Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS14075

Experiment: R2A with Vancomycin 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS14070 and RR42_RS14075 are separated by 8 nucleotidesRR42_RS14075 and RR42_RS14080 are separated by 134 nucleotidesRR42_RS14080 and RR42_RS14085 are separated by 129 nucleotides RR42_RS14070: RR42_RS14070 - sugar dehydratase, at 3,028,516 to 3,029,589 _RS14070 RR42_RS14075: RR42_RS14075 - anti-sigma factor, at 3,029,598 to 3,030,227 _RS14075 RR42_RS14080: RR42_RS14080 - RNA polymerase sigma factor RpoE, at 3,030,362 to 3,030,961 _RS14080 RR42_RS14085: RR42_RS14085 - hypothetical protein, at 3,031,091 to 3,031,546 _RS14085 Position (kb) 3029 3030 3031Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3 4at 3028.621 kb on + strandat 3028.693 kb on - strand, within RR42_RS14070at 3028.724 kb on + strand, within RR42_RS14070at 3028.864 kb on - strand, within RR42_RS14070at 3028.889 kb on + strand, within RR42_RS14070at 3028.889 kb on + strand, within RR42_RS14070at 3028.889 kb on + strand, within RR42_RS14070at 3028.890 kb on - strand, within RR42_RS14070at 3028.890 kb on - strand, within RR42_RS14070at 3028.890 kb on - strand, within RR42_RS14070at 3029.143 kb on - strand, within RR42_RS14070at 3029.147 kb on + strand, within RR42_RS14070at 3029.147 kb on + strand, within RR42_RS14070at 3029.147 kb on + strand, within RR42_RS14070at 3029.147 kb on + strand, within RR42_RS14070at 3029.147 kb on + strand, within RR42_RS14070at 3029.147 kb on + strand, within RR42_RS14070at 3029.147 kb on + strand, within RR42_RS14070at 3029.148 kb on - strand, within RR42_RS14070at 3029.148 kb on - strand, within RR42_RS14070at 3029.148 kb on - strand, within RR42_RS14070at 3029.148 kb on - strand, within RR42_RS14070at 3029.148 kb on - strand, within RR42_RS14070at 3029.148 kb on - strand, within RR42_RS14070at 3029.315 kb on + strand, within RR42_RS14070at 3029.315 kb on + strand, within RR42_RS14070at 3029.316 kb on - strand, within RR42_RS14070at 3029.339 kb on + strand, within RR42_RS14070at 3029.339 kb on + strand, within RR42_RS14070at 3029.340 kb on - strand, within RR42_RS14070at 3029.350 kb on - strand, within RR42_RS14070at 3029.382 kb on - strandat 3029.382 kb on - strand, within RR42_RS14070at 3029.399 kb on + strand, within RR42_RS14070at 3029.400 kb on - strand, within RR42_RS14070at 3029.466 kb on + strand, within RR42_RS14070at 3029.466 kb on + strand, within RR42_RS14070at 3029.466 kb on + strand, within RR42_RS14070at 3029.466 kb on + strand, within RR42_RS14070at 3029.583 kb on + strandat 3029.583 kb on + strandat 3029.599 kb on + strandat 3029.600 kb on - strandat 3029.600 kb on - strandat 3029.701 kb on + strand, within RR42_RS14075at 3029.701 kb on + strand, within RR42_RS14075at 3029.701 kb on + strand, within RR42_RS14075at 3030.079 kb on + strand, within RR42_RS14075at 3030.079 kb on + strand, within RR42_RS14075at 3030.079 kb on + strand, within RR42_RS14075at 3030.079 kb on + strand, within RR42_RS14075at 