Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10045 and RR42_RS10050 are separated by 294 nucleotides RR42_RS10050 and RR42_RS10055 are separated by 19 nucleotides RR42_RS10055 and RR42_RS10060 are separated by 41 nucleotides
RR42_RS10045: RR42_RS10045 - 5S ribosomal RNA, at 2,147,869 to 2,147,981
_RS10045
RR42_RS10050: RR42_RS10050 - TetR family transcriptional regulator, at 2,148,276 to 2,148,896
_RS10050
RR42_RS10055: RR42_RS10055 - TetR family transcriptional regulator, at 2,148,916 to 2,149,539
_RS10055
RR42_RS10060: RR42_RS10060 - ferredoxin:oxidoreductase FAD/NAD(P)-binding protein, at 2,149,581 to 2,150,600
_RS10060
Position (kb)
2148
2149
2150 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2148.072 kb on + strand at 2148.125 kb on + strand at 2148.125 kb on + strand at 2148.159 kb on + strand at 2148.159 kb on + strand at 2148.159 kb on + strand at 2148.160 kb on - strand at 2148.160 kb on - strand at 2148.424 kb on - strand, within RR42_RS10050 at 2148.424 kb on - strand, within RR42_RS10050 at 2148.424 kb on - strand, within RR42_RS10050 at 2148.424 kb on - strand, within RR42_RS10050 at 2148.568 kb on + strand, within RR42_RS10050 at 2148.569 kb on - strand, within RR42_RS10050 at 2148.576 kb on + strand, within RR42_RS10050 at 2148.581 kb on - strand, within RR42_RS10050 at 2148.631 kb on - strand, within RR42_RS10050 at 2148.775 kb on + strand, within RR42_RS10050 at 2148.776 kb on - strand, within RR42_RS10050 at 2148.776 kb on - strand, within RR42_RS10050 at 2148.894 kb on + strand at 2148.930 kb on - strand at 2148.975 kb on - strand at 2148.996 kb on + strand, within RR42_RS10055 at 2148.997 kb on - strand, within RR42_RS10055 at 2148.997 kb on - strand, within RR42_RS10055 at 2148.997 kb on - strand, within RR42_RS10055 at 2149.182 kb on + strand, within RR42_RS10055 at 2149.182 kb on + strand, within RR42_RS10055 at 2149.183 kb on - strand, within RR42_RS10055 at 2149.183 kb on - strand, within RR42_RS10055 at 2149.183 kb on - strand, within RR42_RS10055 at 2149.183 kb on - strand, within RR42_RS10055 at 2149.183 kb on - strand, within RR42_RS10055 at 2149.183 kb on - strand, within RR42_RS10055 at 2149.183 kb on - strand, within RR42_RS10055 at 2149.183 kb on - strand, within RR42_RS10055 at 2149.183 kb on - strand, within RR42_RS10055 at 2149.183 kb on - strand, within RR42_RS10055 at 2149.184 kb on + strand, within RR42_RS10055 at 2149.184 kb on + strand, within RR42_RS10055 at 2149.184 kb on + strand, within RR42_RS10055 at 2149.184 kb on + strand, within RR42_RS10055 at 2149.185 kb on - strand, within RR42_RS10055 at 2149.185 kb on - strand, within RR42_RS10055 at 2149.226 kb on + strand, within RR42_RS10055 at 2149.227 kb on - strand, within RR42_RS10055 at 2149.227 kb on - strand, within RR42_RS10055 at 2149.379 kb on + strand, within RR42_RS10055 at 2149.380 kb on - strand, within RR42_RS10055 at 2149.490 kb on - strand at 2149.584 kb on + strand at 2149.584 kb on + strand at 2149.584 kb on + strand at 2149.584 kb on + strand at 2149.585 kb on - strand at 2149.754 kb on - strand, within RR42_RS10060 at 2149.842 kb on + strand, within RR42_RS10060 at 2150.005 kb on + strand, within RR42_RS10060 at 2150.005 kb on + strand, within RR42_RS10060 at 2150.005 kb on + strand, within