Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10875 and RR42_RS10880 are separated by 53 nucleotides RR42_RS10880 and RR42_RS10885 are separated by 1 nucleotides RR42_RS10885 and RR42_RS10890 are separated by 128 nucleotides RR42_RS10890 and RR42_RS10895 are separated by 82 nucleotides
RR42_RS10875: RR42_RS10875 - hypothetical protein, at 2,327,478 to 2,328,767
_RS10875
RR42_RS10880: RR42_RS10880 - ankyrin, at 2,328,821 to 2,329,510
_RS10880
RR42_RS10885: RR42_RS10885 - DNAase, at 2,329,512 to 2,330,306
_RS10885
RR42_RS10890: RR42_RS10890 - phosphinothricin acetyltransferase, at 2,330,435 to 2,330,956
_RS10890
RR42_RS10895: RR42_RS10895 - pilus assembly protein, at 2,331,039 to 2,331,422
_RS10895
Position (kb)
2329
2330
2331 Strain fitness (log2 ratio)
-2
-1
0
1 at 2328.513 kb on - strand, within RR42_RS10875 at 2328.618 kb on + strand, within RR42_RS10875 at 2328.618 kb on + strand, within RR42_RS10875 at 2328.619 kb on - strand, within RR42_RS10875 at 2328.700 kb on - strand at 2328.715 kb on - strand at 2328.817 kb on + strand at 2328.817 kb on + strand at 2328.817 kb on + strand at 2328.817 kb on + strand at 2328.817 kb on + strand at 2328.817 kb on + strand at 2328.818 kb on - strand at 2328.871 kb on - strand at 2328.871 kb on - strand at 2328.871 kb on - strand at 2329.072 kb on + strand, within RR42_RS10880 at 2329.073 kb on - strand, within RR42_RS10880 at 2329.073 kb on - strand, within RR42_RS10880 at 2329.073 kb on - strand, within RR42_RS10880 at 2329.074 kb on + strand, within RR42_RS10880 at 2329.074 kb on + strand, within RR42_RS10880 at 2329.075 kb on - strand, within RR42_RS10880 at 2329.075 kb on - strand, within RR42_RS10880 at 2329.075 kb on - strand, within RR42_RS10880 at 2329.432 kb on + strand, within RR42_RS10880 at 2329.536 kb on - strand at 2329.568 kb on + strand at 2329.681 kb on + strand, within RR42_RS10885 at 2329.682 kb on - strand, within RR42_RS10885 at 2329.784 kb on + strand, within RR42_RS10885 at 2329.785 kb on - strand, within RR42_RS10885 at 2329.785 kb on - strand, within RR42_RS10885 at 2329.785 kb on - strand, within RR42_RS10885 at 2329.956 kb on - strand, within RR42_RS10885 at 2330.110 kb on + strand, within RR42_RS10885 at 2330.111 kb on - strand, within RR42_RS10885 at 2330.138 kb on - strand, within RR42_RS10885 at 2330.244 kb on + strand at 2330.244 kb on + strand at 2330.245 kb on - strand at 2330.280 kb on + strand at 2330.281 kb on - strand at 2330.281 kb on - strand at 2330.596 kb on + strand, within RR42_RS10890 at 2330.596 kb on + strand, within RR42_RS10890 at 2330.596 kb on + strand, within RR42_RS10890 at 2330.596 kb on + strand, within RR42_RS10890 at 2330.596 kb on + strand, within RR42_RS10890 at 2330.597 kb on - strand, within RR42_RS10890 at 2330.597 kb on - strand, within RR42_RS10890 at 2330.643 kb on + strand, within RR42_RS10890 at 2330.643 kb on + strand, within RR42_RS10890 at 2330.643 kb on + strand, within RR42_RS10890 at 2330.643 kb on + strand, within RR42_RS10890 