,877,936 + RR42_RS17920 0.32 +4.4 3,877,936 + RR42_RS17920 0.32 +4.8 3,877,936 + RR42_RS17920 0.32 +4.7 3,877,936 + RR42_RS17920 0.32 +4.2 3,877,937 - RR42_RS17920 0.32 +4.1 3,877,937 - RR42_RS17920 0.32 +4.3 3,877,960 + RR42_RS17920 0.35 +4.7 3,878,034 + RR42_RS17920 0.45 +4.2 3,878,034 + RR42_RS17920 0.45 +4.4 3,878,034 + RR42_RS17920 0.45 +4.1 3,878,035 - RR42_RS17920 0.45 +4.4 3,878,035 - RR42_RS17920 0.45 +3.7 3,878,035 - RR42_RS17920 0.45 +4.5 3,878,035 - RR42_RS17920 0.45 +5.3 3,878,035 - RR42_RS17920 0.45 +3.7 3,878,075 - RR42_RS17920 0.50 +6.2 3,878,075 - RR42_RS17920 0.50 +4.5 3,878,075 - RR42_RS17920 0.50 +4.6 3,878,149 + +4.6 3,878,149 + RR42_RS17920 0.60 +6.2 3,878,149 + RR42_RS17920 0.60 +3.5 3,878,149 + RR42_RS17920 0.60 +4.3 3,878,150 - RR42_RS17920 0.61 +5.1 3,878,150 - RR42_RS17920 0.61 +4.1 3,878,150 - RR42_RS17920 0.61 +4.2 3,878,152 + RR42_RS17920 0.61 +4.3 3,878,152 + RR42_RS17920 0.61 +3.9 3,878,239 + RR42_RS17920 0.72 +4.6 3,880,338 - -1.7 3,880,338 - +0.0 3,880,338 - -1.8 3,880,535 + RR42_RS17930 0.24 +0.2 3,880,536 - RR42_RS17930 0.24 -1.1 3,880,536 - RR42_RS17930 0.24 -0.2 3,880,536 - RR42_RS17930 0.24 -1.2 3,880,649 - RR42_RS17930 0.41 -0.9 3,880,759 + RR42_RS17930 0.58 +0.2 3,880,759 + RR42_RS17930 0.58 -0.9 3,880,805 - RR42_RS17930 0.65 -0.7 3,880,848 + RR42_RS17930 0.71 -0.4 3,880,909 + RR42_RS17930 0.81 +0.8 3,881,037 - +0.3 3,881,171 + RR42_RS17935 0.22 +0.1 3,881,172 - RR42_RS17935 0.23 -1.7
Or see this region's nucleotide sequence