Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06465 and RR42_RS06470 are separated by 202 nucleotides RR42_RS06470 and RR42_RS06475 are separated by 163 nucleotides RR42_RS06475 and RR42_RS06480 are separated by 22 nucleotides RR42_RS06480 and RR42_RS06485 are separated by 87 nucleotides
RR42_RS06465: RR42_RS06465 - O-acetylhomoserine aminocarboxypropyltransferase, at 1,414,258 to 1,415,568
_RS06465
RR42_RS06470: RR42_RS06470 - hypothetical protein, at 1,415,771 to 1,416,217
_RS06470
RR42_RS06475: RR42_RS06475 - glycerol acyltransferase, at 1,416,381 to 1,418,279
_RS06475
RR42_RS06480: RR42_RS06480 - glutathione S-transferase, at 1,418,302 to 1,419,021
_RS06480
RR42_RS06485: RR42_RS06485 - chorismate synthase, at 1,419,109 to 1,420,227
_RS06485
Position (kb)
1416
1417
1418
1419 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1415.405 kb on + strand, within RR42_RS06465 at 1415.406 kb on - strand, within RR42_RS06465 at 1415.461 kb on - strand at 1415.582 kb on - strand at 1415.605 kb on + strand at 1415.605 kb on + strand at 1415.605 kb on + strand at 1415.605 kb on + strand at 1415.605 kb on + strand at 1415.606 kb on - strand at 1415.606 kb on - strand at 1415.606 kb on - strand at 1415.606 kb on - strand at 1415.606 kb on - strand at 1415.606 kb on - strand at 1415.636 kb on + strand at 1415.637 kb on - strand at 1415.728 kb on + strand at 1415.728 kb on + strand at 1415.729 kb on - strand at 1415.731 kb on + strand at 1415.731 kb on + strand at 1415.732 kb on - strand at 1415.732 kb on - strand at 1415.732 kb on - strand at 1415.732 kb on - strand at 1415.814 kb on - strand at 1415.816 kb on - strand, within RR42_RS06470 at 1415.855 kb on - strand, within RR42_RS06470 at 1416.123 kb on - strand, within RR42_RS06470 at 1416.182 kb on + strand at 1416.310 kb on - strand at 1416.316 kb on + strand at 1416.325 kb on + strand at 1416.378 kb on + strand at 1416.378 kb on + strand at 1416.378 kb on + strand at 1416.504 kb on + strand at 1416.504 kb on + strand at 1416.504 kb on + strand at 1416.504 kb on + strand at 1416.504 kb on + strand at 1416.509 kb on + strand at 1416.510 kb on - strand at 1416.510 kb on - strand at 1416.671 kb on + strand, within RR42_RS06475 at 1416.671 kb on + strand, within RR42_RS06475 at 1416.671 kb on + strand, within RR42_RS06475 at 1416.672 kb on - strand, within RR42_RS06475 at 1416.672 kb on - strand, within RR42_RS06475 at 1416.679 kb on + strand, within RR42_RS06475 at 1416.680 kb on - strand, within RR42_RS06475 at 1416.680 kb on - strand, within RR42_RS06475 at 1416.781 kb on - strand, within RR42_RS06475 at 1416.928 kb on - strand, within RR42_RS06475 at 1416.928 kb on - strand, within RR42_RS06475 at 1417.450 kb on - strand, within RR42_RS06475 at 1417.647 kb on + strand, within RR42_RS06475 at 1417.647 kb on + strand, within RR42_RS06475 at 1417.647 kb on + strand, within RR42_RS06475 at 1417.647 kb on + strand, within RR42_RS06475 at 1417.647 kb on + strand, within RR42_RS06475 at 1417.647 kb on + strand, within RR42_RS06475 at 1417.647 kb on + strand, within RR42_RS06475 at 1417.648 kb on - strand, within RR42_RS06475 at 1417.648 kb on - strand, within RR42_RS06475 at 1417.648 kb on - strand, within RR42_RS06475 at 1417.648 kb on - strand, within RR42_RS06475 at 1417.648 kb on - strand, within RR42_RS06475 at 1417.653 kb on + strand, within RR42_RS06475 at 1417.653 kb on + strand, within RR42_RS06475 at 1417.654 kb on - strand, within RR42_RS06475 at 1417.706 kb on + strand, within