Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS09420 and RR42_RS09425 are separated by 41 nucleotides RR42_RS09425 and RR42_RS09430 are separated by 102 nucleotides
RR42_RS09420: RR42_RS09420 - TetR family transcriptional regulator, at 1,998,786 to 1,999,484
_RS09420
RR42_RS09425: RR42_RS09425 - carotenoid oxygenase, at 1,999,526 to 2,000,914
_RS09425
RR42_RS09430: RR42_RS09430 - hypothetical protein, at 2,001,017 to 2,002,525
_RS09430
Position (kb)
1999
2000
2001 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1998.553 kb on + strand at 1998.553 kb on + strand at 1998.553 kb on + strand at 1998.554 kb on - strand at 1998.554 kb on - strand at 1998.554 kb on - strand at 1998.554 kb on - strand at 1998.554 kb on - strand at 1998.564 kb on + strand at 1998.564 kb on + strand at 1998.565 kb on - strand at 1998.565 kb on - strand at 1999.140 kb on + strand, within RR42_RS09420 at 1999.140 kb on + strand, within RR42_RS09420 at 1999.140 kb on + strand, within RR42_RS09420 at 1999.141 kb on - strand, within RR42_RS09420 at 1999.279 kb on + strand, within RR42_RS09420 at 1999.280 kb on - strand, within RR42_RS09420 at 1999.482 kb on + strand at 1999.583 kb on + strand at 1999.583 kb on + strand at 1999.627 kb on + strand at 1999.848 kb on + strand, within RR42_RS09425 at 1999.848 kb on + strand, within RR42_RS09425 at 1999.849 kb on - strand, within RR42_RS09425 at 1999.849 kb on - strand, within RR42_RS09425 at 1999.935 kb on + strand, within RR42_RS09425 at 1999.936 kb on - strand, within RR42_RS09425 at 1999.936 kb on - strand, within RR42_RS09425 at 2000.046 kb on + strand, within RR42_RS09425 at 2000.046 kb on + strand, within RR42_RS09425 at 2000.047 kb on - strand, within RR42_RS09425 at 2000.047 kb on - strand, within RR42_RS09425 at 2000.112 kb on + strand, within RR42_RS09425 at 2000.112 kb on + strand, within RR42_RS09425 at 2000.112 kb on + strand, within RR42_RS09425 at 2000.112 kb on + strand, within RR42_RS09425 at 2000.112 kb on + strand, within RR42_RS09425 at 2000.113 kb on - strand, within RR42_RS09425 at 2000.323 kb on + strand, within RR42_RS09425 at 2000.324 kb on - strand, within RR42_RS09425 at 2000.533 kb on + strand, within RR42_RS09425 at 2000.534 kb on - strand, within RR42_RS09425 at 2000.779 kb on - strand at 2000.870 kb on - strand at 2000.917 kb on - strand at 2000.971 kb on - strand at 2000.971 kb on - strand at 2000.971 kb on - strand at 2000.971 kb on - strand at 2000.971 kb on - strand at 2001.132 kb on + strand at 2001.133 kb on - strand at 2001.309 kb on + strand, within RR42_RS09430 at 2001.313 kb on + strand, within RR42_RS09430 at 2001.313 kb on + strand, within RR42_RS09430 at 2001.313 kb on + strand, within RR42_RS09430 at 2001.314 kb on - strand, within RR42_RS09430 at 2001.314 kb on - strand, within RR42_RS09430 at 2001.384 kb on + strand, within RR42_RS09430 at 2001.453 kb on + strand, within RR42_RS09430 at 2001.486 kb on + strand, within RR42_RS09430 