Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15555 and RR42_RS15560 are separated by 84 nucleotides RR42_RS15560 and RR42_RS15565 are separated by 258 nucleotides RR42_RS15565 and RR42_RS15570 are separated by 66 nucleotides RR42_RS15570 and RR42_RS15575 overlap by 8 nucleotides
RR42_RS15555: RR42_RS15555 - diaminohydroxyphosphoribosylaminopyrimidine deaminase, at 3,359,502 to 3,360,620
_RS15555
RR42_RS15560: RR42_RS15560 - riboflavin synthase subunit alpha, at 3,360,705 to 3,361,334
_RS15560
RR42_RS15565: RR42_RS15565 - 3,4-dihydroxy-2-butanone 4-phosphate synthase, at 3,361,593 to 3,362,705
_RS15565
RR42_RS15570: RR42_RS15570 - 6,7-dimethyl-8-ribityllumazine synthase, at 3,362,772 to 3,363,275
_RS15570
RR42_RS15575: RR42_RS15575 - N utilization substance protein B, at 3,363,268 to 3,363,789
_RS15575
Position (kb)
3361
3362
3363 Strain fitness (log2 ratio)
-1
0
1
2 at 3360.668 kb on + strand at 3360.668 kb on + strand at 3360.669 kb on - strand at 3361.460 kb on + strand at 3361.465 kb on + strand at 3361.465 kb on + strand at 3361.743 kb on + strand, within RR42_RS15565 at 3362.244 kb on + strand, within RR42_RS15565 at 3362.244 kb on + strand, within RR42_RS15565 at 3362.268 kb on + strand, within RR42_RS15565 at 3362.268 kb on + strand, within RR42_RS15565 at 3362.268 kb on + strand, within RR42_RS15565 at 3362.562 kb on + strand, within RR42_RS15565 at 3362.562 kb on + strand, within RR42_RS15565
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,360,668 + +0.7 3,360,668 + +2.3 3,360,669 - +0.8 3,361,460 + -0.6 3,361,465 + +0.6 3,361,465 + +1.2 3,361,743 + RR42_RS15565 0.13 -0.8 3,362,244 + RR42_RS15565 0.58 +0.9 3,362,244 + RR42_RS15565 0.58 -0.1 3,362,268 + RR42_RS15565 0.61 -0.6 3,362,268 + RR42_RS15565 0.61 -0.2 3,362,268 + RR42_RS15565 0.61 +0.7 3,362,562 + RR42_RS15565 0.87 -0.3 3,362,562 + RR42_RS15565 0.87 +0.3
Or see this region's nucleotide sequence