Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS13355 and RR42_RS13360 are separated by 248 nucleotides RR42_RS13360 and RR42_RS13365 are separated by 80 nucleotides
RR42_RS13355: RR42_RS13355 - glutamyl-tRNA synthetase, at 2,879,687 to 2,881,093
_RS13355
RR42_RS13360: RR42_RS13360 - esterase, at 2,881,342 to 2,882,292
_RS13360
RR42_RS13365: RR42_RS13365 - peptidase P60, at 2,882,373 to 2,883,059
_RS13365
Position (kb)
2881
2882
2883 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2881.145 kb on - strand at 2881.159 kb on - strand at 2881.160 kb on + strand at 2881.160 kb on + strand at 2881.160 kb on + strand at 2881.161 kb on - strand at 2881.161 kb on - strand at 2881.224 kb on + strand at 2881.224 kb on + strand at 2881.225 kb on - strand at 2881.225 kb on - strand at 2881.225 kb on - strand at 2881.225 kb on - strand at 2881.263 kb on + strand at 2881.264 kb on - strand at 2881.264 kb on - strand at 2881.267 kb on + strand at 2881.621 kb on + strand, within RR42_RS13360 at 2881.621 kb on + strand, within RR42_RS13360 at 2881.753 kb on + strand, within RR42_RS13360 at 2881.753 kb on + strand, within RR42_RS13360 at 2881.753 kb on + strand, within RR42_RS13360 at 2881.753 kb on + strand, within RR42_RS13360 at 2881.753 kb on + strand, within RR42_RS13360 at 2881.754 kb on - strand, within RR42_RS13360 at 2881.754 kb on - strand, within RR42_RS13360 at 2881.754 kb on - strand, within RR42_RS13360 at 2881.890 kb on + strand, within RR42_RS13360 at 2881.936 kb on + strand, within RR42_RS13360 at 2881.936 kb on + strand, within RR42_RS13360 at 2881.936 kb on + strand, within RR42_RS13360 at 2881.936 kb on + strand, within RR42_RS13360 at 2881.937 kb on - strand, within RR42_RS13360 at 2881.937 kb on - strand, within RR42_RS13360 at 2881.937 kb on - strand, within RR42_RS13360 at 2882.017 kb on - strand, within RR42_RS13360 at 2882.064 kb on - strand, within RR42_RS13360 at 2882.435 kb on + strand at 2882.436 kb on - strand at 2882.436 kb on - strand at 2882.501 kb on - strand, within RR42_RS13365 at 2882.591 kb on - strand, within RR42_RS13365 at 2882.741 kb on - strand, within RR42_RS13365 at 2882.992 kb on - strand at 2882.992 kb on - strand at 2883.183 kb on - strand at 2883.183 kb on - strand at 2883.210 kb on + strand at 2883.210 kb on + strand at 2883.227 kb on + strand at 2883.228 kb on - strand at 2883.229 kb on + strand at 2883.229 kb on + strand at 2883.229 kb on + strand at 2883.229 kb on + strand at 2883.230 kb on - strand at 2883.230 kb on - strand at 2883.230 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,881,145 - -0.3 2,881,159 - +0.5 2,881,160 + +1.2 2,881,160 + -0.4 2,881,160 + -1.2 2,881,161 - +0.1 2,881,161 - -1.7 2,881,224 + -0.9 2,881,224 + +1.1 2,881,225 - -1.2 2,881,225 - -0.5 2,881,225 - -0.5 2,881,225 - -1.4 2,881,263 + -0.6 2,881,264 - -1.0 2,881,264 - -2.3 2,881,267 + +1.8 2,881,621 + RR42_RS13360 0.29 -0.9 2,881,621 + RR42_RS13360 0.29 -3.3 2,881,753 + RR42_RS13360 0.43 -1.5 2,881,753 + RR42_RS13360 0.43 -0.1 2,881,753 + RR42_RS13360 0.43 -2.0 2,881,753 + RR42_RS13360 0.43 -1.3 2,881,753 + RR42_RS13360 0.43 -0.2 2,881,754 - RR42_RS13360 0.43 -1.5 2,881,754 - RR42_RS13360 0.43 -1.3 2,881,754 - RR42_RS13360 0.43 -2.4 2,881,890 + RR42_RS13360 0.58 -2.5 2,881,936 + RR42_RS13360 0.62 -3.0 2,881,936 + RR42_RS13360 0.62 -2.4 2,881,936 + RR42_RS13360 0.62 -1.1 2,881,936 + RR42_RS13360 0.62 -0.9 2,881,937 - RR42_RS13360 0.63 +0.1 2,881,937 - RR42_RS13360 0.63 -0.2 2,881,937 - RR42_RS13360 0.63 -1.9 2,882,017 - RR42_RS13360 0.71 -2.1 2,882,064 - RR42_RS13360 0.76 -1.5 2,882,435 + +0.7 2,882,436 - +0.3 2,882,436 - -0.4 2,882,501 - RR42_RS13365 0.19 -1.0 2,882,591 - RR42_RS13365 0.32 +0.5 2,882,741 - RR42_RS13365 0.54 -2.5 2,882,992 - +2.5 2,882,992 - +1.1 2,883,183 - -0.0 2,883,183 - +0.1 2,883,210 + -1.5 2,883,210 + -1.2 2,883,227 + -1.9 2,883,228 - -2.0 2,883,229 + +0.5 2,883,229 + +0.8 2,883,229 + -1.2 2,883,229 + -0.7 2,883,230 - +0.3 2,883,230 - -0.3 2,883,230 - -1.9
Or see this region's nucleotide sequence