at 2001.487 kb on - strand, within RR42_RS09430 at 2001.618 kb on + strand, within RR42_RS09430 at 2001.619 kb on - strand, within RR42_RS09430 at 2001.640 kb on - strand, within RR42_RS09430 at 2001.640 kb on - strand, within RR42_RS09430 at 2001.787 kb on - strand, within RR42_RS09430 at 2001.787 kb on - strand, within RR42_RS09430 at 2001.787 kb on - strand, within RR42_RS09430 at 2001.804 kb on + strand, within RR42_RS09430 at 2001.804 kb on + strand, within RR42_RS09430 at 2001.805 kb on - strand, within RR42_RS09430 at 2001.805 kb on - strand, within RR42_RS09430 at 2001.805 kb on - strand, within RR42_RS09430 at 2001.909 kb on + strand, within RR42_RS09430
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,998,553 + -0.3 1,998,553 + +0.4 1,998,553 + -0.1 1,998,554 - +0.2 1,998,554 - -3.0 1,998,554 - +0.8 1,998,554 - +1.3 1,998,554 - +0.7 1,998,564 + +0.8 1,998,564 + -0.5 1,998,565 - +0.5 1,998,565 - +0.5 1,999,140 + RR42_RS09420 0.51 +1.7 1,999,140 + RR42_RS09420 0.51 +1.9 1,999,140 + RR42_RS09420 0.51 -3.5 1,999,141 - RR42_RS09420 0.51 +1.1 1,999,279 + RR42_RS09420 0.71 -0.9 1,999,280 - RR42_RS09420 0.71 +0.9 1,999,482 + +1.8 1,999,583 + +0.8 1,999,583 + -0.0 1,999,627 + -0.3 1,999,848 + RR42_RS09425 0.23 -1.0 1,999,848 + RR42_RS09425 0.23 -1.9 1,999,849 - RR42_RS09425 0.23 +0.2 1,999,849 - RR42_RS09425 0.23 -0.5 1,999,935 + RR42_RS09425 0.29 +0.2 1,999,936 - RR42_RS09425 0.30 -1.6 1,999,936 - RR42_RS09425 0.30 +0.5 2,000,046 + RR42_RS09425 0.37 +0.5 2,000,046 + RR42_RS09425 0.37 -0.4 2,000,047 - RR42_RS09425 0.38 -0.9 2,000,047 - RR42_RS09425 0.38 -2.0 2,000,112 + RR42_RS09425 0.42 -0.2 2,000,112 + RR42_RS09425 0.42 -0.3 2,000,112 + RR42_RS09425 0.42 -0.6 2,000,112 + RR42_RS09425 0.42 +0.0 2,000,112 + RR42_RS09425 0.42 -0.5 2,000,113 - RR42_RS09425 0.42 +0.3 2,000,323 + RR42_RS09425 0.57 +0.8 2,000,324 - RR42_RS09425 0.57 -0.2 2,000,533 + RR42_RS09425 0.72 +0.5 2,000,534 - RR42_RS09425 0.73 -0.1 2,000,779 - -1.0 2,000,870 - +1.6 2,000,917 - -1.5 2,000,971 - -0.3 2,000,971 - -0.1 2,000,971 - -0.9 2,000,971 - +0.3 2,000,971 - +0.3 2,001,132 + +0.2 2,001,133 - +0.3 2,001,309 + RR42_RS09430 0.19 +2.2 2,001,313 + RR42_RS09430 0.20 +0.7 2,001,313 + RR42_RS09430 0.20 +0.8 2,001,313 + RR42_RS09430 0.20 -2.2 2,001,314 - RR42_RS09430 0.20 -0.4 2,001,314 - RR42_RS09430 0.20 -0.9 2,001,384 + RR42_RS09430 0.24 -0.1 2,001,453 + RR42_RS09430 0.29 +0.3 2,001,486 + RR42_RS09430 0.31 -1.2 2,001,487 - RR42_RS09430 0.31 -0.2 2,001,618 + RR42_RS09430 0.40 -2.1 2,001,619 - RR42_RS09430 0.40 +1.5 2,001,640 - RR42_RS09430 0.41 +0.3 2,001,640 - RR42_RS09430 0.41 +0.2 2,001,787 - RR42_RS09430 0.51 -1.9 2,001,787 - RR42_RS09430 0.51 -1.1 2,001,787 - RR42_RS09430 0.51 -1.2 2,001,804 + RR42_RS09430 0.52 +0.8 2,001,804 + RR42_RS09430 0.52 -1.3 2,001,805 - RR42_RS09430 0.52 +0.2 2,001,805 - RR42_RS09430 0.52 -1.1 2,001,805 - RR42_RS09430 0.52 +0.5 2,001,909 + RR42_RS09430 0.59 -0.4
Or see this region's nucleotide sequence