Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS16135 and RR42_RS16140 are separated by 12 nucleotides RR42_RS16140 and RR42_RS16145 are separated by 552 nucleotides RR42_RS16145 and RR42_RS16150 overlap by 4 nucleotides
RR42_RS16135: RR42_RS16135 - ABC transporter permease, at 3,492,999 to 3,494,006
_RS16135
RR42_RS16140: RR42_RS16140 - TonB-dependent receptor, at 3,494,019 to 3,495,959
_RS16140
RR42_RS16145: RR42_RS16145 - hypothetical protein, at 3,496,512 to 3,496,829
_RS16145
RR42_RS16150: RR42_RS16150 - cell division protein ZapA, at 3,496,826 to 3,497,167
_RS16150
Position (kb)
3494
3495
3496 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3493.238 kb on + strand, within RR42_RS16135 at 3493.239 kb on - strand, within RR42_RS16135 at 3493.239 kb on - strand, within RR42_RS16135 at 3493.411 kb on + strand, within RR42_RS16135 at 3493.411 kb on + strand, within RR42_RS16135 at 3493.412 kb on - strand, within RR42_RS16135 at 3493.735 kb on + strand, within RR42_RS16135 at 3493.735 kb on + strand, within RR42_RS16135 at 3493.736 kb on - strand, within RR42_RS16135 at 3493.736 kb on - strand, within RR42_RS16135 at 3494.089 kb on + strand at 3494.090 kb on - strand at 3494.148 kb on - strand at 3494.309 kb on + strand, within RR42_RS16140 at 3494.435 kb on - strand, within RR42_RS16140 at 3494.527 kb on + strand, within RR42_RS16140 at 3494.527 kb on + strand, within RR42_RS16140 at 3494.527 kb on + strand, within RR42_RS16140 at 3494.528 kb on - strand, within RR42_RS16140 at 3494.528 kb on - strand, within RR42_RS16140 at 3494.594 kb on - strand, within RR42_RS16140 at 3494.594 kb on - strand, within RR42_RS16140 at 3494.596 kb on + strand, within RR42_RS16140 at 3494.596 kb on + strand, within RR42_RS16140 at 3494.597 kb on - strand, within RR42_RS16140 at 3494.597 kb on - strand, within RR42_RS16140 at 3494.671 kb on + strand, within RR42_RS16140 at 3494.690 kb on - strand, within RR42_RS16140 at 3494.771 kb on - strand, within RR42_RS16140 at 3494.812 kb on + strand, within RR42_RS16140 at 3494.812 kb on + strand, within RR42_RS16140 at 3494.843 kb on - strand, within RR42_RS16140 at 3494.902 kb on + strand, within RR42_RS16140 at 3495.008 kb on + strand, within RR42_RS16140 at 3495.034 kb on - strand, within RR42_RS16140 at 3495.040 kb on + strand, within RR42_RS16140 at 3495.041 kb on - strand, within RR42_RS16140 at 3495.041 kb on - strand, within RR42_RS16140 at 3495.071 kb on + strand, within RR42_RS16140 at 3495.095 kb on - strand, within RR42_RS16140 at 3495.095 kb on - strand, within RR42_RS16140 at 3495.115 kb on + strand, within RR42_RS16140 at 3495.191 kb on - strand, within RR42_RS16140 at 3495.276 kb on - strand, within RR42_RS16140 at 3495.343 kb on + strand, within RR42_RS16140 at 