Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS15450

Experiment: R2A with Vancomycin 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS15440 and RR42_RS15445 are separated by 65 nucleotidesRR42_RS15445 and RR42_RS15450 are separated by 153 nucleotidesRR42_RS15450 and RR42_RS15455 are separated by 18 nucleotidesRR42_RS15455 and RR42_RS15460 are separated by 26 nucleotides RR42_RS15440: RR42_RS15440 - transporter, at 3,336,696 to 3,337,811 _RS15440 RR42_RS15445: RR42_RS15445 - 7-cyano-7-deazaguanine synthase, at 3,337,877 to 3,338,560 _RS15445 RR42_RS15450: RR42_RS15450 - Tol system periplasmic component YbgF, at 3,338,714 to 3,339,472 _RS15450 RR42_RS15455: RR42_RS15455 - membrane protein, at 3,339,491 to 3,339,997 _RS15455 RR42_RS15460: RR42_RS15460 - translocation protein TolB, at 3,340,024 to 3,341,352 _RS15460 Position (kb) 3338 3339 3340Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3337.773 kb on + strandat 3337.774 kb on - strandat 3337.774 kb on - strandat 3337.774 kb on - strandat 3337.797 kb on - strandat 3337.894 kb on - strandat 3337.991 kb on - strand, within RR42_RS15445at 3337.991 kb on - strand, within RR42_RS15445at 3337.991 kb on - strand, within RR42_RS15445at 3337.992 kb on + strand, within RR42_RS15445at 3337.993 kb on - strand, within RR42_RS15445at 3337.993 kb on - strand, within RR42_RS15445at 3337.993 kb on - strand, within RR42_RS15445at 3338.013 kb on + strand, within RR42_RS15445at 3338.151 kb on + strand, within RR42_RS15445at 3338.151 kb on + strand, within RR42_RS15445at 3338.151 kb on + strand, within RR42_RS15445at 3338.152 kb on - strand, within RR42_RS15445at 3338.152 kb on - strand, within RR42_RS15445at 3338.181 kb on + strand, within RR42_RS15445at 3338.264 kb on - strand, within RR42_RS15445at 3338.281 kb on - strand, within RR42_RS15445at 3338.284 kb on + strand, within RR42_RS15445at 3338.634 kb on + strandat 3338.635 kb on - strandat 3338.659 kb on - strandat 3338.659 kb on - strandat 3338.663 kb on + strandat 3338.664 kb on - strandat 3338.664 kb on - strandat 3338.673 kb on + strandat 3338.673 kb on + strandat 3338.674 kb on - strandat 3338.674 kb on - strandat 3338.715 kb on + strandat 3338.715 kb on + strandat 3338.716 kb on - strandat 3338.769 kb on + strandat 3338.769 kb on + strandat 3338.778 kb on - strandat 3338.778 kb on - strandat 3338.880 kb on + strand, within RR42_RS15450at 3338.881 kb on - strand, within RR42_RS15450at 3338.922 kb on + strand, within RR42_RS15450at 3338.976 kb on + strand, within RR42_RS15450at 3338.977 kb on - strand, within RR42_RS15450at 3339.069 kb on + strand, within RR42_RS15450at 3339.070 kb on - strand, within RR42_RS15450at 3339.166 kb on - strand, within RR42_RS15450at 3339.439 kb on + strandat 3339.439 kb on + strandat 3339.439 kb on + strandat 3339.492 kb on + strandat 3339.493 kb on - strandat 3339.493 kb on - strandat 3339.493 kb on - strandat 3339.495 kb on + strandat 3339.495 kb on + strandat 3339.495 kb on + strandat 3339.496 kb on - strandat 3339.496 kb on - strandat 3339.496 kb on - strandat 3339.496 kb on - strandat 3339.663 kb on + strand, within RR42_RS15455at 3339.762 kb on + strand, within RR42_RS15455at 3339.780 kb on + strand, within RR42_RS15455at 3339.781 kb on - strand, within RR42_RS15455at 3339.781 kb on - strand, within RR42_RS15455at 3339.781 kb on - strand, within RR42_RS15455at 3339.781 kb on - strand, within RR42_RS15455at 3339.781 kb on - strand, within RR42_RS15455at 3339.798 kb on + strand, within RR42_RS15455at 3339.799 kb on - strand, within RR42_RS15455at 3339.892 kb on + strand, within RR42_RS15455at 3339.997 kb on + strandat 3340.011 kb on - strandat 3340.026 kb on - strandat 3340.026 kb on - strandat 3340.319 kb on + strand, within RR42_RS15460at 3340.418 kb on + strand, within RR42_RS15460at 3340.418 kb on + strand, within RR42_RS15460at 3340.418 kb on + strand, within RR42_RS15460at 3340.419 kb on + strand, within RR42_RS15460

