Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS16485 and RR42_RS16490 are separated by 472 nucleotides RR42_RS16490 and RR42_RS16495 are separated by 152 nucleotides
RR42_RS16485: RR42_RS16485 - sugar ABC transporter substrate-binding protein, at 3,571,225 to 3,572,460
_RS16485
RR42_RS16490: RR42_RS16490 - transcriptional regulator, at 3,572,933 to 3,573,865
_RS16490
RR42_RS16495: RR42_RS16495 - branched-chain amino acid aminotransferase, at 3,574,018 to 3,574,839
_RS16495
Position (kb)
3572
3573
3574 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3572.054 kb on + strand, within RR42_RS16485 at 3572.055 kb on - strand, within RR42_RS16485 at 3572.057 kb on + strand, within RR42_RS16485 at 3572.057 kb on + strand, within RR42_RS16485 at 3572.207 kb on + strand, within RR42_RS16485 at 3572.208 kb on - strand, within RR42_RS16485 at 3572.467 kb on + strand at 3572.467 kb on + strand at 3572.468 kb on - strand at 3572.656 kb on + strand at 3572.657 kb on - strand at 3572.657 kb on - strand at 3572.657 kb on - strand at 3572.657 kb on - strand at 3572.660 kb on + strand at 3572.660 kb on + strand at 3572.661 kb on - strand at 3572.700 kb on - strand at 3572.717 kb on + strand at 3572.766 kb on + strand at 3572.766 kb on + strand at 3572.794 kb on + strand at 3572.795 kb on - strand at 3573.039 kb on + strand, within RR42_RS16490 at 3573.040 kb on - strand, within RR42_RS16490 at 3573.040 kb on - strand, within RR42_RS16490 at 3573.040 kb on - strand, within RR42_RS16490 at 3573.045 kb on + strand, within RR42_RS16490 at 3573.045 kb on + strand, within RR42_RS16490 at 3573.046 kb on - strand, within RR42_RS16490 at 3573.285 kb on + strand, within RR42_RS16490 at 3573.286 kb on - strand, within RR42_RS16490 at 3573.286 kb on - strand, within RR42_RS16490 at 3573.286 kb on - strand, within RR42_RS16490 at 3573.286 kb on - strand, within RR42_RS16490 at 3573.409 kb on - strand, within RR42_RS16490 at 3573.479 kb on - strand, within RR42_RS16490 at 3573.479 kb on - strand, within RR42_RS16490 at 3573.751 kb on - strand, within RR42_RS16490 at 3573.903 kb on - strand at 3573.903 kb on - strand at 3573.927 kb on + strand at 3573.928 kb on - strand at 3573.934 kb on + strand at 3573.934 kb on + strand at 3574.041 kb on + strand at 3574.234 kb on + strand, within RR42_RS16495 at 3574.292 kb on - strand, within RR42_RS16495 at 3574.460 kb on - strand, within RR42_RS16495 at 3574.528 kb on - strand, within RR42_RS16495 at 3574.811 kb on - strand at 3574.811 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,572,054 + RR42_RS16485 0.67 -1.7 3,572,055 - RR42_RS16485 0.67 +0.1 3,572,057 + RR42_RS16485 0.67 -1.1 3,572,057 + RR42_RS16485 0.67 +0.2 3,572,207 + RR42_RS16485 0.79 -0.1 3,572,208 - RR42_RS16485 0.80 -0.4 3,572,467 + +0.7 3,572,467 + +0.2 3,572,468 - +0.1 3,572,656 + -0.7 3,572,657 - -0.4 3,572,657 - -1.9 3,572,657 - +0.5 3,572,657 - +1.6 3,572,660 + -1.5 3,572,660 + -0.1 3,572,661 - -0.9 3,572,700 - +0.3 3,572,717 + +0.3 3,572,766 + +0.1 3,572,766 + +0.9 3,572,794 + +0.1 3,572,795 - +1.6 3,573,039 + RR42_RS16490 0.11 +1.0 3,573,040 - RR42_RS16490 0.11 -0.0 3,573,040 - RR42_RS16490 0.11 +0.9 3,573,040 - RR42_RS16490 0.11 +1.0 3,573,045 + RR42_RS16490 0.12 +0.8 3,573,045 + RR42_RS16490 0.12 +1.2 3,573,046 - RR42_RS16490 0.12 -0.5 3,573,285 + RR42_RS16490 0.38 -0.0 3,573,286 - RR42_RS16490 0.38 -1.5 3,573,286 - RR42_RS16490 0.38 -1.0 3,573,286 - RR42_RS16490 0.38 +0.4 3,573,286 - RR42_RS16490 0.38 +0.3 3,573,409 - RR42_RS16490 0.51 -1.4 3,573,479 - RR42_RS16490 0.59 -0.0 3,573,479 - RR42_RS16490 0.59 -0.6 3,573,751 - RR42_RS16490 0.88 +1.4 3,573,903 - -0.3 3,573,903 - -3.2 3,573,927 + -1.8 3,573,928 - -1.0 3,573,934 + +0.4 3,573,934 + +0.8 3,574,041 + +0.3 3,574,234 + RR42_RS16495 0.26 +0.7 3,574,292 - RR42_RS16495 0.33 +1.4 3,574,460 - RR42_RS16495 0.54 -0.9 3,574,528 - RR42_RS16495 0.62 +0.1 3,574,811 - +0.2 3,574,811 - -0.2
Or see this region's nucleotide sequence