Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS09330 and RR42_RS09335 are separated by 336 nucleotides RR42_RS09335 and RR42_RS09340 are separated by 100 nucleotides RR42_RS09340 and RR42_RS09345 are separated by 141 nucleotides
RR42_RS09330: RR42_RS09330 - 3-oxoacyl-ACP reductase, at 1,972,973 to 1,973,773
_RS09330
RR42_RS09335: RR42_RS09335 - DNA polymerase II, at 1,974,110 to 1,976,485
_RS09335
RR42_RS09340: RR42_RS09340 - lysine transporter LysE, at 1,976,586 to 1,977,218
_RS09340
RR42_RS09345: RR42_RS09345 - limonene-1,2-epoxide hydrolase, at 1,977,360 to 1,977,803
_RS09345
Position (kb)
1974
1975
1976
1977 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1973.432 kb on + strand, within RR42_RS09330 at 1973.433 kb on - strand, within RR42_RS09330 at 1973.433 kb on - strand, within RR42_RS09330 at 1973.510 kb on + strand, within RR42_RS09330 at 1973.510 kb on + strand, within RR42_RS09330 at 1973.511 kb on - strand, within RR42_RS09330 at 1973.511 kb on - strand, within RR42_RS09330 at 1973.511 kb on - strand, within RR42_RS09330 at 1973.511 kb on - strand, within RR42_RS09330 at 1973.511 kb on - strand, within RR42_RS09330 at 1973.717 kb on + strand at 1973.717 kb on + strand at 1973.801 kb on - strand at 1973.801 kb on - strand at 1973.801 kb on - strand at 1973.835 kb on - strand at 1974.073 kb on - strand at 1974.359 kb on + strand, within RR42_RS09335 at 1974.359 kb on + strand, within RR42_RS09335 at 1974.425 kb on + strand, within RR42_RS09335 at 1974.426 kb on - strand, within RR42_RS09335 at 1974.563 kb on + strand, within RR42_RS09335 at 1974.662 kb on + strand, within RR42_RS09335 at 1974.662 kb on + strand, within RR42_RS09335 at 1974.663 kb on - strand, within RR42_RS09335 at 1974.663 kb on - strand, within RR42_RS09335 at 1974.663 kb on - strand, within RR42_RS09335 at 1974.663 kb on - strand, within RR42_RS09335 at 1974.664 kb on + strand, within RR42_RS09335 at 1974.665 kb on - strand, within RR42_RS09335 at 1974.665 kb on - strand, within RR42_RS09335 at 1974.665 kb on - strand, within RR42_RS09335 at 1974.717 kb on - strand, within RR42_RS09335 at 1974.846 kb on - strand, within RR42_RS09335 at 1974.958 kb on + strand, within RR42_RS09335 at 1975.029 kb on + strand, within RR42_RS09335 at 1975.029 kb on + strand, within RR42_RS09335 at 1975.030 kb on - strand, within RR42_RS09335 at 1975.118 kb on + strand, within RR42_RS09335 at 1975.256 kb on + strand, within RR42_RS09335 at 1975.256 kb on + strand, within RR42_RS09335 at 1975.257 kb on - strand, within RR42_RS09335 at 1975.257 kb on - strand, within RR42_RS09335 at 1975.257 kb on - strand, within RR42_RS09335 at 1975.283 kb on + strand, within RR42_RS09335 at 1975.398 kb on - strand, within RR42_RS09335 at 1975.626 kb on - strand, within RR42_RS09335 at 1975.754 kb on + strand, within RR42_RS09335 at 1975.754 kb on + strand, within RR42_RS09335 at 1976.025 kb on - strand, within RR42_RS09335 at 1976.025 kb on - strand, within RR42_RS09335 at 1976.025 kb on - strand, within RR42_RS09335 at 1976.127 kb on - strand, within RR42_RS09335 at 1976.138 kb on + strand, within RR42_RS09335 at 1976.138 kb on + strand, within RR42_RS09335 at 1976.139 kb on - strand, within RR42_RS09335 at 1976.217 kb on - strand, within RR42_RS09335 at 1976.321 kb on + strand at 1976.322 kb on - strand at 1976.322 kb on - strand at 1976.602 kb on + strand at 1976.603 kb on - strand at 1976.603 kb on - strand at 1976.777 kb on + strand, within RR42_RS09340 at 1976.783 kb on + strand, within RR42_RS09340 at 1976.783 kb on + strand, within RR42_RS09340 at 1976.784 kb on - strand, within RR42_RS09340 at 1976.804 kb on + strand, within RR42_RS09340 at 1976.827 kb on + strand, within RR42_RS09340 at 1976.828 kb on - strand, within RR42_RS09340 at 1976.828 kb on - strand, within RR42_RS09340 at 1976.828 kb on - strand, within RR42_RS09340 at 1976.859 kb on - strand, within RR42_RS09340 at 1976.865 kb on - strand, within RR42_RS09340 at 1976.963 kb on - strand, within