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml
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3,337,773 + +0.3
3,337,774 - +0.2
3,337,774 - -0.3
3,337,774 - +2.6
3,337,797 - +1.0
3,337,894 - +0.6
3,337,991 - RR42_RS15445 0.17 +0.0
3,337,991 - RR42_RS15445 0.17 +0.5
3,337,991 - RR42_RS15445 0.17 +0.7
3,337,992 + RR42_RS15445 0.17 +0.1
3,337,993 - RR42_RS15445 0.17 -0.1
3,337,993 - RR42_RS15445 0.17 -0.2
3,337,993 - RR42_RS15445 0.17 +1.6
3,338,013 + RR42_RS15445 0.20 -1.6
3,338,151 + RR42_RS15445 0.40 -0.9
3,338,151 + RR42_RS15445 0.40 -0.2
3,338,151 + RR42_RS15445 0.40 -0.9
3,338,152 - RR42_RS15445 0.40 -0.2
3,338,152 - RR42_RS15445 0.40 -1.8
3,338,181 + RR42_RS15445 0.44 +0.7
3,338,264 - RR42_RS15445 0.57 -0.1
3,338,281 - RR42_RS15445 0.59 +0.6
3,338,284 + RR42_RS15445 0.60 -0.4
3,338,634 + -1.9
3,338,635 - -0.1
3,338,659 - -0.4
3,338,659 - +0.5
3,338,663 + +0.1
3,338,664 - +0.8
3,338,664 - +1.0
3,338,673 + +1.0
3,338,673 + +0.5
3,338,674 - -0.0
3,338,674 - +0.1
3,338,715 + +0.4
3,338,715 + -1.4
3,338,716 - +2.6
3,338,769 + -1.8
3,338,769 + -0.5
3,338,778 - -1.6
3,338,778 - -0.9
3,338,880 + RR42_RS15450 0.22 +0.6
3,338,881 - RR42_RS15450 0.22 -0.1
3,338,922 + RR42_RS15450 0.27 -0.6
3,338,976 + RR42_RS15450 0.35 -0.5
3,338,977 - RR42_RS15450 0.35 -0.4
3,339,069 + RR42_RS15450 0.47 +1.0
3,339,070 - RR42_RS15450 0.47 +1.6
3,339,166 - RR42_RS15450 0.60 -0.8
3,339,439 + -0.9
3,339,439 + -0.9
3,339,439 + -1.5
3,339,492 + -2.3
3,339,493 - -2.5
3,339,493 - -0.6
3,339,493 - +1.0
3,339,495 + -2.7
3,339,495 + -1.5
3,339,495 + -2.4
3,339,496 - +0.2
3,339,496 - +0.1
3,339,496 - -1.9
3,339,496 - -1.0
3,339,663 + RR42_RS15455 0.34 +1.6
3,339,762 + RR42_RS15455 0.53 -1.8
3,339,780 + RR42_RS15455 0.57 -2.1
3,339,781 - RR42_RS15455 0.57 -1.7
3,339,781 - RR42_RS15455 0.57 -1.7
3,339,781 - RR42_RS15455 0.57 -0.8
3,339,781 - RR42_RS15455 0.57 -1.2
3,339,781 - RR42_RS15455 0.57 +1.0
3,339,798 + RR42_RS15455 0.61 -3.1
3,339,799 - RR42_RS15455 0.61 -1.8
3,339,892 + RR42_RS15455 0.79 +1.6
3,339,997 + -1.9
3,340,011 - +0.4
3,340,026 - -0.7
3,340,026 - -1.0
3,340,319 + RR42_RS15460 0.22 +1.6
3,340,418 + RR42_RS15460 0.30 -0.2
3,340,418 + RR42_RS15460 0.30 -1.8
3,340,418 + RR42_RS15460 0.30 +0.6
3,340,419 + RR42_RS15460 0.30 +1.0

Or see this region's nucleotide sequence