Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11275 and RR42_RS11280 are separated by 382 nucleotides RR42_RS11280 and RR42_RS11285 are separated by 112 nucleotides RR42_RS11285 and RR42_RS11290 are separated by 120 nucleotides
RR42_RS11275: RR42_RS11275 - 3-methylitaconate isomerase, at 2,412,089 to 2,413,279
_RS11275
RR42_RS11280: RR42_RS11280 - phenylacetic acid degradation protein, at 2,413,662 to 2,414,090
_RS11280
RR42_RS11285: RR42_RS11285 - thioesterase, at 2,414,203 to 2,414,640
_RS11285
RR42_RS11290: RR42_RS11290 - TetR family transcriptional regulator, at 2,414,761 to 2,415,363
_RS11290
Position (kb)
2413
2414
2415 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2412.886 kb on + strand, within RR42_RS11275 at 2412.887 kb on - strand, within RR42_RS11275 at 2412.887 kb on - strand, within RR42_RS11275 at 2412.950 kb on + strand, within RR42_RS11275 at 2412.951 kb on - strand, within RR42_RS11275 at 2412.951 kb on - strand, within RR42_RS11275 at 2412.951 kb on - strand, within RR42_RS11275 at 2413.353 kb on - strand at 2413.360 kb on + strand at 2413.360 kb on + strand at 2413.361 kb on - strand at 2413.361 kb on - strand at 2413.368 kb on + strand at 2413.368 kb on + strand at 2413.368 kb on + strand at 2413.368 kb on + strand at 2413.369 kb on - strand at 2413.609 kb on + strand at 2413.610 kb on - strand at 2413.620 kb on + strand at 2413.621 kb on - strand at 2413.621 kb on - strand at 2413.621 kb on - strand at 2413.867 kb on + strand, within RR42_RS11280 at 2413.966 kb on + strand, within RR42_RS11280 at 2414.096 kb on - strand at 2414.154 kb on - strand at 2414.172 kb on - strand at 2414.172 kb on - strand at 2414.497 kb on + strand, within RR42_RS11285 at 2414.535 kb on - strand, within RR42_RS11285 at 2414.565 kb on + strand, within RR42_RS11285 at 2414.565 kb on + strand, within RR42_RS11285 at 2414.566 kb on - strand, within RR42_RS11285 at 2414.566 kb on - strand, within RR42_RS11285 at 2414.566 kb on - strand, within RR42_RS11285 at 2414.728 kb on - strand at 2414.728 kb on - strand at 2414.830 kb on + strand, within RR42_RS11290 at 2414.830 kb on + strand, within RR42_RS11290 at 2414.831 kb on - strand, within RR42_RS11290 at 2414.890 kb on + strand, within RR42_RS11290 at 2414.891 kb on - strand, within RR42_RS11290 at 2414.891 kb on - strand, within RR42_RS11290 at 2414.896 kb on + strand, within RR42_RS11290 at 2414.896 kb on + strand, within RR42_RS11290 at 2414.897 kb on - strand, within RR42_RS11290 at 2414.897 kb on - strand, within RR42_RS11290 at 2414.897 kb on - strand, within RR42_RS11290 at 2414.941 kb on + strand, within RR42_RS11290 at 2414.942 kb on - strand, within RR42_RS11290 at 2414.942 kb on - strand, within RR42_RS11290 at 2414.942 kb on - strand, within RR42_RS11290 at 2414.979 kb on - strand, within RR42_RS11290 at 2415.019 kb on + strand, within RR42_RS11290 at 2415.019 kb on + strand, within RR42_RS11290 at 2415.020 kb on - strand, within RR42_RS11290 at 2415.020 kb on - strand, within RR42_RS11290 at 2415.058 kb on + strand, within RR42_RS11290 at 2415.059 kb on - strand, within RR42_RS11290
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,412,886 + RR42_RS11275 0.67 -0.5 2,412,887 - RR42_RS11275 0.67 +0.2 2,412,887 - RR42_RS11275 0.67 +2.5 2,412,950 + RR42_RS11275 0.72 +0.9 2,412,951 - RR42_RS11275 0.72 -1.6 2,412,951 - RR42_RS11275 0.72 +0.8 2,412,951 - RR42_RS11275 0.72 +0.7 2,413,353 - -0.1 2,413,360 + +0.1 2,413,360 + +0.6 2,413,361 - +0.7 2,413,361 - -0.0 2,413,368 + -0.1 2,413,368 + +0.5 2,413,368 + -0.5 2,413,368 + -1.2 2,413,369 - -0.5 2,413,609 + -0.5 2,413,610 - -0.4 2,413,620 + +0.7 2,413,621 - -0.6 2,413,621 - +0.5 2,413,621 - -0.9 2,413,867 + RR42_RS11280 0.48 -0.6 2,413,966 + RR42_RS11280 0.71 -0.8 2,414,096 - -1.2 2,414,154 - +0.5 2,414,172 - -0.5 2,414,172 - +0.2 2,414,497 + RR42_RS11285 0.67 -1.5 2,414,535 - RR42_RS11285 0.76 -1.7 2,414,565 + RR42_RS11285 0.83 +0.8 2,414,565 + RR42_RS11285 0.83 +0.1 2,414,566 - RR42_RS11285 0.83 -1.0 2,414,566 - RR42_RS11285 0.83 +1.2 2,414,566 - RR42_RS11285 0.83 -0.8 2,414,728 - -1.5 2,414,728 - +1.2 2,414,830 + RR42_RS11290 0.11 +1.1 2,414,830 + RR42_RS11290 0.11 +0.1 2,414,831 - RR42_RS11290 0.12 -1.1 2,414,890 + RR42_RS11290 0.21 +0.3 2,414,891 - RR42_RS11290 0.22 -0.7 2,414,891 - RR42_RS11290 0.22 +1.3 2,414,896 + RR42_RS11290 0.22 +1.1 2,414,896 + RR42_RS11290 0.22 -2.0 2,414,897 - RR42_RS11290 0.23 +0.7 2,414,897 - RR42_RS11290 0.23 -0.5 2,414,897 - RR42_RS11290 0.23 +0.5 2,414,941 + RR42_RS11290 0.30 +0.5 2,414,942 - RR42_RS11290 0.30 -0.2 2,414,942 - RR42_RS11290 0.30 +0.4 2,414,942 - RR42_RS11290 0.30 -2.3 2,414,979 - RR42_RS11290 0.36 -2.1 2,415,019 + RR42_RS11290 0.43 +0.5 2,415,019 + RR42_RS11290 0.43 -1.0 2,415,020 - RR42_RS11290 0.43 -0.7 2,415,020 - RR42_RS11290 0.43 +0.9 2,415,058 + RR42_RS11290 0.49 +0.2 2,415,059 - RR42_RS11290 0.49 +0.7
Or see this region's nucleotide sequence