Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12480 and RR42_RS12485 are separated by 71 nucleotides RR42_RS12485 and RR42_RS12490 overlap by 8 nucleotides RR42_RS12490 and RR42_RS12495 are separated by 50 nucleotides
RR42_RS12480: RR42_RS12480 - ABC transporter permease, at 2,684,640 to 2,685,671
_RS12480
RR42_RS12485: RR42_RS12485 - ABC transporter ATP-binding protein, at 2,685,743 to 2,686,489
_RS12485
RR42_RS12490: RR42_RS12490 - branched-chain amino acid ABC transporter substrate-binding protein, at 2,686,482 to 2,687,276
_RS12490
RR42_RS12495: RR42_RS12495 - ABC transporter permease, at 2,687,327 to 2,688,511
_RS12495
Position (kb)
2686
2687
2688 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2685.560 kb on - strand, within RR42_RS12480 at 2685.663 kb on - strand at 2685.663 kb on - strand at 2685.690 kb on - strand at 2685.697 kb on + strand at 2685.697 kb on + strand at 2685.698 kb on - strand at 2685.698 kb on - strand at 2685.911 kb on + strand, within RR42_RS12485 at 2685.912 kb on - strand, within RR42_RS12485 at 2686.218 kb on + strand, within RR42_RS12485 at 2686.537 kb on + strand at 2686.537 kb on + strand at 2686.989 kb on + strand, within RR42_RS12490 at 2686.989 kb on + strand, within RR42_RS12490 at 2686.990 kb on - strand, within RR42_RS12490 at 2687.134 kb on + strand, within RR42_RS12490 at 2687.135 kb on - strand, within RR42_RS12490 at 2687.135 kb on - strand, within RR42_RS12490 at 2687.135 kb on - strand, within RR42_RS12490 at 2687.301 kb on - strand at 2687.301 kb on - strand at 2687.301 kb on - strand at 2687.433 kb on + strand at 2687.433 kb on + strand at 2687.434 kb on - strand at 2687.634 kb on + strand, within RR42_RS12495 at 2687.634 kb on + strand, within RR42_RS12495 at 2687.685 kb on + strand, within RR42_RS12495 at 2687.686 kb on - strand, within RR42_RS12495 at 2687.691 kb on + strand, within RR42_RS12495 at 2687.692 kb on - strand, within RR42_RS12495 at 2687.692 kb on - strand, within RR42_RS12495 at 2687.692 kb on - strand, within RR42_RS12495 at 2687.692 kb on - strand, within RR42_RS12495 at 2687.724 kb on + strand, within RR42_RS12495 at 2687.775 kb on + strand, within RR42_RS12495 at 2687.922 kb on + strand, within RR42_RS12495 at 2687.922 kb on + strand, within RR42_RS12495 at 2687.922 kb on + strand, within RR42_RS12495 at 2687.923 kb on - strand, within RR42_RS12495 at 2687.994 kb on + strand, within RR42_RS12495 at 2687.994 kb on + strand, within RR42_RS12495 at 2688.093 kb on + strand, within RR42_RS12495 at 2688.093 kb on + strand, within RR42_RS12495 at 2688.094 kb on - strand, within RR42_RS12495 at 2688.094 kb on - strand, within RR42_RS12495 at 2688.241 kb on - strand, within RR42_RS12495
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,685,560 - RR42_RS12480 0.89 -0.3 2,685,663 - +0.5 2,685,663 - +0.8 2,685,690 - +2.2 2,685,697 + +2.5 2,685,697 + +0.7 2,685,698 - -0.7 2,685,698 - +0.9 2,685,911 + RR42_RS12485 0.22 -0.1 2,685,912 - RR42_RS12485 0.23 +0.2 2,686,218 + RR42_RS12485 0.64 +0.6 2,686,537 + -1.0 2,686,537 + -0.6 2,686,989 + RR42_RS12490 0.64 +0.9 2,686,989 + RR42_RS12490 0.64 +0.3 2,686,990 - RR42_RS12490 0.64 +0.3 2,687,134 + RR42_RS12490 0.82 +0.7 2,687,135 - RR42_RS12490 0.82 -0.9 2,687,135 - RR42_RS12490 0.82 -1.1 2,687,135 - RR42_RS12490 0.82 -0.1 2,687,301 - +1.5 2,687,301 - -0.8 2,687,301 - -1.4 2,687,433 + -0.5 2,687,433 + +0.1 2,687,434 - -0.1 2,687,634 + RR42_RS12495 0.26 +1.8 2,687,634 + RR42_RS12495 0.26 +1.2 2,687,685 + RR42_RS12495 0.30 -0.5 2,687,686 - RR42_RS12495 0.30 -0.9 2,687,691 + RR42_RS12495 0.31 +0.5 2,687,692 - RR42_RS12495 0.31 +0.0 2,687,692 - RR42_RS12495 0.31 -0.4 2,687,692 - RR42_RS12495 0.31 -0.1 2,687,692 - RR42_RS12495 0.31 -0.8 2,687,724 + RR42_RS12495 0.34 +3.2 2,687,775 + RR42_RS12495 0.38 -1.2 2,687,922 + RR42_RS12495 0.50 -1.4 2,687,922 + RR42_RS12495 0.50 +0.2 2,687,922 + RR42_RS12495 0.50 +1.0 2,687,923 - RR42_RS12495 0.50 +0.1 2,687,994 + RR42_RS12495 0.56 +0.4 2,687,994 + RR42_RS12495 0.56 -0.9 2,688,093 + RR42_RS12495 0.65 +1.7 2,688,093 + RR42_RS12495 0.65 +2.9 2,688,094 - RR42_RS12495 0.65 -1.4 2,688,094 - RR42_RS12495 0.65 +0.9 2,688,241 - RR42_RS12495 0.77 -0.9
Or see this region's nucleotide sequence