at 2330.643 kb on + strand, within RR42_RS10890 at 2330.644 kb on - strand, within RR42_RS10890 at 2330.682 kb on + strand, within RR42_RS10890 at 2330.682 kb on + strand, within RR42_RS10890 at 2330.889 kb on + strand, within RR42_RS10890 at 2330.889 kb on + strand, within RR42_RS10890 at 2330.890 kb on - strand, within RR42_RS10890 at 2330.890 kb on - strand, within RR42_RS10890 at 2330.890 kb on - strand, within RR42_RS10890 at 2330.890 kb on - strand, within RR42_RS10890 at 2330.938 kb on + strand at 2331.014 kb on - strand at 2331.017 kb on + strand at 2331.017 kb on + strand at 2331.018 kb on - strand at 2331.018 kb on - strand at 2331.208 kb on + strand, within RR42_RS10895 at 2331.208 kb on + strand, within RR42_RS10895 at 2331.209 kb on - strand, within RR42_RS10895
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,328,513 - RR42_RS10875 0.80 +0.1 2,328,618 + RR42_RS10875 0.88 -1.0 2,328,618 + RR42_RS10875 0.88 -0.5 2,328,619 - RR42_RS10875 0.88 -1.0 2,328,700 - +0.1 2,328,715 - -0.8 2,328,817 + -0.5 2,328,817 + +0.2 2,328,817 + +0.5 2,328,817 + +0.0 2,328,817 + -0.1 2,328,817 + -1.4 2,328,818 - -1.7 2,328,871 - +0.9 2,328,871 - -0.9 2,328,871 - -1.0 2,329,072 + RR42_RS10880 0.36 +0.7 2,329,073 - RR42_RS10880 0.37 -0.4 2,329,073 - RR42_RS10880 0.37 -0.4 2,329,073 - RR42_RS10880 0.37 +0.7 2,329,074 + RR42_RS10880 0.37 -0.1 2,329,074 + RR42_RS10880 0.37 -0.2 2,329,075 - RR42_RS10880 0.37 -0.4 2,329,075 - RR42_RS10880 0.37 -0.1 2,329,075 - RR42_RS10880 0.37 +0.0 2,329,432 + RR42_RS10880 0.89 +0.7 2,329,536 - -0.4 2,329,568 + -0.6 2,329,681 + RR42_RS10885 0.21 -0.1 2,329,682 - RR42_RS10885 0.21 -0.1 2,329,784 + RR42_RS10885 0.34 -1.4 2,329,785 - RR42_RS10885 0.34 -1.4 2,329,785 - RR42_RS10885 0.34 -1.1 2,329,785 - RR42_RS10885 0.34 +0.4 2,329,956 - RR42_RS10885 0.56 -2.5 2,330,110 + RR42_RS10885 0.75 -1.0 2,330,111 - RR42_RS10885 0.75 -2.6 2,330,138 - RR42_RS10885 0.79 +0.1 2,330,244 + +0.5 2,330,244 + -0.2 2,330,245 - +0.1 2,330,280 + -0.9 2,330,281 - +0.6 2,330,281 - +0.9 2,330,596 + RR42_RS10890 0.31 -0.1 2,330,596 + RR42_RS10890 0.31 +0.4 2,330,596 + RR42_RS10890 0.31 -1.5 2,330,596 + RR42_RS10890 0.31 -0.6 2,330,596 + RR42_RS10890 0.31 -0.4 2,330,597 - RR42_RS10890 0.31 -0.2 2,330,597 - RR42_RS10890 0.31 -2.2 2,330,643 + RR42_RS10890 0.40 -0.8 2,330,643 + RR42_RS10890 0.40 +1.5 2,330,643 + RR42_RS10890 0.40 +0.5 2,330,643 + RR42_RS10890 0.40 +0.4 2,330,643 + RR42_RS10890 0.40 +1.6 2,330,644 - RR42_RS10890 0.40 -0.1 2,330,682 + RR42_RS10890 0.47 -1.6 2,330,682 + RR42_RS10890 0.47 -0.3 2,330,889 + RR42_RS10890 0.87 -0.1 2,330,889 + RR42_RS10890 0.87 -2.6 2,330,890 - RR42_RS10890 0.87 +0.3 2,330,890 - RR42_RS10890 0.87 -0.6 2,330,890 - RR42_RS10890 0.87 -1.2 2,330,890 - RR42_RS10890 0.87 -0.4 2,330,938 + +0.4 2,331,014 - -0.9 2,331,017 + +0.9 2,331,017 + -0.4 2,331,018 - -1.0 2,331,018 - +0.0 2,331,208 + RR42_RS10895 0.44 +0.6 2,331,208 + RR42_RS10895 0.44 -0.5 2,331,209 - RR42_RS10895 0.44 +0.5
Or see this region's nucleotide sequence