Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10005 and RR42_RS10010 are separated by 101 nucleotides RR42_RS10010 and RR42_RS10015 are separated by 81 nucleotides RR42_RS10015 and RR42_RS10020 are separated by 58 nucleotides RR42_RS10020 and RR42_RS10025 are separated by 93 nucleotides
RR42_RS10005: RR42_RS10005 - 3-oxoadipate CoA-transferase, at 2,138,007 to 2,138,657
_RS10005
RR42_RS10010: RR42_RS10010 - 3-oxoadipate CoA-transferase, at 2,138,759 to 2,139,421
_RS10010
RR42_RS10015: RR42_RS10015 - 4-methylmuconolactone transporter, at 2,139,503 to 2,140,810
_RS10015
RR42_RS10020: RR42_RS10020 - 4-methylmuconolactone methylisomerase, at 2,140,869 to 2,141,207
_RS10020
RR42_RS10025: RR42_RS10025 - muconolactone delta-isomerase, at 2,141,301 to 2,141,576
_RS10025
Position (kb)
2139
2140
2141 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2138.514 kb on + strand, within RR42_RS10005 at 2138.823 kb on - strand at 2138.823 kb on - strand at 2139.242 kb on + strand, within RR42_RS10010 at 2139.278 kb on + strand, within RR42_RS10010 at 2139.279 kb on - strand, within RR42_RS10010 at 2139.413 kb on + strand at 2139.473 kb on + strand at 2139.473 kb on + strand at 2139.474 kb on - strand at 2139.515 kb on + strand at 2139.515 kb on + strand at 2139.515 kb on + strand at 2139.516 kb on - strand at 2139.516 kb on - strand at 2139.561 kb on - strand at 2139.561 kb on - strand at 2139.728 kb on + strand, within RR42_RS10015 at 2139.807 kb on - strand, within RR42_RS10015 at 2139.807 kb on - strand, within RR42_RS10015 at 2139.843 kb on + strand, within RR42_RS10015 at 2139.843 kb on + strand, within RR42_RS10015 at 2139.844 kb on - strand, within RR42_RS10015 at 2139.844 kb on - strand, within RR42_RS10015 at 2140.158 kb on - strand, within RR42_RS10015 at 2140.186 kb on - strand, within RR42_RS10015 at 2140.186 kb on - strand, within RR42_RS10015 at 2140.214 kb on + strand, within RR42_RS10015 at 2140.215 kb on - strand, within RR42_RS10015 at 2140.287 kb on + strand, within RR42_RS10015 at 2140.287 kb on + strand, within RR42_RS10015 at 2140.288 kb on - strand, within RR42_RS10015 at 2140.409 kb on + strand, within RR42_RS10015 at 2140.409 kb on + strand, within RR42_RS10015 at 2140.410 kb on - strand, within RR42_RS10015 at 2140.537 kb on + strand, within RR42_RS10015 at 2140.537 kb on + strand, within RR42_RS10015 at 2140.538 kb on - strand, within RR42_RS10015 at 2140.662 kb on - strand, within RR42_RS10015 at 2140.662 kb on - strand, within RR42_RS10015 at 2140.662 kb on - strand, within RR42_RS10015 at 2140.839 kb on + strand at 2140.839 kb on + strand at 2140.839 kb on + strand at 2140.839 kb on + strand at 2140.839 kb on + strand at 2140.839 kb on + strand at 2140.840 kb on - strand at 2140.884 kb on + strand at 2140.983 kb on + strand, within RR42_RS10020 at 2140.983 kb on + strand, within RR42_RS10020 at 2141.091 kb on + strand, within RR42_RS10020 at 2141.100 kb on + strand, within RR42_RS10020 at 2141.101 kb on - strand, within RR42_RS10020 at 2141.101 kb on - strand, within RR42_RS10020 at 2141.101 kb on - strand, within RR42_RS10020 at 2141.307 kb on + strand at 2141.428 kb on + strand, within RR42_RS10025 at 2141.428 kb on + strand, within RR42_RS10025 at 2141.428 kb on + strand, within RR42_RS10025 at 2141.428 kb on + strand, within RR42_RS10025 at 2141.429 kb