Experiment: R2A with Vancomycin 0.5 mg/ml 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt RR42_RS14070 and RR42_RS14075 are separated by 8 nucleotides RR42_RS14075 and RR42_RS14080 are separated by 134 nucleotides RR42_RS14080 and RR42_RS14085 are separated by 129 nucleotides RR42_RS14085 and RR42_RS14090 are separated by 64 nucleotides  
        
        RR42_RS14070: RR42_RS14070 - sugar dehydratase, at 3,028,516 to 3,029,589 
         
        _RS14070 
         
        
        RR42_RS14075: RR42_RS14075 - anti-sigma factor, at 3,029,598 to 3,030,227 
         
        _RS14075 
         
        
        RR42_RS14080: RR42_RS14080 - RNA polymerase sigma factor RpoE, at 3,030,362 to 3,030,961 
         
        _RS14080 
         
        
        RR42_RS14085: RR42_RS14085 - hypothetical protein, at 3,031,091 to 3,031,546 
         
        _RS14085 
         
        
        RR42_RS14090: RR42_RS14090 - 3-oxoacyl-ACP synthase, at 3,031,611 to 3,032,843 
         
        _RS14090 
         Position (kb)  
3030 
 
3031 Strain fitness (log2 ratio)  
-5 
 
-4 
 
-3 
 
-2 
 
-1 
 
0 
 
1 
 
2 
 
3 
 
4 at 3029.382 kb on - strand at 3029.382 kb on - strand, within RR42_RS14070 at 3029.399 kb on + strand, within RR42_RS14070 at 3029.400 kb on - strand, within RR42_RS14070 at 3029.466 kb on + strand, within RR42_RS14070 at 3029.466 kb on + strand, within RR42_RS14070 at 3029.466 kb on + strand, within RR42_RS14070 at 3029.466 kb on + strand, within RR42_RS14070 at 3029.583 kb on + strand at 3029.583 kb on + strand at 3029.599 kb on + strand at 3029.600 kb on - strand at 3029.600 kb on - strand at 3029.701 kb on + strand, within RR42_RS14075 at 3029.701 kb on + strand, within RR42_RS14075 at 3029.701 kb on + strand, within RR42_RS14075 at 3030.079 kb on + strand, within RR42_RS14075 at 3030.079 kb on + strand, within RR42_RS14075 at 3030.079 kb on + strand, within RR42_RS14075 at 3030.079 kb on + strand, within RR42_RS14075 at 3030.079 kb on + strand, within RR42_RS14075 at 3030.079 kb on + strand, within RR42_RS14075 at 3030.080 kb on - strand, within RR42_RS14075 at 3030.080 kb on - strand, within RR42_RS14075 at 3030.080 kb on - strand, within RR42_RS14075 at 3030.135 kb on + strand, within RR42_RS14075 at 3030.135 kb on + strand, within RR42_RS14075 at 3030.135 kb on + strand, within RR42_RS14075 at 3030.136 kb on - strand, within RR42_RS14075 at 3030.212 kb on + strand at 3030.212 kb on + strand at 3030.212 kb on + strand at 3030.212 kb on + strand at 3030.213 kb on - strand at 3030.271 kb on + strand at 3030.329 kb on + strand at 3030.330 kb on - strand at 3030.334 kb on - strand at 3030.340 kb on + strand at 3030.346 kb on + strand at 3030.347 kb on - strand at 3030.351 kb on - strand at 3030.351 kb on - strand at 3030.351 kb on - strand at 3030.356 kb on + strand at 3030.357 kb on - strand at 3030.363 kb on + strand at 3030.490 kb on - strand, within RR42_RS14080 at 3030.587 kb on - strand, within RR42_RS14080 at 3030.607 kb on + strand, within RR42_RS14080 at 3030.608 kb on - strand, within RR42_RS14080 at 3030.613 kb on + strand, within RR42_RS14080 at 3030.614 kb on - strand, within RR42_RS14080 at 3030.614 kb on - strand, within RR42_RS14080 at 3030.621 kb on - strand, within RR42_RS14080 at 3030.621 kb on - strand, within RR42_RS14080 at 3030.705 kb on + strand, within RR42_RS14080 at 3030.705 kb on + strand, within RR42_RS14080 at 3030.705 kb on + strand, within RR42_RS14080 at 3030.705 kb on + strand, within RR42_RS14080 at 3030.706 kb on - strand, within RR42_RS14080 at 3030.706 kb on - strand, within RR42_RS14080 at 3030.747 kb on + strand, within RR42_RS14080 at 3030.786 kb on + strand, within RR42_RS14080 at 3030.787 kb on - strand, within RR42_RS14080 at 3030.787 kb on - strand, within RR42_RS14080 at 3030.876 kb on + strand, within RR42_RS14080 at 3030.877 kb on - strand, within RR42_RS14080 at 3030.877 kb on - strand, within RR42_RS14080 at 3030.966 kb on - strand at 3030.966 kb on - strand at 3030.981 kb on + strand at 3030.982 kb on - strand at 3030.982 kb on - strand at 3030.982 kb on - strand at 3031.014 kb on + strand at 3031.024 kb on - strand at 3031.024 kb on - strand at 3031.024 kb on - strand at 3031.024 kb on - strand at 3031.060 kb on + strand at 3031.061 kb on - strand at 3031.061 kb on - strand at 3031.061 kb on - strand at 3031.081 kb on + strand