3030.079 kb on + strand, within RR42_RS14075at 3030.079 kb on + strand, within RR42_RS14075at 3030.080 kb on - strand, within RR42_RS14075at 3030.080 kb on - strand, within RR42_RS14075at 3030.080 kb on - strand, within RR42_RS14075at 3030.135 kb on + strand, within RR42_RS14075at 3030.135 kb on + strand, within RR42_RS14075at 3030.135 kb on + strand, within RR42_RS14075at 3030.136 kb on - strand, within RR42_RS14075at 3030.212 kb on + strandat 3030.212 kb on + strandat 3030.212 kb on + strandat 3030.212 kb on + strandat 3030.213 kb on - strandat 3030.271 kb on + strandat 3030.329 kb on + strandat 3030.330 kb on - strandat 3030.334 kb on - strandat 3030.340 kb on + strandat 3030.346 kb on + strandat 3030.347 kb on - strandat 3030.351 kb on - strandat 3030.351 kb on - strandat 3030.351 kb on - strandat 3030.356 kb on + strandat 3030.357 kb on - strandat 3030.363 kb on + strandat 3030.490 kb on - strand, within RR42_RS14080at 3030.587 kb on - strand, within RR42_RS14080at 3030.607 kb on + strand, within RR42_RS14080at 3030.608 kb on - strand, within RR42_RS14080at 3030.613 kb on + strand, within RR42_RS14080at 3030.614 kb on - strand, within RR42_RS14080at 3030.614 kb on - strand, within RR42_RS14080at 3030.621 kb on - strand, within RR42_RS14080at 3030.621 kb on - strand, within RR42_RS14080at 3030.705 kb on + strand, within RR42_RS14080at 3030.705 kb on + strand, within RR42_RS14080at 3030.705 kb on + strand, within RR42_RS14080at 3030.705 kb on + strand, within RR42_RS14080at 3030.706 kb on - strand, within RR42_RS14080at 3030.706 kb on - strand, within RR42_RS14080at 3030.747 kb on + strand, within RR42_RS14080at 3030.786 kb on + strand, within RR42_RS14080at 3030.787 kb on - strand, within RR42_RS14080at 3030.787 kb on - strand, within RR42_RS14080at 3030.876 kb on + strand, within RR42_RS14080at 3030.877 kb on - strand, within RR42_RS14080at 3030.877 kb on - strand, within RR42_RS14080at 3030.966 kb on - strandat 3030.966 kb on - strandat 3030.981 kb on + strandat 3030.982 kb on - strandat 3030.982 kb on - strandat 3030.982 kb on - strandat 3031.014 kb on + strandat 3031.024 kb on - strandat 3031.024 kb on - strandat 3031.024 kb on - strandat 3031.024 kb on - strandat 3031.060 kb on + strandat 3031.061 kb on - strandat 3031.061 kb on - strandat 3031.061 kb on - strandat 3031.081 kb on + strandat 3031.082 kb on - strandat 3031.138 kb on + strand, within RR42_RS14085at 3031.139 kb on - strand, within RR42_RS14085

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml
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3,028,621 + +2.4
3,028,693 - RR42_RS14070 0.16 +2.7
3,028,724 + RR42_RS14070 0.19 +0.9
3,028,864 - RR42_RS14070 0.32 +0.7
3,028,889 + RR42_RS14070 0.35 +2.7
3,028,889 + RR42_RS14070 0.35 +0.3
3,028,889 + RR42_RS14070 0.35 +2.1
3,028,890 - RR42_RS14070 0.35 +1.9
3,028,890 - RR42_RS14070 0.35 +3.5
3,028,890 - RR42_RS14070 0.35 +1.9
3,029,143 - RR42_RS14070 0.58 +1.3
3,029,147 + RR42_RS14070 0.59 +2.8
3,029,147 + RR42_RS14070 0.59 +1.8
