Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15235 and RR42_RS15240 are separated by 145 nucleotides RR42_RS15240 and RR42_RS15245 are separated by 99 nucleotides RR42_RS15245 and RR42_RS15250 are separated by 321 nucleotides RR42_RS15250 and RR42_RS15255 are separated by 96 nucleotides
RR42_RS15235: RR42_RS15235 - hypothetical protein, at 3,299,384 to 3,299,986
_RS15235
RR42_RS15240: RR42_RS15240 - membrane protein, at 3,300,132 to 3,300,443
_RS15240
RR42_RS15245: RR42_RS15245 - alpha/beta hydrolase, at 3,300,543 to 3,300,941
_RS15245
RR42_RS15250: RR42_RS15250 - hypothetical protein, at 3,301,263 to 3,301,811
_RS15250
RR42_RS15255: RR42_RS15255 - peptidyl-prolyl cis-trans isomerase, at 3,301,908 to 3,302,255
_RS15255
Position (kb)
3300
3301 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3299.922 kb on + strand, within RR42_RS15235 at 3300.062 kb on - strand at 3300.252 kb on + strand, within RR42_RS15240 at 3300.252 kb on + strand, within RR42_RS15240 at 3300.253 kb on - strand, within RR42_RS15240 at 3300.253 kb on - strand, within RR42_RS15240 at 3300.253 kb on - strand, within RR42_RS15240 at 3300.253 kb on - strand, within RR42_RS15240 at 3300.253 kb on - strand, within RR42_RS15240 at 3300.412 kb on - strand at 3300.412 kb on - strand at 3300.412 kb on - strand at 3300.414 kb on + strand at 3300.426 kb on + strand at 3300.427 kb on - strand at 3300.838 kb on + strand, within RR42_RS15245 at 3300.850 kb on - strand, within RR42_RS15245 at 3300.890 kb on + strand, within RR42_RS15245 at 3301.213 kb on + strand at 3301.213 kb on + strand at 3301.214 kb on - strand at 3301.214 kb on - strand at 3301.271 kb on + strand at 3301.271 kb on + strand at 3301.271 kb on + strand at 3301.271 kb on + strand at 3301.284 kb on + strand at 3301.285 kb on - strand at 3301.285 kb on - strand at 3301.285 kb on - strand at 3301.285 kb on - strand at 3301.285 kb on - strand at 3301.285 kb on - strand at 3301.324 kb on - strand, within RR42_RS15250 at 3301.326 kb on + strand, within RR42_RS15250 at 3301.327 kb on - strand, within RR42_RS15250 at 3301.327 kb on - strand, within RR42_RS15250 at 3301.347 kb on - strand, within RR42_RS15250 at 3301.347 kb on - strand, within RR42_RS15250 at 3301.347 kb on - strand, within RR42_RS15250 at 3301.347 kb on - strand, within RR42_RS15250 at 3301.581 kb on + strand, within RR42_RS15250 at 3301.582 kb on - strand, within RR42_RS15250 at 3301.582 kb on - strand, within RR42_RS15250 at 3301.582 kb on - strand, within RR42_RS15250 at 3301.582 kb on - strand, within RR42_RS15250 at 3301.656 kb on + strand, within RR42_RS15250 at 3301.656 kb on + strand, within RR42_RS15250 at 3301.657 kb on - strand, within RR42_RS15250 at 3301.657 kb on - strand, within RR42_RS15250 at 3301.657 kb on - strand, within RR42_RS15250 at 3301.887 kb on + strand at 3301.887 kb on + strand at 3301.913 kb on + strand at 3301.913 kb on + strand at 3301.913 kb on + strand at 3301.914 kb on - strand at 3301.914 kb on - strand at 3301.914 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,299,922 + RR42_RS15235 0.89 -1.9 3,300,062 - -0.3 3,300,252 + RR42_RS15240 0.38 -1.6 3,300,252 + RR42_RS15240 0.38 -0.6 3,300,253 - RR42_RS15240 0.39 +0.2 3,300,253 - RR42_RS15240 0.39 -0.1 3,300,253 - RR42_RS15240 0.39 -0.3 3,300,253 - RR42_RS15240 0.39 -0.6 3,300,253 - RR42_RS15240 0.39 -0.0 3,300,412 - -0.2 3,300,412 - -0.0 3,300,412 - +2.1 3,300,414 + +0.3 3,300,426 + -0.7 3,300,427 - -0.2 3,300,838 + RR42_RS15245 0.74 -1.3 3,300,850 - RR42_RS15245 0.77 -0.8 3,300,890 + RR42_RS15245 0.87 +1.8 3,301,213 + +0.6 3,301,213 + +0.5 3,301,214 - -1.1 3,301,214 - +0.8 3,301,271 + -1.4 3,301,271 + -0.4 3,301,271 + +1.2 3,301,271 + -0.6 3,301,284 + +0.6 3,301,285 - -0.2 3,301,285 - -1.3 3,301,285 - +0.2 3,301,285 - -0.1 3,301,285 - -0.8 3,301,285 - -1.4 3,301,324 - RR42_RS15250 0.11 -0.4 3,301,326 + RR42_RS15250 0.11 -0.0 3,301,327 - RR42_RS15250 0.12 +0.7 3,301,327 - RR42_RS15250 0.12 +0.2 3,301,347 - RR42_RS15250 0.15 -0.2 3,301,347 - RR42_RS15250 0.15 +0.1 3,301,347 - RR42_RS15250 0.15 -0.7 3,301,347 - RR42_RS15250 0.15 -0.4 3,301,581 + RR42_RS15250 0.58 -1.0 3,301,582 - RR42_RS15250 0.58 -0.9 3,301,582 - RR42_RS15250 0.58 -0.9 3,301,582 - RR42_RS15250 0.58 -0.9 3,301,582 - RR42_RS15250 0.58 -0.9 3,301,656 + RR42_RS15250 0.72 +1.0 3,301,656 + RR42_RS15250 0.72 -0.8 3,301,657 - RR42_RS15250 0.72 -0.8 3,301,657 - RR42_RS15250 0.72 +1.8 3,301,657 - RR42_RS15250 0.72 -0.1 3,301,887 + -0.9 3,301,887 + -0.3 3,301,913 + +1.6 3,301,913 + -0.4 3,301,913 + +0.1 3,301,914 - +0.9 3,301,914 - -0.0 3,301,914 - -0.3
Or see this region's nucleotide sequence