Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS17640 and RR42_RS17645 are separated by 92 nucleotides RR42_RS17645 and RR42_RS17650 are separated by 17 nucleotides RR42_RS17650 and RR42_RS17655 are separated by 114 nucleotides
RR42_RS17640: RR42_RS17640 - peptidoglycan transglycosylase, at 3,817,125 to 3,817,826
_RS17640
RR42_RS17645: RR42_RS17645 - shikimate dehydrogenase, at 3,817,919 to 3,818,794
_RS17645
RR42_RS17650: RR42_RS17650 - energy transducer TonB, at 3,818,812 to 3,819,729
_RS17650
RR42_RS17655: RR42_RS17655 - ribonuclease II, at 3,819,844 to 3,822,000
_RS17655
Position (kb)
3818
3819
3820 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3818.001 kb on + strand at 3818.001 kb on + strand at 3818.001 kb on + strand at 3818.001 kb on + strand at 3818.002 kb on - strand at 3818.084 kb on + strand, within RR42_RS17645 at 3818.084 kb on + strand, within RR42_RS17645 at 3818.085 kb on - strand, within RR42_RS17645 at 3818.085 kb on - strand, within RR42_RS17645 at 3818.103 kb on + strand, within RR42_RS17645 at 3818.103 kb on + strand, within RR42_RS17645 at 3818.104 kb on - strand, within RR42_RS17645 at 3818.115 kb on + strand, within RR42_RS17645 at 3818.131 kb on + strand, within RR42_RS17645 at 3818.269 kb on - strand, within RR42_RS17645 at 3818.435 kb on - strand, within RR42_RS17645 at 3818.571 kb on + strand, within RR42_RS17645 at 3818.572 kb on - strand, within RR42_RS17645 at 3818.766 kb on + strand at 3818.794 kb on + strand at 3818.794 kb on + strand at 3818.794 kb on + strand at 3818.794 kb on + strand at 3818.794 kb on + strand at 3818.795 kb on - strand at 3818.795 kb on - strand at 3818.795 kb on - strand at 3818.795 kb on - strand at 3818.908 kb on - strand, within RR42_RS17650 at 3819.008 kb on + strand, within RR42_RS17650 at 3819.022 kb on - strand, within RR42_RS17650 at 3819.146 kb on + strand, within RR42_RS17650 at 3819.147 kb on - strand, within RR42_RS17650 at 3819.147 kb on - strand, within RR42_RS17650 at 3819.150 kb on - strand, within RR42_RS17650 at 3819.158 kb on + strand, within RR42_RS17650 at 3819.215 kb on + strand, within RR42_RS17650 at 3819.216 kb on - strand, within RR42_RS17650 at 3819.814 kb on + strand at 3819.815 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,818,001 + -0.0 3,818,001 + +0.5 3,818,001 + -0.1 3,818,001 + -0.5 3,818,002 - -2.2 3,818,084 + RR42_RS17645 0.19 +0.1 3,818,084 + RR42_RS17645 0.19 +0.6 3,818,085 - RR42_RS17645 0.19 -0.3 3,818,085 - RR42_RS17645 0.19 -0.0 3,818,103 + RR42_RS17645 0.21 -0.4 3,818,103 + RR42_RS17645 0.21 +0.3 3,818,104 - RR42_RS17645 0.21 -0.4 3,818,115 + RR42_RS17645 0.22 -0.6 3,818,131 + RR42_RS17645 0.24 -0.9 3,818,269 - RR42_RS17645 0.40 -1.6 3,818,435 - RR42_RS17645 0.59 +1.0 3,818,571 + RR42_RS17645 0.74 -0.6 3,818,572 - RR42_RS17645 0.75 -0.4 3,818,766 + -0.3 3,818,794 + +3.6 3,818,794 + +0.7 3,818,794 + -0.5 3,818,794 + +0.4 3,818,794 + +2.6 3,818,795 - +0.4 3,818,795 - -0.1 3,818,795 - -0.2 3,818,795 - +0.4 3,818,908 - RR42_RS17650 0.10 -0.4 3,819,008 + RR42_RS17650 0.21 -0.1 3,819,022 - RR42_RS17650 0.23 -0.3 3,819,146 + RR42_RS17650 0.36 -0.8 3,819,147 - RR42_RS17650 0.36 -0.2 3,819,147 - RR42_RS17650 0.36 -1.4 3,819,150 - RR42_RS17650 0.37 -0.9 3,819,158 + RR42_RS17650 0.38 +0.2 3,819,215 + RR42_RS17650 0.44 -1.5 3,819,216 - RR42_RS17650 0.44 +1.1 3,819,814 + +1.1 3,819,815 - -0.8
Or see this region's nucleotide sequence