Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12205 and RR42_RS12210 are separated by 82 nucleotides RR42_RS12210 and RR42_RS12215 are separated by 2 nucleotides RR42_RS12215 and RR42_RS12220 are separated by 63 nucleotides
RR42_RS12205: RR42_RS12205 - alpha/beta hydrolase, at 2,625,227 to 2,626,189
_RS12205
RR42_RS12210: RR42_RS12210 - gamma-glutamyl cyclotransferase, at 2,626,272 to 2,626,673
_RS12210
RR42_RS12215: RR42_RS12215 - ribonuclease, at 2,626,676 to 2,627,173
_RS12215
RR42_RS12220: RR42_RS12220 - MFS transporter, at 2,627,237 to 2,628,514
_RS12220
Position (kb)
2626
2627
2628 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2625.676 kb on - strand, within RR42_RS12205 at 2625.676 kb on - strand, within RR42_RS12205 at 2625.787 kb on + strand, within RR42_RS12205 at 2625.787 kb on + strand, within RR42_RS12205 at 2625.787 kb on + strand, within RR42_RS12205 at 2625.788 kb on - strand, within RR42_RS12205 at 2625.788 kb on - strand, within RR42_RS12205 at 2625.788 kb on - strand, within RR42_RS12205 at 2625.788 kb on - strand, within RR42_RS12205 at 2625.788 kb on - strand, within RR42_RS12205 at 2625.788 kb on - strand, within RR42_RS12205 at 2625.789 kb on + strand, within RR42_RS12205 at 2625.790 kb on - strand, within RR42_RS12205 at 2625.790 kb on - strand, within RR42_RS12205 at 2625.790 kb on - strand, within RR42_RS12205 at 2625.790 kb on - strand, within RR42_RS12205 at 2626.002 kb on + strand, within RR42_RS12205 at 2626.002 kb on + strand, within RR42_RS12205 at 2626.002 kb on + strand, within RR42_RS12205 at 2626.003 kb on - strand, within RR42_RS12205 at 2626.005 kb on + strand, within RR42_RS12205 at 2626.006 kb on - strand, within RR42_RS12205 at 2626.006 kb on - strand, within RR42_RS12205 at 2626.215 kb on + strand at 2626.215 kb on + strand at 2626.216 kb on - strand at 2626.458 kb on + strand, within RR42_RS12210 at 2626.459 kb on - strand, within RR42_RS12210 at 2626.531 kb on + strand, within RR42_RS12210 at 2626.531 kb on + strand, within RR42_RS12210 at 2626.546 kb on + strand, within RR42_RS12210 at 2626.547 kb on - strand, within RR42_RS12210 at 2626.547 kb on - strand, within RR42_RS12210 at 2626.725 kb on + strand at 2626.725 kb on + strand at 2626.725 kb on + strand at 2626.725 kb on + strand at 2626.725 kb on + strand at 2626.726 kb on - strand, within RR42_RS12215 at 2627.094 kb on - strand, within RR42_RS12215 at 2627.094 kb on - strand, within RR42_RS12215 at 2627.161 kb on + strand at 2627.161 kb on + strand at 2627.161 kb on + strand at 2627.451 kb on + strand, within RR42_RS12220 at 2627.574 kb on - strand, within RR42_RS12220 at 2627.701 kb on + strand, within RR42_RS12220 at 2627.701 kb on + strand, within RR42_RS12220 at 2627.701 kb on + strand, within RR42_RS12220 at 2627.701 kb on + strand, within RR42_RS12220 at 2627.701 kb on + strand, within RR42_RS12220 at 2627.701 kb on + strand, within RR42_RS12220 at 2627.701 kb on + strand, within RR42_RS12220 at 2627.701 kb on + strand, within RR42_RS12220 at 2627.702 kb on - strand, within RR42_RS12220 at 2627.702 kb on - strand, within RR42_RS12220 at 2627.702 kb on - strand, within RR42_RS12220 at 2627.702 kb on - strand, within RR42_RS12220 at 2627.702 kb on - strand, within RR42_RS12220 at 2627.702 kb on - strand, within RR42_RS12220 at 2627.702 kb on - strand, within RR42_RS12220 at 2627.702 kb on - strand, within RR42_RS12220 at 2627.702 kb on - strand, within RR42_RS12220 at 2627.702 kb on - strand, within RR42_RS12220 at 2627.702 kb on - strand, within RR42_RS12220 at 2627.702 kb on - strand, within RR42_RS12220 at 2627.702 kb on - strand, within RR42_RS12220 at 2627.703 kb on + strand, within RR42_RS12220 at 2627.704 kb on - strand, within RR42_RS12220 at 2627.704 kb on - strand, within RR42_RS12220 at 2627.712 kb on + strand, within RR42_RS12220 at 2627.713 kb on - strand, within RR42_RS12220 at 2627.745 kb on + strand, within RR42_RS12220 at 2627.745 kb on + strand, within RR42_RS12220
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,625,676 - RR42_RS12205 0.47 -0.1 2,625,676 - RR42_RS12205 0.47 -0.4 2,625,787 + RR42_RS12205 0.58 +0.5 2,625,787 + RR42_RS12205 0.58 -0.5 2,625,787 + RR42_RS12205 0.58 -1.6 2,625,788 - RR42_RS12205 0.58 -0.8 2,625,788 - RR42_RS12205 0.58 +0.1 2,625,788 - RR42_RS12205 0.58 -0.9 2,625,788 - RR42_RS12205 0.58 +0.8 2,625,788 - RR42_RS12205 0.58 -0.8 2,625,788 - RR42_RS12205 0.58 -0.4 2,625,789 + RR42_RS12205 0.58 -0.8 2,625,790 - RR42_RS12205 0.58 -0.5 2,625,790 - RR42_RS12205 0.58 -2.0 2,625,790 - RR42_RS12205 0.58 -0.1 2,625,790 - RR42_RS12205 0.58 +2.5 2,626,002 + RR42_RS12205 0.80 -0.0 2,626,002 + RR42_RS12205 0.80 +0.6 2,626,002 + RR42_RS12205 0.80 -0.5 2,626,003 - RR42_RS12205 0.81 +0.2 2,626,005 + RR42_RS12205 0.81 -0.2 2,626,006 - RR42_RS12205 0.81 +0.8 2,626,006 - RR42_RS12205 0.81 -0.6 2,626,215 + -0.7 2,626,215 + +0.6 2,626,216 - +1.1 2,626,458 + RR42_RS12210 0.46 +0.4 2,626,459 - RR42_RS12210 0.47 -0.6 2,626,531 + RR42_RS12210 0.64 -0.8 2,626,531 + RR42_RS12210 0.64 +0.4 2,626,546 + RR42_RS12210 0.68 +0.4 2,626,547 - RR42_RS12210 0.68 -0.6 2,626,547 - RR42_RS12210 0.68 +0.1 2,626,725 + -1.2 2,626,725 + -1.1 2,626,725 + -1.4 2,626,725 + -0.3 2,626,725 + -0.5 2,626,726 - RR42_RS12215 0.10 +0.2 2,627,094 - RR42_RS12215 0.84 +0.8 2,627,094 - RR42_RS12215 0.84 +0.0 2,627,161 + +0.6 2,627,161 + +0.3 2,627,161 + +1.9 2,627,451 + RR42_RS12220 0.17 -1.6 2,627,574 - RR42_RS12220 0.26 +0.3 2,627,701 + RR42_RS12220 0.36 -1.1 2,627,701 + RR42_RS12220 0.36 -0.9 2,627,701 + RR42_RS12220 0.36 -1.4 2,627,701 + RR42_RS12220 0.36 -0.6 2,627,701 + RR42_RS12220 0.36 -0.5 2,627,701 + RR42_RS12220 0.36 +0.2 2,627,701 + RR42_RS12220 0.36 +0.4 2,627,701 + RR42_RS12220 0.36 -0.2 2,627,702 - RR42_RS12220 0.36 -0.1 2,627,702 - RR42_RS12220 0.36 +0.9 2,627,702 - RR42_RS12220 0.36 -0.5 2,627,702 - RR42_RS12220 0.36 +0.4 2,627,702 - RR42_RS12220 0.36 -0.9 2,627,702 - RR42_RS12220 0.36 +0.4 2,627,702 - RR42_RS12220 0.36 +0.7 2,627,702 - RR42_RS12220 0.36 -1.7 2,627,702 - RR42_RS12220 0.36 -0.8 2,627,702 - RR42_RS12220 0.36 -1.1 2,627,702 - RR42_RS12220 0.36 -1.5 2,627,702 - RR42_RS12220 0.36 -0.3 2,627,702 - RR42_RS12220 0.36 -0.1 2,627,703 + RR42_RS12220 0.36 -1.3 2,627,704 - RR42_RS12220 0.37 -0.6 2,627,704 - RR42_RS12220 0.37 -0.2 2,627,712 + RR42_RS12220 0.37 -0.9 2,627,713 - RR42_RS12220 0.37 +0.4 2,627,745 + RR42_RS12220 0.40 +0.5 2,627,745 + RR42_RS12220 0.40 -0.5
Or see this region's nucleotide sequence