at 3031.082 kb on - strand at 3031.138 kb on + strand, within RR42_RS14085 at 3031.139 kb on - strand, within RR42_RS14085 at 3031.304 kb on - strand, within RR42_RS14085 at 3031.304 kb on - strand, within RR42_RS14085 at 3031.304 kb on - strand, within RR42_RS14085 at 3031.304 kb on - strand, within RR42_RS14085 at 3031.359 kb on + strand, within RR42_RS14085 at 3031.360 kb on - strand, within RR42_RS14085 at 3031.363 kb on + strand, within RR42_RS14085 at 3031.363 kb on + strand, within RR42_RS14085 at 3031.363 kb on + strand, within RR42_RS14085  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   R2A with Vancomycin 0.5 mg/ml             remove   3,029,382   -        -0.1   3,029,382   -    RR42_RS14070   0.81  +1.3   3,029,399   +    RR42_RS14070   0.82  +2.7   3,029,400   -    RR42_RS14070   0.82  +1.7   3,029,466   +    RR42_RS14070   0.88  +3.7   3,029,466   +    RR42_RS14070   0.88  +2.4   3,029,466   +    RR42_RS14070   0.88  +2.2   3,029,466   +    RR42_RS14070   0.88  +1.9   3,029,583   +        +2.8   3,029,583   +        +2.4   3,029,599   +        -0.5   3,029,600   -        +2.1   3,029,600   -        +0.9   3,029,701   +    RR42_RS14075   0.16  +1.2   3,029,701   +    RR42_RS14075   0.16  +0.6   3,029,701   +    RR42_RS14075   0.16  +1.7   3,030,079   +    RR42_RS14075   0.76  +1.9   3,030,079   +    RR42_RS14075   0.76  +2.5   3,030,079   +    RR42_RS14075   0.76  +3.9   3,030,079   +    RR42_RS14075   0.76  +2.9   3,030,079   +    RR42_RS14075   0.76  +2.6   3,030,079   +    RR42_RS14075   0.76  +2.4   3,030,080   -    RR42_RS14075   0.77  +1.9   3,030,080   -    RR42_RS14075   0.77  +2.5   3,030,080   -    RR42_RS14075   0.77  -0.3   3,030,135   +    RR42_RS14075   0.85  +1.8   3,030,135   +    RR42_RS14075   0.85  +2.0   3,030,135   +    RR42_RS14075   0.85  +2.9   3,030,136   -    RR42_RS14075   0.85  +2.2   3,030,212   +        +2.0   3,030,212   +        +2.5   3,030,212   +        +2.5   3,030,212   +        +2.3   3,030,213   -        +3.8   3,030,271   +        +3.3   3,030,329   +        +2.7   3,030,330   -        -2.1   3,030,334   -        -0.7   3,030,340   +        +0.9   3,030,346   +        +1.9   3,030,347   -        -0.1   3,030,351   -        -0.7   3,030,351   -        +1.1   3,030,351   -        -1.6   3,030,356   +        +2.6   3,030,357   -        +0.0   3,030,363   +        +1.9   3,030,490   -    RR42_RS14080   0.21  -1.8   3,030,587   -    RR42_RS14080   0.38  -2.2   3,030,607   +    RR42_RS14080   0.41  +0.5   3,030,608   -    RR42_RS14080   0.41  -0.7   3,030,613   +    RR42_RS14080   0.42  -1.0   3,030,614   -    RR42_RS14080   0.42  -0.3   3,030,614   -    RR42_RS14080   0.42  -1.3   3,030,621   -    RR42_RS14080   0.43  -3.4   3,030,621   -    RR42_RS14080   0.43  -3.3   3,030,705   +    RR42_RS14080   0.57  -2.4   3,030,705   +    RR42_RS14080   0.57  -1.9   3,030,705   +    RR42_RS14080   0.57  -0.1   3,030,705   +    RR42_RS14080   0.57  -3.0   3,030,706   -    RR42_RS14080   0.57  -1.6   3,030,706   -    RR42_RS14080   0.57  -3.0   3,030,747   +    RR42_RS14080   0.64  -2.3   3,030,786   +    RR42_RS14080   0.71  +1.1   3,030,787   -    RR42_RS14080   0.71  -0.7   3,030,787   -    RR42_RS14080   0.71  -3.2   3,030,876   +    RR42_RS14080   0.86  -1.1   3,030,877   -    RR42_RS14080   0.86  -4.8   3,030,877   -    RR42_RS14080   0.86  -1.2   3,030,966   -        -0.7   3,030,966   -        -2.2   3,030,981   +        -1.0   3,030,982   -        -2.4   3,030,982   -        +0.5   3,030,982   -        -0.3   3,031,014   +        +0.4   3,031,024   -        +0.1   3,031,024   -        -0.4   3,031,024   -        +0.5   3,031,024   -        -0.1   3,031,060   +        -1.3   3,031,061   -        -0.5   3,031,061   -        +0.5   3,031,061   -        +0.4   3,031,081   +        -2.3   3,031,082   -        +0.1   3,031,138   +    RR42_RS14085   0.10  -2.3   3,031,139   -    RR42_RS14085   0.11  -0.9   3,031,304   -    RR42_RS14085   0.47  +0.8   3,031,304   -    RR42_RS14085   0.47  +1.2   3,031,304   -    RR42_RS14085   0.47  -1.1   3,031,304   -    RR42_RS14085   0.47  +0.1   3,031,359   +    RR42_RS14085   0.59  -0.1   3,031,360   -    RR42_RS14085   0.59  +0.5   3,031,363   +    RR42_RS14085   0.60  -0.7   3,031,363   +    RR42_RS14085   0.60  -1.1   3,031,363   +    RR42_RS14085   0.60  -0.7 
 
Or see this region's nucleotide sequence