RR42_RS09340 at 1976.963 kb on - strand, within RR42_RS09340 at 1976.963 kb on - strand, within RR42_RS09340 at 1976.972 kb on - strand, within RR42_RS09340 at 1977.008 kb on - strand, within RR42_RS09340 at 1977.059 kb on - strand, within RR42_RS09340 at 1977.239 kb on - strand at 1977.242 kb on + strand at 1977.242 kb on + strand at 1977.242 kb on + strand at 1977.242 kb on + strand at 1977.293 kb on + strand at 1977.294 kb on - strand at 1977.329 kb on + strand at 1977.344 kb on + strand at 1977.345 kb on - strand at 1977.387 kb on - strand at 1977.418 kb on + strand, within RR42_RS09345 at 1977.418 kb on + strand, within RR42_RS09345 at 1977.419 kb on - strand, within RR42_RS09345 at 1977.419 kb on - strand, within RR42_RS09345 at 1977.419 kb on - strand, within RR42_RS09345 at 1977.419 kb on - strand, within RR42_RS09345 at 1977.419 kb on - strand, within RR42_RS09345
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,973,432 + RR42_RS09330 0.57 -3.6 1,973,433 - RR42_RS09330 0.57 +0.1 1,973,433 - RR42_RS09330 0.57 +0.5 1,973,510 + RR42_RS09330 0.67 +1.5 1,973,510 + RR42_RS09330 0.67 -0.4 1,973,511 - RR42_RS09330 0.67 +0.6 1,973,511 - RR42_RS09330 0.67 -0.6 1,973,511 - RR42_RS09330 0.67 -0.9 1,973,511 - RR42_RS09330 0.67 +0.5 1,973,511 - RR42_RS09330 0.67 +0.2 1,973,717 + -0.1 1,973,717 + -0.5 1,973,801 - -0.5 1,973,801 - +0.7 1,973,801 - -0.1 1,973,835 - -1.5 1,974,073 - -2.1 1,974,359 + RR42_RS09335 0.10 -1.1 1,974,359 + RR42_RS09335 0.10 -0.2 1,974,425 + RR42_RS09335 0.13 -0.5 1,974,426 - RR42_RS09335 0.13 -0.1 1,974,563 + RR42_RS09335 0.19 -1.2 1,974,662 + RR42_RS09335 0.23 +0.5 1,974,662 + RR42_RS09335 0.23 +0.2 1,974,663 - RR42_RS09335 0.23 -0.1 1,974,663 - RR42_RS09335 0.23 -0.7 1,974,663 - RR42_RS09335 0.23 -2.0 1,974,663 - RR42_RS09335 0.23 -0.2 1,974,664 + RR42_RS09335 0.23 -0.6 1,974,665 - RR42_RS09335 0.23 +0.3 1,974,665 - RR42_RS09335 0.23 -1.2 1,974,665 - RR42_RS09335 0.23 +0.5 1,974,717 - RR42_RS09335 0.26 +0.4 1,974,846 - RR42_RS09335 0.31 +0.2 1,974,958 + RR42_RS09335 0.36 -2.2 1,975,029 + RR42_RS09335 0.39 -1.3 1,975,029 + RR42_RS09335 0.39 +0.3 1,975,030 - RR42_RS09335 0.39 -0.7 1,975,118 + RR42_RS09335 0.42 +1.5 1,975,256 + RR42_RS09335 0.48 -1.5 1,975,256 + RR42_RS09335 0.48 -1.2 1,975,257 - RR42_RS09335 0.48 +0.1 1,975,257 - RR42_RS09335 0.48 +0.9 1,975,257 - RR42_RS09335 0.48 +0.7 1,975,283 + RR42_RS09335 0.49 -0.3 1,975,398 - RR42_RS09335 0.54 +0.6 1,975,626 - RR42_RS09335 0.64 +0.1 1,975,754 + RR42_RS09335 0.69 -0.3 1,975,754 + RR42_RS09335 0.69 -1.9 1,976,025 - RR42_RS09335 0.81 +0.3 1,976,025 - RR42_RS09335 0.81 -0.9 1,976,025 - RR42_RS09335 0.81 -0.0 1,976,127 - RR42_RS09335 0.85 -0.8 1,976,138 + RR42_RS09335 0.85 -0.1 1,976,138 + RR42_RS09335 0.85 +0.5 1,976,139 - RR42_RS09335 0.85 -0.1 1,976,217 - RR42_RS09335 0.89 +0.4 1,976,321 + +0.3 1,976,322 - +0.8 1,976,322 - -0.9 1,976,602 + -0.2 1,976,603 - -1.1 1,976,603 - +0.5 1,976,777 + RR42_RS09340 0.30 -0.3 1,976,783 + RR42_RS09340 0.31 -0.7 1,976,783 + RR42_RS09340 0.31 -1.4 1,976,784 - RR42_RS09340 0.31 -0.1 1,976,804 + RR42_RS09340 0.34 +1.1 1,976,827 + RR42_RS09340 0.38 -1.1 1,976,828 - RR42_RS09340 0.38 -1.1 1,976,828 - RR42_RS09340 0.38 -0.7 1,976,828 - RR42_RS09340 0.38 -0.6 1,976,859 - RR42_RS09340 0.43 -0.7 1,976,865 - RR42_RS09340 0.44 -0.9 1,976,963 - RR42_RS09340 0.60 +0.5 1,976,963 - RR42_RS09340 0.60 -1.1 1,976,963 - RR42_RS09340 0.60 -0.1 1,976,972 - RR42_RS09340 0.61 +1.3 1,977,008 - RR42_RS09340 0.67 -0.9 1,977,059 - RR42_RS09340 0.75 -0.2 1,977,239 - +1.5 1,977,242 + +0.6 1,977,242 + -0.9 1,977,242 + -0.4 1,977,242 + -1.5 1,977,293 + -0.2 1,977,294 - +0.2 1,977,329 + +0.2 1,977,344 + -0.5 1,977,345 - +2.1 1,977,387 - -0.3 1,977,418 + RR42_RS09345 0.13 -1.2 1,977,418 + RR42_RS09345 0.13 +2.5 1,977,419 - RR42_RS09345 0.13 +0.5 1,977,419 - RR42_RS09345 0.13 -0.5 1,977,419 - RR42_RS09345 0.13 -0.2 1,977,419 - RR42_RS09345 0.13 -0.1 1,977,419 - RR42_RS09345 0.13 +0.5
Or see this region's nucleotide sequence