Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12575 and RR42_RS12580 are separated by 13 nucleotides RR42_RS12580 and RR42_RS12585 are separated by 211 nucleotides RR42_RS12585 and RR42_RS12590 are separated by 256 nucleotides RR42_RS12590 and RR42_RS12595 are separated by 31 nucleotides
RR42_RS12575: RR42_RS12575 - inorganic phosphate transporter, at 2,702,692 to 2,703,702
_RS12575
RR42_RS12580: RR42_RS12580 - phosphate transport regulator, at 2,703,716 to 2,704,342
_RS12580
RR42_RS12585: RR42_RS12585 - DNA helicase, at 2,704,554 to 2,705,945
_RS12585
RR42_RS12590: RR42_RS12590 - 50S ribosomal protein L9, at 2,706,202 to 2,706,654
_RS12590
RR42_RS12595: RR42_RS12595 - 30S ribosomal protein S18, at 2,706,686 to 2,706,964
_RS12595
Position (kb)
2704
2705
2706 Strain fitness (log2 ratio)
-1
0
1
2 at 2703.795 kb on + strand, within RR42_RS12580 at 2703.796 kb on - strand, within RR42_RS12580 at 2703.956 kb on + strand, within RR42_RS12580 at 2704.299 kb on + strand at 2704.300 kb on - strand at 2704.379 kb on + strand at 2704.380 kb on - strand at 2704.380 kb on - strand at 2704.383 kb on + strand at 2704.384 kb on - strand at 2704.407 kb on - strand at 2705.958 kb on + strand at 2705.958 kb on + strand at 2705.958 kb on + strand at 2705.959 kb on - strand at 2706.049 kb on + strand at 2706.049 kb on + strand at 2706.050 kb on - strand at 2706.203 kb on + strand at 2706.203 kb on + strand at 2706.581 kb on + strand, within RR42_RS12590 at 2706.708 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,703,795 + RR42_RS12580 0.13 +1.3 2,703,796 - RR42_RS12580 0.13 +1.9 2,703,956 + RR42_RS12580 0.38 -0.2 2,704,299 + +0.1 2,704,300 - +2.1 2,704,379 + +0.3 2,704,380 - -0.3 2,704,380 - +0.5 2,704,383 + -1.5 2,704,384 - +1.4 2,704,407 - -0.3 2,705,958 + +1.1 2,705,958 + -1.2 2,705,958 + +2.2 2,705,959 - -0.1 2,706,049 + -0.9 2,706,049 + +0.7 2,706,050 - +0.5 2,706,203 + +0.9 2,706,203 + +0.5 2,706,581 + RR42_RS12590 0.84 +0.6 2,706,708 + +1.3
Or see this region's nucleotide sequence