RR42_RS06475 at 1417.707 kb on - strand, within RR42_RS06475 at 1417.713 kb on + strand, within RR42_RS06475 at 1417.951 kb on - strand, within RR42_RS06475 at 1417.951 kb on - strand, within RR42_RS06475 at 1418.314 kb on + strand at 1418.314 kb on + strand at 1418.314 kb on + strand at 1418.452 kb on + strand, within RR42_RS06480 at 1418.519 kb on - strand, within RR42_RS06480 at 1418.596 kb on - strand, within RR42_RS06480 at 1418.596 kb on - strand, within RR42_RS06480 at 1418.602 kb on + strand, within RR42_RS06480 at 1418.602 kb on + strand, within RR42_RS06480 at 1418.962 kb on + strand at 1418.962 kb on + strand at 1418.962 kb on + strand at 1418.962 kb on + strand at 1418.962 kb on + strand at 1418.963 kb on - strand at 1418.963 kb on - strand at 1418.989 kb on + strand at 1419.060 kb on + strand at 1419.061 kb on - strand at 1419.061 kb on - strand at 1419.067 kb on + strand at 1419.138 kb on + strand at 1419.138 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,415,405 + RR42_RS06465 0.87 -1.4 1,415,406 - RR42_RS06465 0.88 -0.4 1,415,461 - -2.9 1,415,582 - +0.5 1,415,605 + -0.2 1,415,605 + -1.6 1,415,605 + -1.0 1,415,605 + +0.3 1,415,605 + +0.4 1,415,606 - -1.2 1,415,606 - +0.5 1,415,606 - +0.1 1,415,606 - +1.1 1,415,606 - +0.5 1,415,606 - -0.3 1,415,636 + +0.2 1,415,637 - +0.2 1,415,728 + -0.1 1,415,728 + -0.2 1,415,729 - -1.0 1,415,731 + -0.3 1,415,731 + -0.6 1,415,732 - +1.4 1,415,732 - -0.7 1,415,732 - -0.9 1,415,732 - -1.6 1,415,814 - -1.8 1,415,816 - RR42_RS06470 0.10 -0.7 1,415,855 - RR42_RS06470 0.19 +0.3 1,416,123 - RR42_RS06470 0.79 -1.0 1,416,182 + -0.4 1,416,310 - -0.2 1,416,316 + -0.9 1,416,325 + -0.8 1,416,378 + -1.4 1,416,378 + -1.5 1,416,378 + +0.1 1,416,504 + -0.3 1,416,504 + -0.5 1,416,504 + +0.3 1,416,504 + +0.5 1,416,504 + -1.3 1,416,509 + -0.7 1,416,510 - +0.4 1,416,510 - -0.7 1,416,671 + RR42_RS06475 0.15 -1.1 1,416,671 + RR42_RS06475 0.15 +0.4 1,416,671 + RR42_RS06475 0.15 -1.5 1,416,672 - RR42_RS06475 0.15 -0.3 1,416,672 - RR42_RS06475 0.15 -0.8 1,416,679 + RR42_RS06475 0.16 -2.3 1,416,680 - RR42_RS06475 0.16 -2.0 1,416,680 - RR42_RS06475 0.16 +0.2 1,416,781 - RR42_RS06475 0.21 -1.1 1,416,928 - RR42_RS06475 0.29 -1.3 1,416,928 - RR42_RS06475 0.29 -2.1 1,417,450 - RR42_RS06475 0.56 +0.2 1,417,647 + RR42_RS06475 0.67 -0.2 1,417,647 + RR42_RS06475 0.67 -0.6 1,417,647 + RR42_RS06475 0.67 -1.3 1,417,647 + RR42_RS06475 0.67 -0.3 1,417,647 + RR42_RS06475 0.67 -0.1 1,417,647 + RR42_RS06475 0.67 -2.1 1,417,647 + RR42_RS06475 0.67 -1.8 1,417,648 - RR42_RS06475 0.67 -1.2 1,417,648 - RR42_RS06475 0.67 -0.1 1,417,648 - RR42_RS06475 0.67 -1.5 1,417,648 - RR42_RS06475 0.67 -0.5 1,417,648 - RR42_RS06475 0.67 -1.3 1,417,653 + RR42_RS06475 0.67 -1.7 1,417,653 + RR42_RS06475 0.67 -0.1 1,417,654 - RR42_RS06475 0.67 -0.8 1,417,706 + RR42_RS06475 0.70 -1.2 1,417,707 - RR42_RS06475 0.70 -1.1 1,417,713 + RR42_RS06475 0.70 -0.6 1,417,951 - RR42_RS06475 0.83 -0.1 1,417,951 - RR42_RS06475 0.83 -0.3 1,418,314 + -0.3 1,418,314 + +0.8 1,418,314 + -0.8 1,418,452 + RR42_RS06480 0.21 -1.1 1,418,519 - RR42_RS06480 0.30 +0.7 1,418,596 - RR42_RS06480 0.41 +2.5 1,418,596 - RR42_RS06480 0.41 -0.6 1,418,602 + RR42_RS06480 0.42 -0.1 1,418,602 + RR42_RS06480 0.42 -1.4 1,418,962 + +0.6 1,418,962 + -1.3 1,418,962 + -1.8 1,418,962 + -0.9 1,418,962 + -1.4 1,418,963 - -1.2 1,418,963 - +0.2 1,418,989 + +0.0 1,419,060 + -0.1 1,419,061 - -0.1 1,419,061 - -1.4 1,419,067 + -1.9 1,419,138 + +0.4 1,419,138 + +0.6
Or see this region's nucleotide sequence