3,029,147 + RR42_RS14070 0.59 +2.3
3,029,147 + RR42_RS14070 0.59 +2.3
3,029,147 + RR42_RS14070 0.59 +2.1
3,029,147 + RR42_RS14070 0.59 +1.5
3,029,147 + RR42_RS14070 0.59 +1.6
3,029,148 - RR42_RS14070 0.59 +1.8
3,029,148 - RR42_RS14070 0.59 +3.0
3,029,148 - RR42_RS14070 0.59 +2.2
3,029,148 - RR42_RS14070 0.59 +2.4
3,029,148 - RR42_RS14070 0.59 +1.7
3,029,148 - RR42_RS14070 0.59 +1.5
3,029,315 + RR42_RS14070 0.74 +2.3
3,029,315 + RR42_RS14070 0.74 +2.2
3,029,316 - RR42_RS14070 0.74 +2.1
3,029,339 + RR42_RS14070 0.77 +2.9
3,029,339 + RR42_RS14070 0.77 +2.0
3,029,340 - RR42_RS14070 0.77 +2.0
3,029,350 - RR42_RS14070 0.78 +0.6
3,029,382 - -0.1
3,029,382 - RR42_RS14070 0.81 +1.3
3,029,399 + RR42_RS14070 0.82 +2.7
3,029,400 - RR42_RS14070 0.82 +1.7
3,029,466 + RR42_RS14070 0.88 +3.7
3,029,466 + RR42_RS14070 0.88 +2.4
3,029,466 + RR42_RS14070 0.88 +2.2
3,029,466 + RR42_RS14070 0.88 +1.9
3,029,583 + +2.8
3,029,583 + +2.4
3,029,599 + -0.5
3,029,600 - +2.1
3,029,600 - +0.9
3,029,701 + RR42_RS14075 0.16 +1.2
3,029,701 + RR42_RS14075 0.16 +0.6
3,029,701 + RR42_RS14075 0.16 +1.7
3,030,079 + RR42_RS14075 0.76 +1.9
3,030,079 + RR42_RS14075 0.76 +2.5
3,030,079 + RR42_RS14075 0.76 +3.9
3,030,079 + RR42_RS14075 0.76 +2.9
3,030,079 + RR42_RS14075 0.76 +2.6
3,030,079 + RR42_RS14075 0.76 +2.4
3,030,080 - RR42_RS14075 0.77 +1.9
3,030,080 - RR42_RS14075 0.77 +2.5
3,030,080 - RR42_RS14075 0.77 -0.3
3,030,135 + RR42_RS14075 0.85 +1.8
3,030,135 + RR42_RS14075 0.85 +2.0
3,030,135 + RR42_RS14075 0.85 +2.9
3,030,136 - RR42_RS14075 0.85 +2.2
3,030,212 + +2.0
3,030,212 + +2.5
3,030,212 + +2.5
3,030,212 + +2.3
3,030,213 - +3.8
3,030,271 + +3.3
3,030,329 + +2.7
3,030,330 - -2.1
3,030,334 - -0.7
3,030,340 + +0.9
3,030,346 + +1.9
3,030,347 - -0.1
3,030,351 - -0.7
3,030,351 - +1.1
3,030,351 - -1.6
3,030,356 + +2.6
3,030,357 - +0.0
3,030,363 + +1.9
3,030,490 - RR42_RS14080 0.21 -1.8
3,030,587 - RR42_RS14080 0.38 -2.2
3,030,607 + RR42_RS14080 0.41 +0.5
3,030,608 - RR42_RS14080 0.41 -0.7
3,030,613 + RR42_RS14080 0.42 -1.0
3,030,614 - RR42_RS14080 0.42 -0.3
3,030,614 - RR42_RS14080 0.42 -1.3
3,030,621 - RR42_RS14080 0.43 -3.4
3,030,621 - RR42_RS14080 0.43 -3.3
3,030,705 + RR42_RS14080 0.57 -2.4
3,030,705 + RR42_RS14080 0.57 -1.9
3,030,705 + RR42_RS14080 0.57 -0.1
3,030,705 + RR42_RS14080 0.57 -3.0
3,030,706 - RR42_RS14080 0.57 -1.6
3,030,706 - RR42_RS14080 0.57 -3.0
3,030,747 + RR42_RS14080 0.64 -2.3
3,030,786 + RR42_RS14080 0.71 +1.1
3,030,787 - RR42_RS14080 0.71 -0.7
3,030,787 - RR42_RS14080 0.71 -3.2
3,030,876 + RR42_RS14080 0.86 -1.1
3,030,877 - RR42_RS14080 0.86 -4.8
3,030,877 - RR42_RS14080 0.86 -1.2
3,030,966 - -0.7
3,030,966 - -2.2
3,030,981 + -1.0
3,030,982 - -2.4
3,030,982 - +0.5
3,030,982 - -0.3
3,031,014 + +0.4
3,031,024 - +0.1
3,031,024 - -0.4
3,031,024 - +0.5
3,031,024 - -0.1
3,031,060 + -1.3
3,031,061 - -0.5
3,031,061 - +0.5
3,031,061 - +0.4
3,031,081 + -2.3
3,031,082 - +0.1
3,031,138 + RR42_RS14085 0.10 -2.3
3,031,139 - RR42_RS14085 0.11 -0.9

Or see this region's nucleotide sequence