Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11115 and RR42_RS11120 overlap by 11 nucleotides RR42_RS11120 and RR42_RS11125 are separated by 230 nucleotides RR42_RS11125 and RR42_RS11130 are separated by 74 nucleotides RR42_RS11130 and RR42_RS11135 overlap by 1 nucleotides
RR42_RS11115: RR42_RS11115 - ornithine cyclodeaminase, at 2,374,012 to 2,374,983
_RS11115
RR42_RS11120: RR42_RS11120 - DNA-binding protein, at 2,374,973 to 2,375,614
_RS11120
RR42_RS11125: RR42_RS11125 - glycerophosphodiester phosphodiesterase, at 2,375,845 to 2,377,218
_RS11125
RR42_RS11130: RR42_RS11130 - mannosyltransferase, at 2,377,293 to 2,378,006
_RS11130
RR42_RS11135: RR42_RS11135 - branched-chain amino acid ABC transporter substrate-binding protein, at 2,378,006 to 2,378,779
_RS11135
Position (kb)
2375
2376
2377
2378 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2374.879 kb on - strand, within RR42_RS11115 at 2374.879 kb on - strand, within RR42_RS11115 at 2374.983 kb on + strand at 2374.984 kb on - strand at 2374.987 kb on + strand at 2374.987 kb on + strand at 2374.988 kb on - strand at 2374.988 kb on - strand at 2374.988 kb on - strand at 2374.988 kb on - strand at 2374.988 kb on - strand at 2374.989 kb on + strand at 2374.989 kb on + strand at 2374.990 kb on - strand at 2375.293 kb on - strand, within RR42_RS11120 at 2375.364 kb on + strand, within RR42_RS11120 at 2375.365 kb on - strand, within RR42_RS11120 at 2375.386 kb on - strand, within RR42_RS11120 at 2375.603 kb on - strand at 2375.603 kb on - strand at 2375.704 kb on + strand at 2375.705 kb on - strand at 2375.705 kb on - strand at 2375.705 kb on - strand at 2375.843 kb on - strand at 2375.843 kb on - strand at 2375.989 kb on + strand, within RR42_RS11125 at 2375.990 kb on - strand, within RR42_RS11125 at 2375.990 kb on - strand, within RR42_RS11125 at 2375.992 kb on - strand, within RR42_RS11125 at 2375.992 kb on - strand, within RR42_RS11125 at 2376.250 kb on + strand, within RR42_RS11125 at 2376.250 kb on + strand, within RR42_RS11125 at 2376.251 kb on - strand, within RR42_RS11125 at 2376.251 kb on - strand, within RR42_RS11125 at 2376.253 kb on + strand, within RR42_RS11125 at 2376.361 kb on + strand, within RR42_RS11125 at 2376.362 kb on - strand, within RR42_RS11125 at 2376.362 kb on - strand, within RR42_RS11125 at 2376.931 kb on + strand, within RR42_RS11125 at 2376.932 kb on - strand, within RR42_RS11125 at 2377.216 kb on + strand at 2377.217 kb on - strand at 2377.294 kb on + strand at 2377.295 kb on - strand at 2377.472 kb on - strand, within RR42_RS11130 at 2377.611 kb on - strand, within RR42_RS11130 at 2377.645 kb on + strand, within RR42_RS11130 at 2377.646 kb on - strand, within RR42_RS11130 at 2377.646 kb on - strand, within RR42_RS11130 at 2377.646 kb on - strand, within RR42_RS11130 at 2377.646 kb on - strand, within RR42_RS11130 at 2377.646 kb on - strand, within RR42_RS11130 at 2377.646 kb on - strand, within RR42_RS11130 at 2377.646 kb on - strand, within RR42_RS11130 at 2377.740 kb on - strand, within RR42_RS11130 at 2377.740 kb on - strand, within RR42_RS11130 at 2377.759 kb on + strand, within RR42_RS11130 at 2377.925 kb on + strand, within RR42_RS11130 at 2377.950 kb on - strand at 2377.967 kb on - strand at 2378.052 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,374,879 - RR42_RS11115 0.89 -0.1 2,374,879 - RR42_RS11115 0.89 +0.2 2,374,983 + -1.4 2,374,984 - +0.2 2,374,987 + +0.2 2,374,987 + -2.4 2,374,988 - +1.1 2,374,988 - +0.0 2,374,988 - -1.0 2,374,988 - +0.1 2,374,988 - -0.1 2,374,989 + +0.7 2,374,989 + +2.2 2,374,990 - +0.7 2,375,293 - RR42_RS11120 0.50 -0.4 2,375,364 + RR42_RS11120 0.61 +0.7 2,375,365 - RR42_RS11120 0.61 +0.3 2,375,386 - RR42_RS11120 0.64 -0.6 2,375,603 - -0.3 2,375,603 - -0.2 2,375,704 + +0.5 2,375,705 - -0.7 2,375,705 - -1.1 2,375,705 - +2.5 2,375,843 - -0.5 2,375,843 - -2.2 2,375,989 + RR42_RS11125 0.10 +0.3 2,375,990 - RR42_RS11125 0.11 -0.9 2,375,990 - RR42_RS11125 0.11 -0.2 2,375,992 - RR42_RS11125 0.11 -0.4 2,375,992 - RR42_RS11125 0.11 +0.3 2,376,250 + RR42_RS11125 0.29 +0.4 2,376,250 + RR42_RS11125 0.29 +0.5 2,376,251 - RR42_RS11125 0.30 +1.1 2,376,251 - RR42_RS11125 0.30 -0.5 2,376,253 + RR42_RS11125 0.30 +0.5 2,376,361 + RR42_RS11125 0.38 -0.4 2,376,362 - RR42_RS11125 0.38 +0.3 2,376,362 - RR42_RS11125 0.38 -1.2 2,376,931 + RR42_RS11125 0.79 -0.7 2,376,932 - RR42_RS11125 0.79 -0.6 2,377,216 + -1.0 2,377,217 - +0.2 2,377,294 + -1.0 2,377,295 - -0.0 2,377,472 - RR42_RS11130 0.25 -1.1 2,377,611 - RR42_RS11130 0.45 -1.5 2,377,645 + RR42_RS11130 0.49 -1.5 2,377,646 - RR42_RS11130 0.49 -0.7 2,377,646 - RR42_RS11130 0.49 -0.8 2,377,646 - RR42_RS11130 0.49 +0.3 2,377,646 - RR42_RS11130 0.49 -0.2 2,377,646 - RR42_RS11130 0.49 -0.3 2,377,646 - RR42_RS11130 0.49 -1.4 2,377,646 - RR42_RS11130 0.49 +0.2 2,377,740 - RR42_RS11130 0.63 -0.7 2,377,740 - RR42_RS11130 0.63 +1.5 2,377,759 + RR42_RS11130 0.65 -1.7 2,377,925 + RR42_RS11130 0.89 +0.5 2,377,950 - -0.1 2,377,967 - -0.3 2,378,052 + -0.3
Or see this region's nucleotide sequence