Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS16215 and RR42_RS16220 overlap by 4 nucleotides RR42_RS16220 and RR42_RS16225 are separated by 105 nucleotides RR42_RS16225 and RR42_RS16230 are separated by 138 nucleotides
RR42_RS16215: RR42_RS16215 - hypothetical protein, at 3,510,862 to 3,511,455
_RS16215
RR42_RS16220: RR42_RS16220 - DNA polymerase III subunit chi, at 3,511,452 to 3,511,922
_RS16220
RR42_RS16225: RR42_RS16225 - cytosol aminopeptidase, at 3,512,028 to 3,513,596
_RS16225
RR42_RS16230: RR42_RS16230 - permease, at 3,513,735 to 3,514,859
_RS16230
Position (kb) 3512
3513
3514 Strain fitness (log2 ratio) -1
0
1
2
3 at 3511.137 kb on - strand, within RR42_RS16215 at 3511.849 kb on + strand, within RR42_RS16220 at 3511.953 kb on - strand at 3512.143 kb on + strand at 3512.143 kb on + strand at 3512.143 kb on + strand at 3512.143 kb on + strand at 3512.155 kb on + strand at 3512.248 kb on + strand, within RR42_RS16225 at 3512.249 kb on - strand, within RR42_RS16225 at 3512.431 kb on + strand, within RR42_RS16225 at 3512.431 kb on + strand, within RR42_RS16225 at 3512.432 kb on - strand, within RR42_RS16225 at 3512.432 kb on - strand, within RR42_RS16225 at 3512.432 kb on - strand, within RR42_RS16225 at 3512.432 kb on - strand, within RR42_RS16225 at 3512.650 kb on + strand, within RR42_RS16225 at 3512.650 kb on + strand, within RR42_RS16225 at 3512.651 kb on - strand, within RR42_RS16225 at 3512.651 kb on - strand, within RR42_RS16225 at 3512.762 kb on - strand, within RR42_RS16225 at 3512.912 kb on - strand, within RR42_RS16225 at 3512.927 kb on + strand, within RR42_RS16225 at 3512.927 kb on + strand, within RR42_RS16225 at 3512.927 kb on + strand, within RR42_RS16225 at 3512.927 kb on + strand, within RR42_RS16225 at 3512.927 kb on + strand, within RR42_RS16225 at 3513.035 kb on + strand, within RR42_RS16225 at 3513.035 kb on + strand, within RR42_RS16225 at 3513.176 kb on + strand, within RR42_RS16225 at 3513.587 kb on + strand at 3513.636 kb on + strand at 3513.636 kb on + strand at 3513.667 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,511,137 - RR42_RS16215 0.46 -1.0 3,511,849 + RR42_RS16220 0.84 -0.2 3,511,953 - -0.7 3,512,143 + +0.3 3,512,143 + +0.4 3,512,143 + -0.9 3,512,143 + +2.6 3,512,155 + +1.0 3,512,248 + RR42_RS16225 0.14 +1.4 3,512,249 - RR42_RS16225 0.14 +1.0 3,512,431 + RR42_RS16225 0.26 +2.0 3,512,431 + RR42_RS16225 0.26 +2.2 3,512,432 - RR42_RS16225 0.26 +1.9 3,512,432 - RR42_RS16225 0.26 +1.8 3,512,432 - RR42_RS16225 0.26 +2.7 3,512,432 - RR42_RS16225 0.26 +1.2 3,512,650 + RR42_RS16225 0.40 -0.1 3,512,650 + RR42_RS16225 0.40 +1.9 3,512,651 - RR42_RS16225 0.40 +0.1 3,512,651 - RR42_RS16225 0.40 +1.8 3,512,762 - RR42_RS16225 0.47 +1.1 3,512,912 - RR42_RS16225 0.56 +3.0 3,512,927 + RR42_RS16225 0.57 +0.7 3,512,927 + RR42_RS16225 0.57 +1.0 3,512,927 + RR42_RS16225 0.57 +0.6 3,512,927 + RR42_RS16225 0.57 +1.4 3,512,927 + RR42_RS16225 0.57 +0.2 3,513,035 + RR42_RS16225 0.64 +1.7 3,513,035 + RR42_RS16225 0.64 +0.6 3,513,176 + RR42_RS16225 0.73 +1.3 3,513,587 + +0.7 3,513,636 + +1.6 3,513,636 + -0.1 3,513,667 + +1.4
Or see this region's nucleotide sequence