RR42_RS10060 at 2150.006 kb on - strand, within RR42_RS10060 at 2150.006 kb on - strand, within RR42_RS10060 at 2150.006 kb on - strand, within RR42_RS10060 at 2150.006 kb on - strand, within RR42_RS10060 at 2150.006 kb on - strand, within RR42_RS10060 at 2150.173 kb on + strand, within RR42_RS10060 at 2150.174 kb on - strand, within RR42_RS10060 at 2150.176 kb on + strand, within RR42_RS10060 at 2150.176 kb on + strand, within RR42_RS10060 at 2150.176 kb on + strand, within RR42_RS10060 at 2150.176 kb on + strand, within RR42_RS10060 at 2150.177 kb on - strand, within RR42_RS10060 at 2150.238 kb on - strand, within RR42_RS10060 at 2150.314 kb on - strand, within RR42_RS10060 at 2150.314 kb on - strand, within RR42_RS10060 at 2150.314 kb on - strand, within RR42_RS10060
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,148,072 + -0.7 2,148,125 + -0.6 2,148,125 + -1.5 2,148,159 + +0.2 2,148,159 + -0.5 2,148,159 + -1.5 2,148,160 - -1.1 2,148,160 - -1.8 2,148,424 - RR42_RS10050 0.24 +0.1 2,148,424 - RR42_RS10050 0.24 +0.4 2,148,424 - RR42_RS10050 0.24 -0.1 2,148,424 - RR42_RS10050 0.24 -0.1 2,148,568 + RR42_RS10050 0.47 -1.1 2,148,569 - RR42_RS10050 0.47 +1.6 2,148,576 + RR42_RS10050 0.48 -1.5 2,148,581 - RR42_RS10050 0.49 -0.8 2,148,631 - RR42_RS10050 0.57 -0.1 2,148,775 + RR42_RS10050 0.80 -0.3 2,148,776 - RR42_RS10050 0.81 +0.1 2,148,776 - RR42_RS10050 0.81 -0.6 2,148,894 + +0.4 2,148,930 - -2.1 2,148,975 - -1.7 2,148,996 + RR42_RS10055 0.13 +0.8 2,148,997 - RR42_RS10055 0.13 -0.4 2,148,997 - RR42_RS10055 0.13 -0.1 2,148,997 - RR42_RS10055 0.13 -0.1 2,149,182 + RR42_RS10055 0.43 +0.5 2,149,182 + RR42_RS10055 0.43 +0.8 2,149,183 - RR42_RS10055 0.43 -0.7 2,149,183 - RR42_RS10055 0.43 -0.2 2,149,183 - RR42_RS10055 0.43 +0.1 2,149,183 - RR42_RS10055 0.43 -1.3 2,149,183 - RR42_RS10055 0.43 -1.5 2,149,183 - RR42_RS10055 0.43 -2.3 2,149,183 - RR42_RS10055 0.43 -0.7 2,149,183 - RR42_RS10055 0.43 +0.5 2,149,183 - RR42_RS10055 0.43 -0.4 2,149,183 - RR42_RS10055 0.43 +0.1 2,149,184 + RR42_RS10055 0.43 +0.3 2,149,184 + RR42_RS10055 0.43 -0.5 2,149,184 + RR42_RS10055 0.43 +0.9 2,149,184 + RR42_RS10055 0.43 -1.2 2,149,185 - RR42_RS10055 0.43 +0.4 2,149,185 - RR42_RS10055 0.43 +0.2 2,149,226 + RR42_RS10055 0.50 -0.4 2,149,227 - RR42_RS10055 0.50 +0.6 2,149,227 - RR42_RS10055 0.50 +0.7 2,149,379 + RR42_RS10055 0.74 -0.2 2,149,380 - RR42_RS10055 0.74 -0.0 2,149,490 - -1.0 2,149,584 + +1.2 2,149,584 + -2.4 2,149,584 + -0.6 2,149,584 + +0.0 2,149,585 - -0.6 2,149,754 - RR42_RS10060 0.17 -2.0 2,149,842 + RR42_RS10060 0.26 -0.2 2,150,005 + RR42_RS10060 0.42 -0.3 2,150,005 + RR42_RS10060 0.42 +0.2 2,150,005 + RR42_RS10060 0.42 -1.4 2,150,006 - RR42_RS10060 0.42 +0.4 2,150,006 - RR42_RS10060 0.42 +1.2 2,150,006 - RR42_RS10060 0.42 -1.8 2,150,006 - RR42_RS10060 0.42 -0.9 2,150,006 - RR42_RS10060 0.42 -0.7 2,150,173 + RR42_RS10060 0.58 +0.6 2,150,174 - RR42_RS10060 0.58 +2.2 2,150,176 + RR42_RS10060 0.58 -0.8 2,150,176 + RR42_RS10060 0.58 -1.2 2,150,176 + RR42_RS10060 0.58 -0.9 2,150,176 + RR42_RS10060 0.58 +0.6 2,150,177 - RR42_RS10060 0.58 -0.9 2,150,238 - RR42_RS10060 0.64 -0.3 2,150,314 - RR42_RS10060 0.72 -0.3 2,150,314 - RR42_RS10060 0.72 -0.7 2,150,314 - RR42_RS10060 0.72 +1.3
Or see this region's nucleotide sequence