Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS08795 and RR42_RS08800 are separated by 140 nucleotides RR42_RS08800 and RR42_RS08805 are separated by 52 nucleotides RR42_RS08805 and RR42_RS08810 are separated by 10 nucleotides RR42_RS08810 and RR42_RS08815 are separated by 106 nucleotides
RR42_RS08795: RR42_RS08795 - molybdopterin-guanine dinucleotide biosynthesis protein B, at 1,842,850 to 1,843,428
_RS08795
RR42_RS08800: RR42_RS08800 - peptidylprolyl isomerase, at 1,843,569 to 1,844,315
_RS08800
RR42_RS08805: RR42_RS08805 - nitrate reductase, at 1,844,368 to 1,845,051
_RS08805
RR42_RS08810: RR42_RS08810 - nitrate reductase, at 1,845,062 to 1,845,766
_RS08810
RR42_RS08815: RR42_RS08815 - nitrate reductase, at 1,845,873 to 1,847,426
_RS08815
Position (kb) 1844
1845
1846 Strain fitness (log2 ratio) -1
0
1 at 1843.769 kb on - strand, within RR42_RS08800 at 1844.250 kb on - strand at 1844.305 kb on - strand at 1844.520 kb on + strand, within RR42_RS08805 at 1844.521 kb on - strand, within RR42_RS08805 at 1844.522 kb on + strand, within RR42_RS08805 at 1844.523 kb on - strand, within RR42_RS08805 at 1845.011 kb on + strand at 1845.012 kb on - strand at 1845.012 kb on - strand at 1845.012 kb on - strand at 1845.012 kb on - strand at 1845.012 kb on - strand at 1845.017 kb on + strand at 1845.307 kb on - strand, within RR42_RS08810 at 1845.441 kb on + strand, within RR42_RS08810 at 1845.442 kb on - strand, within RR42_RS08810 at 1845.487 kb on - strand, within RR42_RS08810 at 1845.594 kb on + strand, within RR42_RS08810 at 1845.594 kb on + strand, within RR42_RS08810 at 1845.594 kb on + strand, within RR42_RS08810 at 1845.595 kb on - strand, within RR42_RS08810
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,843,769 - RR42_RS08800 0.27 -1.6 1,844,250 - +0.8 1,844,305 - -0.3 1,844,520 + RR42_RS08805 0.22 -1.5 1,844,521 - RR42_RS08805 0.22 -1.3 1,844,522 + RR42_RS08805 0.23 -0.9 1,844,523 - RR42_RS08805 0.23 -1.2 1,845,011 + -0.6 1,845,012 - +1.3 1,845,012 - -1.3 1,845,012 - -0.1 1,845,012 - -0.1 1,845,012 - +1.1 1,845,017 + +0.2 1,845,307 - RR42_RS08810 0.35 +0.8 1,845,441 + RR42_RS08810 0.54 +0.1 1,845,442 - RR42_RS08810 0.54 +0.5 1,845,487 - RR42_RS08810 0.60 +0.2 1,845,594 + RR42_RS08810 0.75 -0.2 1,845,594 + RR42_RS08810 0.75 -0.1 1,845,594 + RR42_RS08810 0.75 +0.3 1,845,595 - RR42_RS08810 0.76 +0.4
Or see this region's nucleotide sequence