Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS09095 and RR42_RS09100 are separated by 306 nucleotides RR42_RS09100 and RR42_RS09105 are separated by 105 nucleotides
RR42_RS09095: RR42_RS09095 - amidase, at 1,913,618 to 1,914,961
_RS09095
RR42_RS09100: RR42_RS09100 - transcriptional regulator, at 1,915,268 to 1,915,888
_RS09100
RR42_RS09105: RR42_RS09105 - 5-oxoprolinase, at 1,915,994 to 1,919,644
_RS09105
Position (kb)
1915
1916 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1914.341 kb on - strand, within RR42_RS09095 at 1914.342 kb on + strand, within RR42_RS09095 at 1914.342 kb on + strand, within RR42_RS09095 at 1914.342 kb on + strand, within RR42_RS09095 at 1914.943 kb on + strand at 1914.943 kb on + strand at 1914.943 kb on + strand at 1914.943 kb on + strand at 1914.944 kb on - strand at 1914.944 kb on - strand at 1914.944 kb on - strand at 1914.944 kb on - strand at 1915.140 kb on + strand at 1915.171 kb on - strand at 1915.171 kb on - strand at 1915.188 kb on - strand at 1915.188 kb on - strand at 1915.188 kb on - strand at 1915.216 kb on + strand at 1915.216 kb on + strand at 1915.216 kb on + strand at 1915.217 kb on - strand at 1915.240 kb on + strand at 1915.241 kb on - strand at 1915.241 kb on - strand at 1915.241 kb on - strand at 1915.276 kb on + strand at 1915.396 kb on + strand, within RR42_RS09100 at 1915.473 kb on - strand, within RR42_RS09100 at 1915.481 kb on + strand, within RR42_RS09100 at 1915.482 kb on - strand, within RR42_RS09100 at 1915.598 kb on - strand, within RR42_RS09100 at 1915.640 kb on + strand, within RR42_RS09100 at 1915.640 kb on + strand, within RR42_RS09100 at 1915.641 kb on - strand, within RR42_RS09100 at 1915.706 kb on + strand, within RR42_RS09100 at 1915.706 kb on + strand, within RR42_RS09100 at 1915.706 kb on + strand, within RR42_RS09100 at 1915.707 kb on - strand, within RR42_RS09100 at 1915.707 kb on - strand, within RR42_RS09100 at 1915.707 kb on - strand, within RR42_RS09100 at 1915.853 kb on + strand at 1915.854 kb on - strand at 1915.886 kb on + strand at 1915.887 kb on - strand at 1915.887 kb on - strand at 1915.993 kb on + strand at 1915.994 kb on - strand at 1916.150 kb on + strand at 1916.151 kb on - strand at 1916.393 kb on + strand, within RR42_RS09105
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,914,341 - RR42_RS09095 0.54 -1.3 1,914,342 + RR42_RS09095 0.54 -0.5 1,914,342 + RR42_RS09095 0.54 -1.3 1,914,342 + RR42_RS09095 0.54 +0.2 1,914,943 + -0.1 1,914,943 + -0.7 1,914,943 + +1.4 1,914,943 + -0.4 1,914,944 - -0.7 1,914,944 - +0.5 1,914,944 - -0.2 1,914,944 - -0.7 1,915,140 + +0.2 1,915,171 - +1.7 1,915,171 - -0.1 1,915,188 - -0.0 1,915,188 - +0.3 1,915,188 - -0.1 1,915,216 + -0.5 1,915,216 + -0.5 1,915,216 + +0.5 1,915,217 - -1.7 1,915,240 + +0.6 1,915,241 - +0.0 1,915,241 - -0.5 1,915,241 - +0.5 1,915,276 + +1.3 1,915,396 + RR42_RS09100 0.21 -3.0 1,915,473 - RR42_RS09100 0.33 -0.0 1,915,481 + RR42_RS09100 0.34 -1.3 1,915,482 - RR42_RS09100 0.34 -0.9 1,915,598 - RR42_RS09100 0.53 -0.6 1,915,640 + RR42_RS09100 0.60 -1.8 1,915,640 + RR42_RS09100 0.60 -0.1 1,915,641 - RR42_RS09100 0.60 -1.2 1,915,706 + RR42_RS09100 0.71 +1.8 1,915,706 + RR42_RS09100 0.71 -0.5 1,915,706 + RR42_RS09100 0.71 +1.1 1,915,707 - RR42_RS09100 0.71 +0.7 1,915,707 - RR42_RS09100 0.71 +2.0 1,915,707 - RR42_RS09100 0.71 -0.3 1,915,853 + +0.8 1,915,854 - +0.3 1,915,886 + +1.4 1,915,887 - -0.0 1,915,887 - -0.1 1,915,993 + +0.8 1,915,994 - -0.1 1,916,150 + -0.1 1,916,151 - +0.0 1,916,393 + RR42_RS09105 0.11 +1.5
Or see this region's nucleotide sequence