Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06565 and RR42_RS06570 are separated by 14 nucleotides RR42_RS06570 and RR42_RS06575 are separated by 202 nucleotides RR42_RS06575 and RR42_RS06580 are separated by 62 nucleotides RR42_RS06580 and RR42_RS06585 are separated by 15 nucleotides
RR42_RS06565: RR42_RS06565 - metal-binding protein, at 1,435,290 to 1,435,784
_RS06565
RR42_RS06570: RR42_RS06570 - 3-hydroxybutyrate dehydrogenase, at 1,435,799 to 1,436,575
_RS06570
RR42_RS06575: RR42_RS06575 - alpha/beta hydrolase, at 1,436,778 to 1,437,665
_RS06575
RR42_RS06580: RR42_RS06580 - aminoacrylate peracid reductase, at 1,437,728 to 1,438,090
_RS06580
RR42_RS06585: RR42_RS06585 - GTP pyrophosphokinase, at 1,438,106 to 1,440,328
_RS06585
Position (kb)
1436
1437
1438 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1435.967 kb on - strand, within RR42_RS06570 at 1436.119 kb on - strand, within RR42_RS06570 at 1436.119 kb on - strand, within RR42_RS06570 at 1436.119 kb on - strand, within RR42_RS06570 at 1436.119 kb on - strand, within RR42_RS06570 at 1436.570 kb on - strand at 1436.686 kb on + strand at 1436.687 kb on - strand at 1436.687 kb on - strand at 1436.701 kb on - strand at 1436.760 kb on + strand at 1436.760 kb on + strand at 1436.848 kb on - strand at 1436.848 kb on - strand at 1436.956 kb on - strand, within RR42_RS06575 at 1436.956 kb on - strand, within RR42_RS06575 at 1436.978 kb on - strand, within RR42_RS06575 at 1436.978 kb on - strand, within RR42_RS06575 at 1437.022 kb on - strand, within RR42_RS06575 at 1437.036 kb on + strand at 1437.037 kb on - strand, within RR42_RS06575 at 1437.037 kb on - strand, within RR42_RS06575 at 1437.037 kb on - strand, within RR42_RS06575 at 1437.037 kb on - strand, within RR42_RS06575 at 1437.037 kb on - strand, within RR42_RS06575 at 1437.222 kb on + strand, within RR42_RS06575 at 1437.222 kb on + strand, within RR42_RS06575 at 1437.223 kb on - strand, within RR42_RS06575 at 1437.223 kb on - strand, within RR42_RS06575 at 1437.388 kb on - strand, within RR42_RS06575 at 1437.499 kb on + strand, within RR42_RS06575 at 1437.639 kb on - strand at 1437.717 kb on - strand at 1437.780 kb on - strand, within RR42_RS06580 at 1437.803 kb on + strand, within RR42_RS06580 at 1437.803 kb on + strand, within RR42_RS06580 at 1437.804 kb on - strand, within RR42_RS06580 at 1437.804 kb on - strand, within RR42_RS06580 at 1438.006 kb on + strand, within RR42_RS06580 at 1438.053 kb on - strand, within RR42_RS06580 at 1438.411 kb on + strand, within RR42_RS06585 at 1438.411 kb on + strand, within RR42_RS06585 at 1438.637 kb on + strand, within RR42_RS06585 at 1438.637 kb on + strand, within RR42_RS06585
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,435,967 - RR42_RS06570 0.22 -0.8 1,436,119 - RR42_RS06570 0.41 +0.3 1,436,119 - RR42_RS06570 0.41 -0.8 1,436,119 - RR42_RS06570 0.41 +1.3 1,436,119 - RR42_RS06570 0.41 +0.8 1,436,570 - +0.1 1,436,686 + -1.3 1,436,687 - -0.3 1,436,687 - -0.0 1,436,701 - +1.2 1,436,760 + -2.1 1,436,760 + -0.8 1,436,848 - -0.1 1,436,848 - -0.3 1,436,956 - RR42_RS06575 0.20 +0.5 1,436,956 - RR42_RS06575 0.20 -0.8 1,436,978 - RR42_RS06575 0.23 -0.2 1,436,978 - RR42_RS06575 0.23 +0.9 1,437,022 - RR42_RS06575 0.27 +0.4 1,437,036 + +0.5 1,437,037 - RR42_RS06575 0.29 -0.9 1,437,037 - RR42_RS06575 0.29 -0.1 1,437,037 - RR42_RS06575 0.29 +0.5 1,437,037 - RR42_RS06575 0.29 -0.2 1,437,037 - RR42_RS06575 0.29 -0.4 1,437,222 + RR42_RS06575 0.50 -0.6 1,437,222 + RR42_RS06575 0.50 +0.5 1,437,223 - RR42_RS06575 0.50 +0.9 1,437,223 - RR42_RS06575 0.50 -1.1 1,437,388 - RR42_RS06575 0.69 +1.7 1,437,499 + RR42_RS06575 0.81 +2.5 1,437,639 - +0.4 1,437,717 - +0.4 1,437,780 - RR42_RS06580 0.14 +2.5 1,437,803 + RR42_RS06580 0.21 -0.3 1,437,803 + RR42_RS06580 0.21 +0.1 1,437,804 - RR42_RS06580 0.21 +0.1 1,437,804 - RR42_RS06580 0.21 -1.7 1,438,006 + RR42_RS06580 0.77 +1.3 1,438,053 - RR42_RS06580 0.90 +0.6 1,438,411 + RR42_RS06585 0.14 -0.1 1,438,411 + RR42_RS06585 0.14 -1.4 1,438,637 + RR42_RS06585 0.24 +0.2 1,438,637 + RR42_RS06585 0.24 -0.4
Or see this region's nucleotide sequence