Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05110 and RR42_RS05115 are separated by 153 nucleotides RR42_RS05115 and RR42_RS05120 are separated by 138 nucleotides
RR42_RS05110: RR42_RS05110 - betaine-aldehyde dehydrogenase, at 1,127,574 to 1,129,043
_RS05110
RR42_RS05115: RR42_RS05115 - LysR family transcriptional regulator, at 1,129,197 to 1,130,165
_RS05115
RR42_RS05120: RR42_RS05120 - ABC transporter substrate-binding protein, at 1,130,304 to 1,131,284
_RS05120
Position (kb)
1129
1130
1131 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 1128.619 kb on + strand, within RR42_RS05110 at 1128.620 kb on - strand, within RR42_RS05110 at 1128.658 kb on + strand, within RR42_RS05110 at 1128.660 kb on + strand, within RR42_RS05110 at 1128.660 kb on + strand, within RR42_RS05110 at 1128.661 kb on - strand, within RR42_RS05110 at 1128.847 kb on - strand, within RR42_RS05110 at 1129.097 kb on + strand at 1129.098 kb on - strand at 1129.098 kb on - strand at 1129.098 kb on - strand at 1129.100 kb on - strand at 1129.100 kb on - strand at 1129.123 kb on + strand at 1129.629 kb on - strand, within RR42_RS05115 at 1129.888 kb on + strand, within RR42_RS05115 at 1129.889 kb on - strand, within RR42_RS05115 at 1130.242 kb on - strand at 1130.528 kb on + strand, within RR42_RS05120 at 1130.548 kb on + strand, within RR42_RS05120 at 1130.668 kb on + strand, within RR42_RS05120 at 1130.809 kb on - strand, within RR42_RS05120 at 1131.010 kb on + strand, within RR42_RS05120 at 1131.011 kb on - strand, within RR42_RS05120 at 1131.038 kb on - strand, within RR42_RS05120
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,128,619 + RR42_RS05110 0.71 -1.1 1,128,620 - RR42_RS05110 0.71 -0.2 1,128,658 + RR42_RS05110 0.74 -0.2 1,128,660 + RR42_RS05110 0.74 +0.4 1,128,660 + RR42_RS05110 0.74 +0.2 1,128,661 - RR42_RS05110 0.74 -0.7 1,128,847 - RR42_RS05110 0.87 -0.6 1,129,097 + -0.4 1,129,098 - -0.8 1,129,098 - -0.5 1,129,098 - -0.0 1,129,100 - -0.5 1,129,100 - -0.1 1,129,123 + +0.0 1,129,629 - RR42_RS05115 0.45 -0.6 1,129,888 + RR42_RS05115 0.71 +3.9 1,129,889 - RR42_RS05115 0.71 +0.6 1,130,242 - -0.1 1,130,528 + RR42_RS05120 0.23 -1.0 1,130,548 + RR42_RS05120 0.25 +0.5 1,130,668 + RR42_RS05120 0.37 -0.7 1,130,809 - RR42_RS05120 0.51 -1.0 1,131,010 + RR42_RS05120 0.72 +0.9 1,131,011 - RR42_RS05120 0.72 +1.2 1,131,038 - RR42_RS05120 0.75 +0.2
Or see this region's nucleotide sequence