on - strand, within RR42_RS10025 at 2141.429 kb on - strand, within RR42_RS10025 at 2141.449 kb on - strand, within RR42_RS10025 at 2141.526 kb on + strand, within RR42_RS10025 at 2141.526 kb on + strand, within RR42_RS10025 at 2141.527 kb on - strand, within RR42_RS10025 at 2141.527 kb on - strand, within RR42_RS10025 at 2141.528 kb on + strand, within RR42_RS10025 at 2141.528 kb on + strand, within RR42_RS10025 at 2141.528 kb on + strand, within RR42_RS10025 at 2141.528 kb on + strand, within RR42_RS10025 at 2141.528 kb on + strand, within RR42_RS10025 at 2141.529 kb on - strand, within RR42_RS10025 at 2141.529 kb on - strand, within RR42_RS10025 at 2141.529 kb on - strand, within RR42_RS10025 at 2141.529 kb on - strand, within RR42_RS10025 at 2141.529 kb on - strand, within RR42_RS10025 at 2141.529 kb on - strand, within RR42_RS10025 at 2141.668 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,138,514 + RR42_RS10005 0.78 +0.7 2,138,823 - -1.2 2,138,823 - -1.5 2,139,242 + RR42_RS10010 0.73 +0.2 2,139,278 + RR42_RS10010 0.78 +0.7 2,139,279 - RR42_RS10010 0.78 -0.6 2,139,413 + -1.2 2,139,473 + +0.3 2,139,473 + +0.2 2,139,474 - +0.2 2,139,515 + +1.9 2,139,515 + -1.3 2,139,515 + -0.5 2,139,516 - -2.5 2,139,516 - +0.3 2,139,561 - +0.4 2,139,561 - -0.1 2,139,728 + RR42_RS10015 0.17 -0.1 2,139,807 - RR42_RS10015 0.23 -0.8 2,139,807 - RR42_RS10015 0.23 +1.5 2,139,843 + RR42_RS10015 0.26 -0.0 2,139,843 + RR42_RS10015 0.26 -1.7 2,139,844 - RR42_RS10015 0.26 +0.5 2,139,844 - RR42_RS10015 0.26 -0.3 2,140,158 - RR42_RS10015 0.50 +0.2 2,140,186 - RR42_RS10015 0.52 -1.2 2,140,186 - RR42_RS10015 0.52 +1.0 2,140,214 + RR42_RS10015 0.54 +0.1 2,140,215 - RR42_RS10015 0.54 -0.3 2,140,287 + RR42_RS10015 0.60 +0.3 2,140,287 + RR42_RS10015 0.60 +0.7 2,140,288 - RR42_RS10015 0.60 +0.9 2,140,409 + RR42_RS10015 0.69 +0.6 2,140,409 + RR42_RS10015 0.69 -0.9 2,140,410 - RR42_RS10015 0.69 +0.0 2,140,537 + RR42_RS10015 0.79 +1.4 2,140,537 + RR42_RS10015 0.79 -0.6 2,140,538 - RR42_RS10015 0.79 -1.6 2,140,662 - RR42_RS10015 0.89 -0.1 2,140,662 - RR42_RS10015 0.89 +0.2 2,140,662 - RR42_RS10015 0.89 +0.5 2,140,839 + +0.5 2,140,839 + -0.9 2,140,839 + -0.4 2,140,839 + +0.4 2,140,839 + +0.8 2,140,839 + -2.5 2,140,840 - -1.6 2,140,884 + +0.2 2,140,983 + RR42_RS10020 0.34 +0.1 2,140,983 + RR42_RS10020 0.34 -1.7 2,141,091 + RR42_RS10020 0.65 -1.1 2,141,100 + RR42_RS10020 0.68 +1.1 2,141,101 - RR42_RS10020 0.68 -1.7 2,141,101 - RR42_RS10020 0.68 +2.1 2,141,101 - RR42_RS10020 0.68 -0.9 2,141,307 + +0.1 2,141,428 + RR42_RS10025 0.46 -0.1 2,141,428 + RR42_RS10025 0.46 -1.1 2,141,428 + RR42_RS10025 0.46 +0.9 2,141,428 + RR42_RS10025 0.46 +0.2 2,141,429 - RR42_RS10025 0.46 +1.8 2,141,429 - RR42_RS10025 0.46 +2.5 2,141,449 - RR42_RS10025 0.54 +1.3 2,141,526 + RR42_RS10025 0.82 -0.0 2,141,526 + RR42_RS10025 0.82 -0.2 2,141,527 - RR42_RS10025 0.82 -1.3 2,141,527 - RR42_RS10025 0.82 +0.5 2,141,528 + RR42_RS10025 0.82 -1.0 2,141,528 + RR42_RS10025 0.82 -0.1 2,141,528 + RR42_RS10025 0.82 -1.3 2,141,528 + RR42_RS10025 0.82 -2.1 2,141,528 + RR42_RS10025 0.82 -0.2 2,141,529 - RR42_RS10025 0.83 +0.9 2,141,529 - RR42_RS10025 0.83 +0.2 2,141,529 - RR42_RS10025 0.83 +0.9 2,141,529 - RR42_RS10025 0.83 -0.7 2,141,529 - RR42_RS10025 0.83 +0.8 2,141,529 - RR42_RS10025 0.83 -0.6 2,141,668 + +1.5
Or see this region's nucleotide sequence