Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15565 and RR42_RS15570 are separated by 66 nucleotides RR42_RS15570 and RR42_RS15575 overlap by 8 nucleotides RR42_RS15575 and RR42_RS15580 are separated by 162 nucleotides
RR42_RS15565: RR42_RS15565 - 3,4-dihydroxy-2-butanone 4-phosphate synthase, at 3,361,593 to 3,362,705
_RS15565
RR42_RS15570: RR42_RS15570 - 6,7-dimethyl-8-ribityllumazine synthase, at 3,362,772 to 3,363,275
_RS15570
RR42_RS15575: RR42_RS15575 - N utilization substance protein B, at 3,363,268 to 3,363,789
_RS15575
RR42_RS15580: RR42_RS15580 - aminotransferase, at 3,363,952 to 3,365,133
_RS15580
Position (kb) 3362
3363
3364 Strain fitness (log2 ratio) -1
0
1 at 3362.244 kb on + strand, within RR42_RS15565 at 3362.244 kb on + strand, within RR42_RS15565 at 3362.268 kb on + strand, within RR42_RS15565 at 3362.268 kb on + strand, within RR42_RS15565 at 3362.268 kb on + strand, within RR42_RS15565 at 3362.562 kb on + strand, within RR42_RS15565 at 3362.562 kb on + strand, within RR42_RS15565 at 3363.858 kb on + strand at 3363.891 kb on + strand at 3363.892 kb on - strand at 3363.896 kb on - strand at 3363.926 kb on - strand at 3363.926 kb on - strand at 3363.926 kb on - strand at 3364.154 kb on - strand, within RR42_RS15580 at 3364.154 kb on - strand, within RR42_RS15580 at 3364.204 kb on + strand, within RR42_RS15580 at 3364.204 kb on + strand, within RR42_RS15580 at 3364.204 kb on + strand, within RR42_RS15580 at 3364.204 kb on + strand, within RR42_RS15580 at 3364.204 kb on + strand, within RR42_RS15580 at 3364.205 kb on - strand, within RR42_RS15580 at 3364.216 kb on + strand, within RR42_RS15580 at 3364.216 kb on + strand, within RR42_RS15580 at 3364.217 kb on - strand, within RR42_RS15580
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,362,244 + RR42_RS15565 0.58 +0.9 3,362,244 + RR42_RS15565 0.58 -0.1 3,362,268 + RR42_RS15565 0.61 -0.6 3,362,268 + RR42_RS15565 0.61 -0.2 3,362,268 + RR42_RS15565 0.61 +0.7 3,362,562 + RR42_RS15565 0.87 -0.3 3,362,562 + RR42_RS15565 0.87 +0.3 3,363,858 + +0.6 3,363,891 + -0.1 3,363,892 - -0.8 3,363,896 - -0.0 3,363,926 - +0.2 3,363,926 - -0.1 3,363,926 - -0.1 3,364,154 - RR42_RS15580 0.17 -0.4 3,364,154 - RR42_RS15580 0.17 +0.3 3,364,204 + RR42_RS15580 0.21 -0.7 3,364,204 + RR42_RS15580 0.21 +1.0 3,364,204 + RR42_RS15580 0.21 +0.6 3,364,204 + RR42_RS15580 0.21 +0.6 3,364,204 + RR42_RS15580 0.21 +0.2 3,364,205 - RR42_RS15580 0.21 -1.2 3,364,216 + RR42_RS15580 0.22 -1.1 3,364,216 + RR42_RS15580 0.22 +1.3 3,364,217 - RR42_RS15580 0.22 +1.6
Or see this region's nucleotide sequence