Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14350 and RR42_RS14355 are separated by 2 nucleotides RR42_RS14355 and RR42_RS14360 overlap by 1 nucleotides RR42_RS14360 and RR42_RS14365 are separated by 127 nucleotides RR42_RS14365 and RR42_RS14370 overlap by 17 nucleotides
RR42_RS14350: RR42_RS14350 - tryptophan synthase alpha chain, at 3,085,216 to 3,086,013
_RS14350
RR42_RS14355: RR42_RS14355 - DNA methylase N-4, at 3,086,016 to 3,086,924
_RS14355
RR42_RS14360: RR42_RS14360 - tryptophan synthase subunit alpha, at 3,086,924 to 3,088,117
_RS14360
RR42_RS14365: RR42_RS14365 - N-(5'-phosphoribosyl)anthranilate isomerase, at 3,088,245 to 3,088,946
_RS14365
RR42_RS14370: RR42_RS14370 - tRNA pseudouridine synthase A, at 3,088,930 to 3,089,754
_RS14370
Position (kb)
3086
3087
3088
3089 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3086.292 kb on - strand, within RR42_RS14355 at 3086.377 kb on + strand, within RR42_RS14355 at 3086.378 kb on - strand, within RR42_RS14355 at 3086.477 kb on - strand, within RR42_RS14355 at 3086.480 kb on - strand, within RR42_RS14355 at 3086.527 kb on + strand, within RR42_RS14355 at 3086.528 kb on - strand, within RR42_RS14355 at 3086.630 kb on - strand, within RR42_RS14355 at 3086.630 kb on - strand, within RR42_RS14355 at 3086.650 kb on + strand, within RR42_RS14355 at 3086.650 kb on + strand, within RR42_RS14355 at 3086.651 kb on - strand, within RR42_RS14355 at 3086.753 kb on - strand, within RR42_RS14355 at 3086.846 kb on - strand at 3086.957 kb on - strand at 3086.957 kb on - strand at 3086.957 kb on - strand at 3086.962 kb on + strand at 3087.040 kb on - strand at 3087.139 kb on - strand, within RR42_RS14360 at 3087.711 kb on + strand, within RR42_RS14360 at 3088.017 kb on + strand at 3088.077 kb on + strand at 3088.335 kb on + strand, within RR42_RS14365 at 3088.335 kb on + strand, within RR42_RS14365 at 3088.710 kb on - strand at 3088.819 kb on + strand, within RR42_RS14365
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,086,292 - RR42_RS14355 0.30 -1.3 3,086,377 + RR42_RS14355 0.40 +0.5 3,086,378 - RR42_RS14355 0.40 -1.5 3,086,477 - RR42_RS14355 0.51 -0.1 3,086,480 - RR42_RS14355 0.51 -0.5 3,086,527 + RR42_RS14355 0.56 +0.5 3,086,528 - RR42_RS14355 0.56 +0.3 3,086,630 - RR42_RS14355 0.68 -0.0 3,086,630 - RR42_RS14355 0.68 -0.4 3,086,650 + RR42_RS14355 0.70 +2.5 3,086,650 + RR42_RS14355 0.70 +0.2 3,086,651 - RR42_RS14355 0.70 -0.1 3,086,753 - RR42_RS14355 0.81 +0.6 3,086,846 - -0.0 3,086,957 - -0.8 3,086,957 - -0.2 3,086,957 - +0.0 3,086,962 + +0.7 3,087,040 - +0.9 3,087,139 - RR42_RS14360 0.18 -1.9 3,087,711 + RR42_RS14360 0.66 +0.9 3,088,017 + +0.5 3,088,077 + -0.3 3,088,335 + RR42_RS14365 0.13 +1.1 3,088,335 + RR42_RS14365 0.13 -1.2 3,088,710 - +0.5 3,088,819 + RR42_RS14365 0.82 -1.4
Or see this region's nucleotide sequence