Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10225 and RR42_RS10230 are separated by 36 nucleotides RR42_RS10230 and RR42_RS10235 are separated by 165 nucleotides
RR42_RS10225: RR42_RS10225 - nitronate monooxygenase, at 2,187,927 to 2,188,907
_RS10225
RR42_RS10230: RR42_RS10230 - hypothetical protein, at 2,188,944 to 2,190,755
_RS10230
RR42_RS10235: RR42_RS10235 - hypothetical protein, at 2,190,921 to 2,192,816
_RS10235
Position (kb)
2188
2189
2190
2191 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2188.134 kb on + strand, within RR42_RS10225 at 2188.134 kb on + strand, within RR42_RS10225 at 2188.167 kb on + strand, within RR42_RS10225 at 2188.167 kb on + strand, within RR42_RS10225 at 2188.404 kb on + strand, within RR42_RS10225 at 2188.404 kb on + strand, within RR42_RS10225 at 2188.663 kb on + strand, within RR42_RS10225 at 2188.843 kb on + strand at 2188.843 kb on + strand at 2188.844 kb on - strand at 2188.927 kb on - strand at 2189.114 kb on + strand at 2189.115 kb on - strand at 2189.194 kb on + strand, within RR42_RS10230 at 2189.414 kb on - strand, within RR42_RS10230 at 2189.470 kb on + strand, within RR42_RS10230 at 2189.471 kb on - strand, within RR42_RS10230 at 2189.586 kb on + strand, within RR42_RS10230 at 2189.754 kb on + strand, within RR42_RS10230 at 2189.770 kb on + strand, within RR42_RS10230 at 2189.771 kb on - strand, within RR42_RS10230 at 2189.788 kb on + strand, within RR42_RS10230 at 2190.137 kb on + strand, within RR42_RS10230 at 2190.138 kb on - strand, within RR42_RS10230 at 2190.546 kb on + strand, within RR42_RS10230 at 2190.549 kb on + strand, within RR42_RS10230 at 2190.651 kb on - strand at 2190.722 kb on - strand at 2190.743 kb on - strand at 2191.067 kb on + strand at 2191.067 kb on + strand at 2191.068 kb on - strand at 2191.068 kb on - strand at 2191.068 kb on - strand at 2191.235 kb on - strand, within RR42_RS10235 at 2191.297 kb on - strand, within RR42_RS10235
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,188,134 + RR42_RS10225 0.21 +0.5 2,188,134 + RR42_RS10225 0.21 -0.6 2,188,167 + RR42_RS10225 0.24 -1.0 2,188,167 + RR42_RS10225 0.24 -0.7 2,188,404 + RR42_RS10225 0.49 -1.1 2,188,404 + RR42_RS10225 0.49 -0.5 2,188,663 + RR42_RS10225 0.75 -1.1 2,188,843 + +1.0 2,188,843 + -0.7 2,188,844 - -0.9 2,188,927 - -1.3 2,189,114 + -0.8 2,189,115 - -0.5 2,189,194 + RR42_RS10230 0.14 +2.3 2,189,414 - RR42_RS10230 0.26 -0.6 2,189,470 + RR42_RS10230 0.29 -0.5 2,189,471 - RR42_RS10230 0.29 -0.0 2,189,586 + RR42_RS10230 0.35 -0.5 2,189,754 + RR42_RS10230 0.45 +0.7 2,189,770 + RR42_RS10230 0.46 -0.7 2,189,771 - RR42_RS10230 0.46 -0.5 2,189,788 + RR42_RS10230 0.47 -0.4 2,190,137 + RR42_RS10230 0.66 -0.6 2,190,138 - RR42_RS10230 0.66 -0.3 2,190,546 + RR42_RS10230 0.88 -1.3 2,190,549 + RR42_RS10230 0.89 +0.2 2,190,651 - +0.3 2,190,722 - +1.2 2,190,743 - -0.5 2,191,067 + +3.5 2,191,067 + +0.5 2,191,068 - +0.5 2,191,068 - -1.7 2,191,068 - +0.7 2,191,235 - RR42_RS10235 0.17 +0.5 2,191,297 - RR42_RS10235 0.20 +0.9
Or see this region's nucleotide sequence