3495.465 kb on - strand, within RR42_RS16140 at 3495.701 kb on + strand, within RR42_RS16140 at 3495.701 kb on + strand, within RR42_RS16140 at 3495.702 kb on - strand, within RR42_RS16140 at 3495.943 kb on + strand at 3495.943 kb on + strand at 3495.944 kb on - strand at 3496.176 kb on + strand at 3496.275 kb on + strand at 3496.276 kb on - strand at 3496.302 kb on + strand at 3496.367 kb on + strand at 3496.367 kb on + strand at 3496.368 kb on - strand at 3496.495 kb on + strand at 3496.511 kb on + strand at 3496.834 kb on + strand at 3496.834 kb on + strand at 3496.834 kb on + strand at 3496.834 kb on + strand at 3496.835 kb on - strand at 3496.835 kb on - strand at 3496.835 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,493,238 + RR42_RS16135 0.24 +0.1 3,493,239 - RR42_RS16135 0.24 +1.0 3,493,239 - RR42_RS16135 0.24 -0.3 3,493,411 + RR42_RS16135 0.41 +0.6 3,493,411 + RR42_RS16135 0.41 +1.6 3,493,412 - RR42_RS16135 0.41 +1.7 3,493,735 + RR42_RS16135 0.73 -0.2 3,493,735 + RR42_RS16135 0.73 +0.4 3,493,736 - RR42_RS16135 0.73 -1.0 3,493,736 - RR42_RS16135 0.73 +0.0 3,494,089 + +0.9 3,494,090 - -1.8 3,494,148 - +1.3 3,494,309 + RR42_RS16140 0.15 +1.4 3,494,435 - RR42_RS16140 0.21 -0.5 3,494,527 + RR42_RS16140 0.26 -0.1 3,494,527 + RR42_RS16140 0.26 +0.6 3,494,527 + RR42_RS16140 0.26 -0.3 3,494,528 - RR42_RS16140 0.26 -0.3 3,494,528 - RR42_RS16140 0.26 +0.5 3,494,594 - RR42_RS16140 0.30 -2.7 3,494,594 - RR42_RS16140 0.30 +0.1 3,494,596 + RR42_RS16140 0.30 +0.2 3,494,596 + RR42_RS16140 0.30 -0.3 3,494,597 - RR42_RS16140 0.30 +1.1 3,494,597 - RR42_RS16140 0.30 +1.6 3,494,671 + RR42_RS16140 0.34 +3.6 3,494,690 - RR42_RS16140 0.35 -0.3 3,494,771 - RR42_RS16140 0.39 -1.7 3,494,812 + RR42_RS16140 0.41 -0.2 3,494,812 + RR42_RS16140 0.41 +0.0 3,494,843 - RR42_RS16140 0.42 +0.8 3,494,902 + RR42_RS16140 0.45 -0.1 3,495,008 + RR42_RS16140 0.51 -0.4 3,495,034 - RR42_RS16140 0.52 +4.3 3,495,040 + RR42_RS16140 0.53 +1.0 3,495,041 - RR42_RS16140 0.53 -0.5 3,495,041 - RR42_RS16140 0.53 +0.4 3,495,071 + RR42_RS16140 0.54 +2.3 3,495,095 - RR42_RS16140 0.55 -0.7 3,495,095 - RR42_RS16140 0.55 +1.8 3,495,115 + RR42_RS16140 0.56 -0.6 3,495,191 - RR42_RS16140 0.60 -0.5 3,495,276 - RR42_RS16140 0.65 +0.3 3,495,343 + RR42_RS16140 0.68 +1.6 3,495,465 - RR42_RS16140 0.74 -0.1 3,495,701 + RR42_RS16140 0.87 -0.2 3,495,701 + RR42_RS16140 0.87 -0.9 3,495,702 - RR42_RS16140 0.87 +0.9 3,495,943 + +0.3 3,495,943 + -1.7 3,495,944 - -1.0 3,496,176 + -0.9 3,496,275 + -0.6 3,496,276 - +0.3 3,496,302 + -0.7 3,496,367 + +0.9 3,496,367 + +0.6 3,496,368 - -0.4 3,496,495 + +0.9 3,496,511 + -0.4 3,496,834 + -2.0 3,496,834 + +0.4 3,496,834 + -1.5 3,496,834 + +1.6 3,496,835 - +0.6 3,496,835 - +1.0 3,496,835 - -0.6
Or see this region's nucleotide sequence