Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05960 and RR42_RS05965 are separated by 84 nucleotides RR42_RS05965 and RR42_RS05970 are separated by 72 nucleotides RR42_RS05970 and RR42_RS05975 overlap by 1 nucleotides
RR42_RS05960: RR42_RS05960 - multidrug DMT transporter permease, at 1,311,292 to 1,312,194
_RS05960
RR42_RS05965: RR42_RS05965 - malonic semialdehyde reductase, at 1,312,279 to 1,312,866
_RS05965
RR42_RS05970: RR42_RS05970 - endonuclease III, at 1,312,939 to 1,313,583
_RS05970
RR42_RS05975: RR42_RS05975 - ferredoxin, at 1,313,583 to 1,314,404
_RS05975
Position (kb)
1312
1313
1314 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1311.962 kb on + strand, within RR42_RS05960 at 1312.236 kb on + strand at 1312.237 kb on - strand at 1312.237 kb on - strand at 1312.237 kb on - strand at 1312.240 kb on + strand at 1312.240 kb on + strand at 1312.240 kb on + strand at 1312.241 kb on - strand at 1312.241 kb on - strand at 1312.241 kb on - strand at 1312.329 kb on + strand at 1312.329 kb on + strand at 1312.330 kb on - strand at 1312.667 kb on - strand, within RR42_RS05965 at 1312.889 kb on - strand at 1312.889 kb on - strand at 1312.889 kb on - strand at 1312.970 kb on + strand at 1313.030 kb on + strand, within RR42_RS05970 at 1313.030 kb on + strand, within RR42_RS05970 at 1313.031 kb on - strand, within RR42_RS05970 at 1313.163 kb on + strand, within RR42_RS05970 at 1313.163 kb on + strand at 1313.163 kb on + strand, within RR42_RS05970 at 1313.164 kb on - strand, within RR42_RS05970 at 1313.164 kb on - strand, within RR42_RS05970 at 1313.164 kb on - strand, within RR42_RS05970 at 1313.209 kb on + strand, within RR42_RS05970 at 1313.314 kb on + strand, within RR42_RS05970 at 1313.314 kb on + strand, within RR42_RS05970 at 1313.314 kb on + strand, within RR42_RS05970 at 1313.314 kb on + strand, within RR42_RS05970 at 1313.314 kb on + strand, within RR42_RS05970 at 1313.315 kb on - strand, within RR42_RS05970 at 1313.315 kb on - strand, within RR42_RS05970 at 1313.315 kb on - strand, within RR42_RS05970 at 1313.340 kb on - strand, within RR42_RS05970 at 1313.340 kb on - strand, within RR42_RS05970 at 1313.360 kb on + strand, within RR42_RS05970 at 1313.360 kb on + strand, within RR42_RS05970 at 1313.361 kb on - strand, within RR42_RS05970 at 1313.361 kb on - strand, within RR42_RS05970 at 1313.361 kb on - strand, within RR42_RS05970 at 1313.361 kb on - strand, within RR42_RS05970 at 1313.361 kb on - strand, within RR42_RS05970 at 1313.361 kb on - strand, within RR42_RS05970 at 1313.361 kb on - strand, within RR42_RS05970 at 1313.476 kb on + strand, within RR42_RS05970 at 1313.476 kb on + strand, within RR42_RS05970 at 1313.477 kb on - strand, within RR42_RS05970 at 1313.477 kb on - strand, within RR42_RS05970 at 1313.477 kb on - strand, within RR42_RS05970 at 1313.477 kb on - strand, within RR42_RS05970 at 1313.507 kb on + strand, within RR42_RS05970 at 1313.507 kb on + strand, within RR42_RS05970 at 1313.507 kb on + strand, within RR42_RS05970 at 1313.508 kb on - strand, within RR42_RS05970 at 1313.508 kb on - strand, within RR42_RS05970 at 1313.585 kb on - strand at 1313.585 kb on - strand at 1313.648 kb on + strand at 1313.927 kb on - strand, within RR42_RS05975 at 1314.071 kb on + strand, within RR42_RS05975 at 1314.071 kb on + strand, within RR42_RS05975 at 1314.310 kb on + strand, within RR42_RS05975
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,311,962 + RR42_RS05960 0.74 -1.3 1,312,236 + +0.1 1,312,237 - -0.2 1,312,237 - -0.2 1,312,237 - +0.7 1,312,240 + -0.2 1,312,240 + -0.2 1,312,240 + +0.5 1,312,241 - -0.3 1,312,241 - +1.0 1,312,241 - -0.4 1,312,329 + +3.1 1,312,329 + +0.3 1,312,330 - +0.9 1,312,667 - RR42_RS05965 0.66 +0.5 1,312,889 - -0.6 1,312,889 - -0.3 1,312,889 - +0.9 1,312,970 + -0.8 1,313,030 + RR42_RS05970 0.14 -0.2 1,313,030 + RR42_RS05970 0.14 +0.3 1,313,031 - RR42_RS05970 0.14 -0.0 1,313,163 + RR42_RS05970 0.35 +0.4 1,313,163 + +1.5 1,313,163 + RR42_RS05970 0.35 +0.0 1,313,164 - RR42_RS05970 0.35 -0.8 1,313,164 - RR42_RS05970 0.35 +0.2 1,313,164 - RR42_RS05970 0.35 -0.8 1,313,209 + RR42_RS05970 0.42 -0.6 1,313,314 + RR42_RS05970 0.58 +0.3 1,313,314 + RR42_RS05970 0.58 -0.8 1,313,314 + RR42_RS05970 0.58 -1.3 1,313,314 + RR42_RS05970 0.58 -1.1 1,313,314 + RR42_RS05970 0.58 +0.2 1,313,315 - RR42_RS05970 0.58 -1.3 1,313,315 - RR42_RS05970 0.58 +0.5 1,313,315 - RR42_RS05970 0.58 -0.2 1,313,340 - RR42_RS05970 0.62 -0.7 1,313,340 - RR42_RS05970 0.62 -0.2 1,313,360 + RR42_RS05970 0.65 -0.7 1,313,360 + RR42_RS05970 0.65 +1.5 1,313,361 - RR42_RS05970 0.65 +0.9 1,313,361 - RR42_RS05970 0.65 +0.4 1,313,361 - RR42_RS05970 0.65 -0.8 1,313,361 - RR42_RS05970 0.65 -0.9 1,313,361 - RR42_RS05970 0.65 -1.5 1,313,361 - RR42_RS05970 0.65 -0.8 1,313,361 - RR42_RS05970 0.65 +0.8 1,313,476 + RR42_RS05970 0.83 -1.7 1,313,476 + RR42_RS05970 0.83 -1.6 1,313,477 - RR42_RS05970 0.83 -0.2 1,313,477 - RR42_RS05970 0.83 -1.3 1,313,477 - RR42_RS05970 0.83 -0.0 1,313,477 - RR42_RS05970 0.83 -0.6 1,313,507 + RR42_RS05970 0.88 -0.5 1,313,507 + RR42_RS05970 0.88 -0.3 1,313,507 + RR42_RS05970 0.88 -0.5 1,313,508 - RR42_RS05970 0.88 +1.0 1,313,508 - RR42_RS05970 0.88 -0.5 1,313,585 - -0.2 1,313,585 - -0.6 1,313,648 + +0.2 1,313,927 - RR42_RS05975 0.42 -0.2 1,314,071 + RR42_RS05975 0.59 -0.8 1,314,071 + RR42_RS05975 0.59 -0.2 1,314,310 + RR42_RS05975 0.88 +2.5
Or see this region's nucleotide sequence