Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS08325 and RR42_RS08330 overlap by 4 nucleotides RR42_RS08330 and RR42_RS08335 are separated by 87 nucleotides RR42_RS08335 and RR42_RS08340 are separated by 10 nucleotides
RR42_RS08325: RR42_RS08325 - hypothetical protein, at 1,762,563 to 1,763,687
_RS08325
RR42_RS08330: RR42_RS08330 - hypothetical protein, at 1,763,684 to 1,764,112
_RS08330
RR42_RS08335: RR42_RS08335 - hypothetical protein, at 1,764,200 to 1,765,069
_RS08335
RR42_RS08340: RR42_RS08340 - phage tail protein, at 1,765,080 to 1,765,481
_RS08340
Position (kb)
1763
1764
1765 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1763.035 kb on - strand, within RR42_RS08325 at 1763.037 kb on + strand, within RR42_RS08325 at 1763.145 kb on + strand, within RR42_RS08325 at 1763.146 kb on - strand, within RR42_RS08325 at 1763.196 kb on + strand, within RR42_RS08325 at 1763.196 kb on + strand, within RR42_RS08325 at 1763.317 kb on - strand, within RR42_RS08325 at 1763.464 kb on - strand, within RR42_RS08325 at 1763.464 kb on - strand, within RR42_RS08325 at 1763.583 kb on + strand at 1763.584 kb on - strand at 1763.584 kb on - strand at 1763.584 kb on - strand at 1763.584 kb on - strand at 1763.683 kb on + strand at 1763.684 kb on - strand at 1763.783 kb on + strand, within RR42_RS08330 at 1764.139 kb on + strand at 1764.208 kb on - strand at 1764.527 kb on + strand, within RR42_RS08335 at 1764.527 kb on + strand, within RR42_RS08335 at 1764.620 kb on - strand, within RR42_RS08335 at 1764.659 kb on + strand, within RR42_RS08335 at 1764.660 kb on - strand, within RR42_RS08335 at 1764.660 kb on - strand, within RR42_RS08335 at 1764.707 kb on - strand, within RR42_RS08335 at 1764.920 kb on + strand, within RR42_RS08335 at 1764.921 kb on - strand, within RR42_RS08335 at 1764.929 kb on + strand, within RR42_RS08335 at 1764.930 kb on - strand, within RR42_RS08335 at 1764.965 kb on + strand, within RR42_RS08335 at 1764.965 kb on + strand, within RR42_RS08335 at 1764.966 kb on - strand, within RR42_RS08335 at 1765.092 kb on + strand at 1765.099 kb on - strand at 1765.101 kb on + strand at 1765.101 kb on + strand at 1765.101 kb on + strand at 1765.102 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,763,035 - RR42_RS08325 0.42 +0.8 1,763,037 + RR42_RS08325 0.42 +0.2 1,763,145 + RR42_RS08325 0.52 -1.4 1,763,146 - RR42_RS08325 0.52 -0.4 1,763,196 + RR42_RS08325 0.56 +0.6 1,763,196 + RR42_RS08325 0.56 +0.9 1,763,317 - RR42_RS08325 0.67 -0.3 1,763,464 - RR42_RS08325 0.80 +0.3 1,763,464 - RR42_RS08325 0.80 -1.2 1,763,583 + +0.3 1,763,584 - -0.1 1,763,584 - -0.4 1,763,584 - +0.7 1,763,584 - -0.1 1,763,683 + -0.4 1,763,684 - -0.7 1,763,783 + RR42_RS08330 0.23 -2.6 1,764,139 + +1.2 1,764,208 - -1.9 1,764,527 + RR42_RS08335 0.38 -0.5 1,764,527 + RR42_RS08335 0.38 -0.1 1,764,620 - RR42_RS08335 0.48 -1.4 1,764,659 + RR42_RS08335 0.53 +0.7 1,764,660 - RR42_RS08335 0.53 +0.7 1,764,660 - RR42_RS08335 0.53 -2.4 1,764,707 - RR42_RS08335 0.58 +1.1 1,764,920 + RR42_RS08335 0.83 -0.0 1,764,921 - RR42_RS08335 0.83 +0.5 1,764,929 + RR42_RS08335 0.84 -3.1 1,764,930 - RR42_RS08335 0.84 +0.4 1,764,965 + RR42_RS08335 0.88 +1.8 1,764,965 + RR42_RS08335 0.88 -0.4 1,764,966 - RR42_RS08335 0.88 +0.4 1,765,092 + +0.5 1,765,099 - -0.6 1,765,101 + -0.8 1,765,101 + +0.2 1,765,101 + +1.2 1,765,102 - +0.4
Or see this region's nucleotide sequence