Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS08085 and RR42_RS08090 are separated by 35 nucleotides RR42_RS08090 and RR42_RS08095 are separated by 288 nucleotides RR42_RS08095 and RR42_RS08100 overlap by 4 nucleotides RR42_RS08100 and RR42_RS08105 are separated by 9 nucleotides
RR42_RS08085: RR42_RS08085 - hypothetical protein, at 1,729,659 to 1,730,834
_RS08085
RR42_RS08090: RR42_RS08090 - nuclease, at 1,730,870 to 1,731,664
_RS08090
RR42_RS08095: RR42_RS08095 - hypothetical protein, at 1,731,953 to 1,732,183
_RS08095
RR42_RS08100: RR42_RS08100 - hypothetical protein, at 1,732,180 to 1,732,536
_RS08100
RR42_RS08105: RR42_RS08105 - hypothetical protein, at 1,732,546 to 1,732,758
_RS08105
Position (kb)
1730
1731
1732 Strain fitness (log2 ratio)
-1
0
1 at 1730.098 kb on + strand, within RR42_RS08085 at 1730.098 kb on + strand, within RR42_RS08085 at 1730.099 kb on - strand, within RR42_RS08085 at 1730.536 kb on + strand, within RR42_RS08085 at 1730.880 kb on + strand at 1730.880 kb on + strand at 1730.907 kb on + strand at 1731.761 kb on + strand at 1731.930 kb on + strand at 1732.077 kb on + strand, within RR42_RS08095 at 1732.194 kb on + strand at 1732.517 kb on + strand at 1732.517 kb on + strand at 1732.518 kb on - strand at 1732.636 kb on + strand, within RR42_RS08105 at 1732.636 kb on + strand, within RR42_RS08105
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,730,098 + RR42_RS08085 0.37 -0.7 1,730,098 + RR42_RS08085 0.37 -1.4 1,730,099 - RR42_RS08085 0.37 -1.2 1,730,536 + RR42_RS08085 0.75 -0.3 1,730,880 + -1.5 1,730,880 + +0.3 1,730,907 + +0.9 1,731,761 + -0.1 1,731,930 + +0.8 1,732,077 + RR42_RS08095 0.54 +1.5 1,732,194 + +1.5 1,732,517 + -1.1 1,732,517 + -0.1 1,732,518 - -0.3 1,732,636 + RR42_RS08105 0.42 +0.2 1,732,636 + RR42_RS08105 0.42 +0.5
Or see this region's nucleotide sequence