Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06270 and RR42_RS06275 are separated by 92 nucleotides RR42_RS06275 and RR42_RS06280 are separated by 95 nucleotides RR42_RS06280 and RR42_RS06285 overlap by 4 nucleotides
RR42_RS06270: RR42_RS06270 - cupin, at 1,374,634 to 1,375,761
_RS06270
RR42_RS06275: RR42_RS06275 - peptidylprolyl isomerase, at 1,375,854 to 1,376,387
_RS06275
RR42_RS06280: RR42_RS06280 - membrane protein, at 1,376,483 to 1,377,541
_RS06280
RR42_RS06285: RR42_RS06285 - DNA mismatch repair protein MutS, at 1,377,538 to 1,380,180
_RS06285
Position (kb)
1376
1377
1378 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1375.734 kb on + strand at 1375.824 kb on - strand at 1375.824 kb on - strand at 1375.824 kb on - strand at 1375.825 kb on + strand at 1375.826 kb on - strand at 1375.890 kb on + strand at 1375.890 kb on + strand at 1375.890 kb on + strand at 1375.890 kb on + strand at 1375.891 kb on - strand at 1375.891 kb on - strand at 1375.948 kb on - strand, within RR42_RS06275 at 1375.948 kb on - strand, within RR42_RS06275 at 1375.948 kb on - strand, within RR42_RS06275 at 1376.061 kb on + strand, within RR42_RS06275 at 1376.062 kb on - strand, within RR42_RS06275 at 1376.083 kb on + strand, within RR42_RS06275 at 1376.083 kb on + strand, within RR42_RS06275 at 1376.175 kb on + strand, within RR42_RS06275 at 1376.176 kb on - strand, within RR42_RS06275 at 1376.176 kb on - strand, within RR42_RS06275 at 1376.176 kb on - strand, within RR42_RS06275 at 1376.176 kb on - strand, within RR42_RS06275 at 1376.428 kb on + strand at 1376.485 kb on - strand at 1376.485 kb on - strand at 1376.559 kb on + strand at 1376.946 kb on + strand, within RR42_RS06280 at 1376.947 kb on - strand, within RR42_RS06280 at 1377.022 kb on - strand, within RR42_RS06280 at 1377.034 kb on + strand, within RR42_RS06280 at 1377.034 kb on + strand, within RR42_RS06280 at 1377.035 kb on - strand, within RR42_RS06280 at 1377.035 kb on - strand, within RR42_RS06280 at 1377.193 kb on - strand, within RR42_RS06280 at 1377.193 kb on - strand, within RR42_RS06280 at 1377.221 kb on + strand, within RR42_RS06280 at 1377.551 kb on - strand at 1377.643 kb on + strand at 1377.643 kb on + strand at 1377.643 kb on + strand at 1377.643 kb on + strand at 1377.643 kb on + strand at 1377.644 kb on - strand at 1377.644 kb on - strand at 1377.644 kb on - strand at 1377.644 kb on - strand at 1377.887 kb on + strand, within RR42_RS06285 at 1377.887 kb on + strand at 1377.888 kb on - strand, within RR42_RS06285 at 1378.013 kb on + strand, within RR42_RS06285 at 1378.013 kb on + strand, within RR42_RS06285 at 1378.014 kb on - strand, within RR42_RS06285 at 1378.032 kb on + strand, within RR42_RS06285 at 1378.032 kb on + strand, within RR42_RS06285 at 1378.033 kb on - strand, within RR42_RS06285 at 1378.279 kb on + strand, within RR42_RS06285 at 1378.280 kb on - strand, within RR42_RS06285 at 1378.283 kb on + strand, within RR42_RS06285 at 1378.283 kb on + strand, within RR42_RS06285 at 1378.283 kb on + strand, within RR42_RS06285 at 1378.283 kb on + strand, within RR42_RS06285 at 1378.283 kb on + strand, within RR42_RS06285 at 1378.283 kb on + strand, within RR42_RS06285 at 1378.284 kb on - strand, within RR42_RS06285 at 1378.284 kb on - strand, within RR42_RS06285 at 1378.341 kb on + strand, within RR42_RS06285
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,375,734 + +1.3 1,375,824 - +0.7 1,375,824 - +0.1 1,375,824 - -1.2 1,375,825 + -0.6 1,375,826 - -0.6 1,375,890 + -0.8 1,375,890 + -2.3 1,375,890 + +0.5 1,375,890 + +2.0 1,375,891 - -1.6 1,375,891 - +0.6 1,375,948 - RR42_RS06275 0.18 +0.1 1,375,948 - RR42_RS06275 0.18 -0.8 1,375,948 - RR42_RS06275 0.18 -1.2 1,376,061 + RR42_RS06275 0.39 +0.2 1,376,062 - RR42_RS06275 0.39 -2.6 1,376,083 + RR42_RS06275 0.43 -1.0 1,376,083 + RR42_RS06275 0.43 -0.6 1,376,175 + RR42_RS06275 0.60 -0.5 1,376,176 - RR42_RS06275 0.60 -0.5 1,376,176 - RR42_RS06275 0.60 +0.1 1,376,176 - RR42_RS06275 0.60 -2.3 1,376,176 - RR42_RS06275 0.60 +0.1 1,376,428 + -2.1 1,376,485 - -1.6 1,376,485 - +0.2 1,376,559 + -0.3 1,376,946 + RR42_RS06280 0.44 +0.4 1,376,947 - RR42_RS06280 0.44 +1.3 1,377,022 - RR42_RS06280 0.51 +0.5 1,377,034 + RR42_RS06280 0.52 -0.1 1,377,034 + RR42_RS06280 0.52 +0.5 1,377,035 - RR42_RS06280 0.52 -0.5 1,377,035 - RR42_RS06280 0.52 -0.7 1,377,193 - RR42_RS06280 0.67 +0.1 1,377,193 - RR42_RS06280 0.67 +0.7 1,377,221 + RR42_RS06280 0.70 -1.2 1,377,551 - +0.2 1,377,643 + +0.5 1,377,643 + +0.8 1,377,643 + -1.8 1,377,643 + -0.1 1,377,643 + +0.5 1,377,644 - -0.1 1,377,644 - -0.5 1,377,644 - -2.5 1,377,644 - -0.2 1,377,887 + RR42_RS06285 0.13 -1.2 1,377,887 + +1.5 1,377,888 - RR42_RS06285 0.13 +0.5 1,378,013 + RR42_RS06285 0.18 -1.5 1,378,013 + RR42_RS06285 0.18 +0.4 1,378,014 - RR42_RS06285 0.18 -0.5 1,378,032 + RR42_RS06285 0.19 -1.3 1,378,032 + RR42_RS06285 0.19 -0.3 1,378,033 - RR42_RS06285 0.19 -0.3 1,378,279 + RR42_RS06285 0.28 -1.1 1,378,280 - RR42_RS06285 0.28 -1.0 1,378,283 + RR42_RS06285 0.28 -0.2 1,378,283 + RR42_RS06285 0.28 -1.7 1,378,283 + RR42_RS06285 0.28 +0.2 1,378,283 + RR42_RS06285 0.28 -0.8 1,378,283 + RR42_RS06285 0.28 +1.3 1,378,283 + RR42_RS06285 0.28 -0.5 1,378,284 - RR42_RS06285 0.28 +0.0 1,378,284 - RR42_RS06285 0.28 +0.9 1,378,341 + RR42_RS06285 0.30 -0.2
Or see this region's nucleotide sequence