Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS07510 and RR42_RS07515 are separated by 100 nucleotides RR42_RS07515 and RR42_RS07520 are separated by 232 nucleotides
RR42_RS07510: RR42_RS07510 - benzoyl-CoA-dihydrodiol lyase, at 1,603,882 to 1,605,582
_RS07510
RR42_RS07515: RR42_RS07515 - transcriptional regulator, at 1,605,683 to 1,606,621
_RS07515
RR42_RS07520: RR42_RS07520 - 4-hydroxybenzoate--CoA ligase, at 1,606,854 to 1,608,554
_RS07520
Position (kb)
1605
1606
1607 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1604.740 kb on - strand, within RR42_RS07510 at 1605.389 kb on + strand, within RR42_RS07510 at 1605.410 kb on + strand, within RR42_RS07510 at 1605.410 kb on + strand, within RR42_RS07510 at 1605.411 kb on - strand, within RR42_RS07510 at 1605.595 kb on + strand at 1605.778 kb on - strand, within RR42_RS07515 at 1605.912 kb on + strand, within RR42_RS07515 at 1605.912 kb on + strand, within RR42_RS07515 at 1605.912 kb on + strand, within RR42_RS07515 at 1605.913 kb on - strand, within RR42_RS07515 at 1605.913 kb on - strand, within RR42_RS07515 at 1606.050 kb on + strand, within RR42_RS07515 at 1606.061 kb on + strand, within RR42_RS07515 at 1606.062 kb on - strand, within RR42_RS07515 at 1606.129 kb on + strand, within RR42_RS07515 at 1606.130 kb on - strand, within RR42_RS07515 at 1606.130 kb on - strand, within RR42_RS07515 at 1606.189 kb on + strand, within RR42_RS07515 at 1606.189 kb on + strand, within RR42_RS07515 at 1606.679 kb on + strand at 1606.680 kb on - strand at 1606.680 kb on - strand at 1606.680 kb on - strand at 1606.680 kb on - strand at 1606.681 kb on + strand at 1606.682 kb on - strand at 1606.728 kb on - strand at 1606.728 kb on - strand at 1606.908 kb on - strand at 1606.908 kb on - strand at 1606.908 kb on - strand at 1606.913 kb on + strand at 1606.913 kb on + strand at 1606.914 kb on - strand at 1606.914 kb on - strand at 1606.914 kb on - strand at 1606.914 kb on - strand at 1606.916 kb on + strand at 1606.916 kb on + strand at 1606.917 kb on - strand at 1606.954 kb on - strand at 1607.016 kb on + strand at 1607.017 kb on - strand at 1607.232 kb on + strand, within RR42_RS07520 at 1607.232 kb on + strand, within RR42_RS07520 at 1607.233 kb on - strand, within RR42_RS07520 at 1607.233 kb on - strand, within RR42_RS07520 at 1607.233 kb on - strand, within RR42_RS07520 at 1607.233 kb on - strand, within RR42_RS07520 at 1607.233 kb on - strand, within RR42_RS07520 at 1607.233 kb on - strand, within RR42_RS07520 at 1607.303 kb on + strand, within RR42_RS07520 at 1607.304 kb on - strand, within RR42_RS07520 at 1607.457 kb on + strand, within RR42_RS07520 at 1607.457 kb on + strand, within RR42_RS07520 at 1607.533 kb on - strand, within RR42_RS07520 at 1607.533 kb on - strand, within RR42_RS07520
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,604,740 - RR42_RS07510 0.50 +0.0 1,605,389 + RR42_RS07510 0.89 -0.2 1,605,410 + RR42_RS07510 0.90 -0.2 1,605,410 + RR42_RS07510 0.90 +0.1 1,605,411 - RR42_RS07510 0.90 -0.4 1,605,595 + +0.4 1,605,778 - RR42_RS07515 0.10 -0.1 1,605,912 + RR42_RS07515 0.24 +0.4 1,605,912 + RR42_RS07515 0.24 -2.0 1,605,912 + RR42_RS07515 0.24 -0.3 1,605,913 - RR42_RS07515 0.24 -0.1 1,605,913 - RR42_RS07515 0.24 -0.2 1,606,050 + RR42_RS07515 0.39 -0.6 1,606,061 + RR42_RS07515 0.40 -0.5 1,606,062 - RR42_RS07515 0.40 +0.5 1,606,129 + RR42_RS07515 0.47 -0.5 1,606,130 - RR42_RS07515 0.48 -0.5 1,606,130 - RR42_RS07515 0.48 -0.1 1,606,189 + RR42_RS07515 0.54 +1.3 1,606,189 + RR42_RS07515 0.54 +0.4 1,606,679 + +0.5 1,606,680 - -1.1 1,606,680 - -0.1 1,606,680 - -1.2 1,606,680 - -1.1 1,606,681 + -0.5 1,606,682 - -1.4 1,606,728 - -0.2 1,606,728 - -2.3 1,606,908 - +0.1 1,606,908 - +0.2 1,606,908 - -0.0 1,606,913 + +1.5 1,606,913 + +2.5 1,606,914 - -1.7 1,606,914 - +0.3 1,606,914 - +0.2 1,606,914 - -1.2 1,606,916 + -1.2 1,606,916 + +0.2 1,606,917 - +0.7 1,606,954 - +0.4 1,607,016 + -0.8 1,607,017 - -0.6 1,607,232 + RR42_RS07520 0.22 +0.3 1,607,232 + RR42_RS07520 0.22 +0.2 1,607,233 - RR42_RS07520 0.22 +0.2 1,607,233 - RR42_RS07520 0.22 +0.9 1,607,233 - RR42_RS07520 0.22 +0.4 1,607,233 - RR42_RS07520 0.22 +0.1 1,607,233 - RR42_RS07520 0.22 -1.0 1,607,233 - RR42_RS07520 0.22 -0.4 1,607,303 + RR42_RS07520 0.26 +0.3 1,607,304 - RR42_RS07520 0.26 +1.1 1,607,457 + RR42_RS07520 0.35 -1.2 1,607,457 + RR42_RS07520 0.35 +0.8 1,607,533 - RR42_RS07520 0.40 +0.4 1,607,533 - RR42_RS07520 0.40 -0.2
Or see this region's nucleotide sequence