Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS16155 and RR42_RS16160 are separated by 84 nucleotides RR42_RS16160 and RR42_RS16165 are separated by 120 nucleotides RR42_RS16165 and RR42_RS16170 are separated by 129 nucleotides
RR42_RS16155: RR42_RS16155 - RNA-binding protein, at 3,497,875 to 3,498,369
_RS16155
RR42_RS16160: RR42_RS16160 - membrane protein, at 3,498,454 to 3,499,266
_RS16160
RR42_RS16165: RR42_RS16165 - periplasmic or secreted protein, at 3,499,387 to 3,500,088
_RS16165
RR42_RS16170: RR42_RS16170 - malonyl-CoA synthase, at 3,500,218 to 3,501,771
_RS16170
Position (kb)
3498
3499
3500 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3497.546 kb on - strand at 3497.578 kb on + strand at 3497.578 kb on + strand at 3497.578 kb on + strand at 3497.578 kb on + strand at 3497.578 kb on + strand at 3497.578 kb on + strand at 3497.579 kb on - strand at 3497.611 kb on + strand at 3497.695 kb on - strand at 3497.837 kb on + strand at 3497.837 kb on + strand at 3497.837 kb on + strand at 3497.838 kb on - strand at 3498.007 kb on + strand, within RR42_RS16155 at 3498.007 kb on + strand, within RR42_RS16155 at 3498.008 kb on - strand, within RR42_RS16155 at 3498.008 kb on - strand, within RR42_RS16155 at 3498.064 kb on + strand, within RR42_RS16155 at 3498.064 kb on + strand, within RR42_RS16155 at 3498.064 kb on + strand, within RR42_RS16155 at 3498.064 kb on + strand, within RR42_RS16155 at 3498.065 kb on - strand, within RR42_RS16155 at 3498.160 kb on + strand, within RR42_RS16155 at 3498.163 kb on + strand, within RR42_RS16155 at 3498.163 kb on + strand, within RR42_RS16155 at 3498.163 kb on + strand, within RR42_RS16155 at 3498.164 kb on - strand, within RR42_RS16155 at 3498.367 kb on + strand at 3498.367 kb on + strand at 3498.367 kb on + strand at 3498.421 kb on + strand at 3498.421 kb on + strand at 3498.422 kb on - strand at 3498.425 kb on + strand at 3498.426 kb on + strand at 3498.426 kb on - strand at 3498.498 kb on + strand at 3498.606 kb on + strand, within RR42_RS16160 at 3498.633 kb on + strand, within RR42_RS16160 at 3498.800 kb on + strand, within RR42_RS16160 at 3498.800 kb on + strand, within RR42_RS16160 at 3499.045 kb on + strand, within RR42_RS16160 at 3499.046 kb on - strand, within RR42_RS16160 at 3499.046 kb on - strand, within RR42_RS16160 at 3499.046 kb on - strand, within RR42_RS16160 at 3499.096 kb on + strand, within RR42_RS16160 at 3499.102 kb on + strand, within RR42_RS16160 at 3499.102 kb on + strand, within RR42_RS16160 at 3499.103 kb on - strand, within RR42_RS16160 at 3499.103 kb on - strand, within RR42_RS16160 at 3499.103 kb on - strand, within RR42_RS16160 at 3499.103 kb on - strand, within RR42_RS16160 at 3499.103 kb on - strand, within RR42_RS16160 at 3499.103 kb on - strand, within RR42_RS16160 at 3499.234 kb on + strand at 3499.234 kb on + strand at 3499.234 kb on + strand at 3499.235 kb on - strand at 3499.304 kb on + strand at 3499.304 kb on + strand at 3499.304 kb on + strand at 3499.305 kb on - strand at 3499.305 kb on - strand at 3499.308 kb on + strand at 3499.308 kb on + strand at 3499.308 kb on + strand at 3499.309 kb on - strand at 3499.309 kb on - strand at 3499.309 kb on - strand at 3499.420 kb on - strand at 3499.633 kb on - strand, within RR42_RS16165 at 3499.633 kb on - strand, within RR42_RS16165 at 3499.633 kb on - strand, within RR42_RS16165 at 3499.672 kb on + strand, within RR42_RS16165 at 3499.673 kb on - strand, within RR42_RS16165 at 3499.925 kb on - strand, within RR42_RS16165 at 3499.925 kb on - strand, within RR42_RS16165 at 3499.925 kb on - strand, within RR42_RS16165 at 3499.925 kb on - strand, within RR42_RS16165 at 3499.925 kb on - strand, within RR42_RS16165 at 3499.984 kb on + strand, within RR42_RS16165 at 3499.984 kb on + strand, within RR42_RS16165 at 3499.984 kb on + strand, within RR42_RS16165 at 3499.985 kb on - strand, within RR42_RS16165 at 3499.985 kb on - strand, within RR42_RS16165 at 3499.985 kb on - strand, within RR42_RS16165 at 3499.985 kb on - strand, within RR42_RS16165 at 3499.985 kb on - strand, within RR42_RS16165 at 3499.985 kb on - strand, within RR42_RS16165 at 3499.985 kb on - strand, within RR42_RS16165 at 3499.985 kb on - strand, within RR42_RS16165 at 3499.985 kb on - strand, within RR42_RS16165 at 3500.087 kb on - strand at 3500.219 kb on + strand at 3500.220 kb on - strand at 3500.220 kb on - strand at 3500.220 kb on - strand at 3500.220 kb on - strand at 3500.220 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,497,546 - +0.4 3,497,578 + +0.3 3,497,578 + +0.1 3,497,578 + -1.1 3,497,578 + +0.4 3,497,578 + +0.9 3,497,578 + +0.2 3,497,579 - -0.2 3,497,611 + +0.3 3,497,695 - +0.8 3,497,837 + +0.3 3,497,837 + +0.0 3,497,837 + -1.5 3,497,838 - -0.1 3,498,007 + RR42_RS16155 0.27 +0.2 3,498,007 + RR42_RS16155 0.27 -1.3 3,498,008 - RR42_RS16155 0.27 +0.1 3,498,008 - RR42_RS16155 0.27 -0.2 3,498,064 + RR42_RS16155 0.38 -0.5 3,498,064 + RR42_RS16155 0.38 +0.4 3,498,064 + RR42_RS16155 0.38 +1.4 3,498,064 + RR42_RS16155 0.38 +0.1 3,498,065 - RR42_RS16155 0.38 +1.0 3,498,160 + RR42_RS16155 0.58 +0.4 3,498,163 + RR42_RS16155 0.58 -1.8 3,498,163 + RR42_RS16155 0.58 -0.2 3,498,163 + RR42_RS16155 0.58 -2.4 3,498,164 - RR42_RS16155 0.58 +1.4 3,498,367 + -0.7 3,498,367 + +0.8 3,498,367 + -0.4 3,498,421 + -0.2 3,498,421 + -0.8 3,498,422 - -1.4 3,498,425 + +3.1 3,498,426 + +1.6 3,498,426 - +1.0 3,498,498 + -0.4 3,498,606 + RR42_RS16160 0.19 +1.1 3,498,633 + RR42_RS16160 0.22 -0.1 3,498,800 + RR42_RS16160 0.43 -1.2 3,498,800 + RR42_RS16160 0.43 -1.8 3,499,045 + RR42_RS16160 0.73 -0.1 3,499,046 - RR42_RS16160 0.73 -0.8 3,499,046 - RR42_RS16160 0.73 -0.4 3,499,046 - RR42_RS16160 0.73 -1.3 3,499,096 + RR42_RS16160 0.79 +1.7 3,499,102 + RR42_RS16160 0.80 -1.2 3,499,102 + RR42_RS16160 0.80 +0.6 3,499,103 - RR42_RS16160 0.80 -0.2 3,499,103 - RR42_RS16160 0.80 -0.3 3,499,103 - RR42_RS16160 0.80 -1.3 3,499,103 - RR42_RS16160 0.80 -0.6 3,499,103 - RR42_RS16160 0.80 +0.1 3,499,103 - RR42_RS16160 0.80 -0.6 3,499,234 + -2.1 3,499,234 + -0.7 3,499,234 + -0.5 3,499,235 - +0.2 3,499,304 + +0.0 3,499,304 + +0.6 3,499,304 + -1.3 3,499,305 - +1.6 3,499,305 - -0.1 3,499,308 + -0.4 3,499,308 + -0.5 3,499,308 + -0.8 3,499,309 - +0.1 3,499,309 - +0.3 3,499,309 - -0.3 3,499,420 - +0.6 3,499,633 - RR42_RS16165 0.35 +0.0 3,499,633 - RR42_RS16165 0.35 -0.8 3,499,633 - RR42_RS16165 0.35 +0.5 3,499,672 + RR42_RS16165 0.41 -0.1 3,499,673 - RR42_RS16165 0.41 -0.5 3,499,925 - RR42_RS16165 0.77 -0.2 3,499,925 - RR42_RS16165 0.77 -0.4 3,499,925 - RR42_RS16165 0.77 +0.2 3,499,925 - RR42_RS16165 0.77 -0.3 3,499,925 - RR42_RS16165 0.77 -1.7 3,499,984 + RR42_RS16165 0.85 -0.4 3,499,984 + RR42_RS16165 0.85 -0.5 3,499,984 + RR42_RS16165 0.85 -0.7 3,499,985 - RR42_RS16165 0.85 +0.0 3,499,985 - RR42_RS16165 0.85 +1.1 3,499,985 - RR42_RS16165 0.85 +1.6 3,499,985 - RR42_RS16165 0.85 -1.4 3,499,985 - RR42_RS16165 0.85 -0.5 3,499,985 - RR42_RS16165 0.85 +1.0 3,499,985 - RR42_RS16165 0.85 +0.8 3,499,985 - RR42_RS16165 0.85 -0.7 3,499,985 - RR42_RS16165 0.85 +0.4 3,500,087 - -1.3 3,500,219 + -0.5 3,500,220 - +0.3 3,500,220 - -0.7 3,500,220 - -0.6 3,500,220 - -0.2 3,500,220 - -0.2
Or see this region's nucleotide sequence